Antioxidant activity of Echinophora platyloba DC essential oil: a comparative study on four different methods

Iranian Journal of Veterinary Science and Technology

مقاله 7، دوره 8، شماره 1 - شماره پیاپی 14، تیر 2016، صفحه 47-52   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v8i1.50620

نویسندگان

Ali Ehsani¹؛ Mohammad Hashemi^{* 2}؛ Asma Afshari²

¹Tabriz University of Medical Sciences

²Mashhad University of Medical Sciences

چکیده

The present study is conducted in order to investigate the antioxidant capacity of Echinophora platyloba DC (Duncan) essential oil (EO), growing wild in west Azerbaijan, Iran, with four different assays. The aerial parts of Echinophora platyloba DC were provided from Maraghe city district, northwest of Iran and its phytochemicals were determined using GC-MS analysis. In order to evaluate and compare the antioxidant activity of the EO, various concentrations (1, 2.5, 5 and 10 mg.ml-1) of the oil and reference antioxidants (ascorbic acid and BHT) were analyzed by four different assays such as 2,2’-azinobis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric reducing antioxidant power (FRAP) and β-carotene bleaching test (BCBT). The oil exhibited moderate antioxidant activity with a dose-response in DPPH and ABTS assays and it
exhibited strong antioxidant activity in FRAP and BCBT assays. It is concluded that FRAP and BCBT assays are more suitable spectrometric assays for antioxidant capacity evaluation of Echinophora platyloba.

کلیدواژه‌ها

Echinophora platyloba؛ ABTS؛ DPPH؛ FRAP؛ β-carotene bleaching test

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