Comparison of application of combination immunomagnetic separation and chromagar salmonella medium with conventional culture method for rapid isolation and detection of Salmonella in bovine diarrheic samples

Iranian Journal of Veterinary Science and Technology

مقاله 8، دوره 8، شماره 1 - شماره پیاپی 14، تیر 2016، صفحه 53-57   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v8i1.48417

نویسنده

nemat shams^*

Lorestan University

چکیده

Various techniques and culture media were developed for rapid identification of Salmonella serovars. However, there are still problems with their sensitivity and specificity. In an attempt to reduce the time spent to obtain a result and to minimize the problems associated with rapid detection systems such as interference from food ingredients debris, micro flora in feces, and lack of sensitivity, there has been a lot of interest in the development of separation and concentration techniques prior to detection of pathogenic organisms. Various techniques have been utilized for this purpose including: filtration, centrifugation, and lectin-based biosorbents. However, the most successful of the approaches for separation and concentration of target organisms has been the use of Immunomagnetic Separation (IMS). This study was conducted with the objective of comparing the conventional microbiological methods to detect salmonella in diarrheic samples with Immunomagnetic separation combined with chromagar
salmonellae medium (IMS-CAS). Of the 400 fecal samples tested by the conventional microbiological and IMS-CAS methods, 33 (8.25%) was culture positive for Salmonella serotypes. The IMS-CAS method gave better results than the conventional microbiological method with less false-positive colonies. Sensitivities for the conventional microbiological method and the IMS-CAS were 100%. The specificity of the IMS-CAS method (99.73%) was significantly higher than that of the conventional microbiological method (94.55%). The use of plating IMS on CAS medium demonstrated high levels of sensitivity and specificity and reduced the time to final identification of Salmonella spp., resulting in substantial cost savings. It can be recommended for the primary isolation of Salmonella spp. from stool specimens.

کلیدواژه‌ها

Salmonella؛ Immunomagnetic separation؛ CHROMagar Salmonellae Medium

مراجع

Amani, J., Mirhosseini, S.A. and Imani Fooladi, A. A. (2015) A Review Approaches to Iden tify Enteric Bacterial Pathogens. Jundishapur Journal of Microbiology 8, (2):e17473.

Blackburn, C.W. (1993) Rapid and alternative methods for the detection of salmonellas in foods. Journal of Applied Bacteriology 75, 199–214.

Brandão, D., Liebana, S., Campoy, S., Cortes, P., Alegret, S., Pividori, M.I.(2013) Electro-chemical magneto-immunosensing of Salmonella based on nano and micro-sized mag-netic particles.8th Ibero-American Congress on Sensors , Journal of Physics: Conference Series 421- 012020.

Chao-Yu, H., Bing-Mu, H., Tien-Yu,C., Tsui-Kang, Hsu., ShuMin, S.,Yi-Chou, Chiu., Hung-Jen,W., Wen-Tsai, J., ChengWei, F., Jyh-Larng, Chen.(2014) Evaluation of Immuno-magnetic Separation for the Detection of Salmonella in Surface Waters by Polymerase Chain Reaction. International Journal of Environmental Research and Public Health 11, 9811-9821

Coleman, D.J., Chick, K.E. and Nye, K.J. (1995a) An evaluation

of immunomagnetic separa-tion for the detection of salmonellas in raw chicken carcasses. Letters in Applied Mi-crobiology 21, 152–154.

Coleman, D.J., Nye, K.J., Chick, K.E. and Gagg, C.M. (1995b) A comparison of immuno-magnetic separation plus enrichment with conventional Salmonella culture in the exam-ination of raw sausages. Letters in Applied Microbiology 21, 249–251.

Conceicao, R.S., Moreira, A.N., Ramos, R.J., Goularte, F.L., Carvalhal, J.B., Aleixo, J.A.G. (2008)Detection of salmonella spp in chicken cuts using immunomagnetic sepa-ration. Brazilian Journal of Microbiology 39, 173–177.

Dziadkowiec, D., Mansfield, L.P. and Forsythe, S.J. (1995) The detection of Salmonella in skimmed milk powder enrichments using conventional methods and immunomagnetic separation. Letters in Applied Microbiology 20, 361–364.

Fengying, D., Miao, Z., Bingbing, H., Yongjun, S., Qunwei, Tang., Meihong, D., Xin, Z.(2015) Immunomagnetic nanoparticles based on a hydrophilic polymer coating for sensitive detection of Salmonella in raw milk by polymerase chain reaction. RSC Ad-vances 5, 3574-3580.

Fung, D.Y. (2002) Predictions for rapid methods and automation in food microbiology. Journal of AOAC International 85, 1000–1002.

Koluman, A., CeliK, G., Unlu, T. (2012) Salmonella identification from foods in eight hours: A prototype study with Salmonella Typhimurium. Iranian Journal of Microbiology 4 (1), 15-24.

Maddocks, S., Olma, T and Chen, S. (2002) Comparison of CHROMagar Salmonella Medi-um and Xylose-Lysine-Desoxycholate and Salmonella-Shigella Agars for Isolation of Salmonella Strains from Stool Samples. Journal of Clinical Microbiology, 40(8), 2999–3003.

Odumeru, J. A., Leon-Velarde C. G. (2012)Salmonella Detection Methods for Food and Food Ingredients, Salmonella - A Dangerous Foodborne Pathogen, InTech, pp. 373-392.

Olsvik, O., Popovic, T., Skjerve, E., Cudjoe, K.S., Hornes, E.,Ugelstad, J. and Uhlen, M. (1994) Magnetic separation techniques in diagnostic microbiology. Clinical Microbiol-ogy Review 7, 43–54.

Rita de Cassia dos Santos da Conceição, Ângela Nunes Moreira, Roberta Juliano Ramos, Fa-biana Lemos Goularte, Jose Beiro Carvalhal, and Jose Antonio Guimarães Aleixo.(2008) Detection of Salmonella spp in chicken cuts using immunomagnetic separation. Brazilian Journal of Microbiology 39(1), 173–177.

Safarik, I., Safarikova, M. and Forsythe, S.J. (1995) The application of magnetic separations in applied microbiology. Journal of Applied Bacteriology 78, 575–585.

Shaw, S.J., Blais, B.W., Nundy, D. (1998 Performance of the Dynabeads anti-Salmonella sys-tem in the detection of Salmonella species in foods, animal feeds, and environmental samples. Journal of Food Protection 61, 1507–1510.

Salehi T.Z., Tadjbakhsh, H., Atashparvar, N., Nadalian, M.G., Mahzounieh M.R.(2007) De-tection and identification of Salmonella Typhimurium in bovine diarrhoeic fecal sam-ples by immunomagnetic separation and multiplex PCR assay. Zoonoses Public Health. 54(6-7), 231-236.

Taha, E.G., Mohamed, A., Srivastava, K. K., Reddy, P. G.(2010) Rapid Detection of Salmo-nella in Chicken Meat Using Immunomagnetic Separation, CHROMagar, ELISA and Real-time Polymerase Chain Reaction (RTPCR). International Journal of Poultry Sci-ence 9 (9), 831-835.

Yang, L.J., Li, Y.B. (2006) Detection of viable Salmonella using microelectrode-based capaci-tance measurement coupled with immunomagnetic separation. Journal of Microbiolog-ical Methods, 64, 9-16.

Zheng, Q., Mikš-Krajnik, M., Yang, Y., Xu, W., Yuk, H.G.(2014) Real-time PCR method combined with immunomagnetic separation for detecting healthy and heat-injured Salmonella Typhimurium on raw duck wings. International Journal of Food Microbiol-ogy 1(186), 6-13.