Putative RFLP Analysis Between HSVd-sycv and Closely Related Variants

Journal of Cell and Molecular Research

Article 1, Volume 8, Issue 1 - Serial Number 15, January 0, Pages 35-36   

Document Type: Letters / Commentaries

DOI: 10.22067/jcmr.v8i1.50824

Abstract

Yellow corky vein is a prevalent disease among navel oranges in the Fars province of Iran. Previously we showed
that a variant of Hop stunt viroid (HSVd-sycv) was associated with the disease. It was closely related to citrus variant
of HSVd from Japan (HSVd-cit8) and with 93.7% homology with lime yellow corky vein variant of HSVd (HSVd–
lycv). In this study, putative RFLP (Restriction Fragment Length Polymorphism) analysis using Vector NTI program
(version 9.0.0) showed that some restriction enzymes could cut HSVd-sycv but not HSVd-lycv. On the other hand,
M.Ngo BIX and FauI which could cut HSVd-lycv at positions of nt 53 and 97, respectively, could not cut HSVdsycv.
Likewise, some restriction enzymes could cut HSVd-cit8 but could not cut HSVd-sycv. Therefore these variants
can be recognized by these restriction enzymes.

Keywords

HSVd-sycv; HSVd-lycv; Restriction Fragment Length Polymorphism analysis

References

Amari K., Canizares M. C., Myrta A.,

Sabanadzovic S., Srhiri M., Gavriel I.,

Caglayan K., Varveri C., Gatt M. and Di

Terlizzi B. (2000) First report on Hop stunt

viroid (HSVd) from some Mediterranean

countries. Phytopathologia Mediterranea

39:271-276.

2. Anirban Roy and Ramachandran P. (2003)

Occurrence of a Hop stunt viroid (HSVd)

variant in yellow corky vein disease of citrus

in India. Current Science 85:1608-1612.

3. Avina-Padilla K., de la Vega O. M., Rivera-

Bustamante R., Martinez-Soriano J. P.,

Owens R. A., Hammond R. W. and Vielle-

Calzada J.-P. (2015) In silico prediction and

validation of potential gene targets for

pospiviroid-derived small RNAs during

tomato infection. Gene 564:197-205.

4. Azad P. 1993. Reduction in yield of acid

limes infected with citrus corky vein virus in

Assam. In Proceedings of the 12th

Conference of International Organization

Citrus Virologist. IOCV, Riverside. 458.

5. Bagherian S. and Izadpanah K. 2010. Two

novel variants of hop stunt viroid associated

with yellow corky vein disease of sweet

orange and split bark disorder of sweet lime.

Pp: 105-113. In 21 st International

Conference on Citrus Virus and other Graft

Transmissible Diseases of Fruits

Crops.[Links].

6. Gorsane F., Elleuch A., Hamdi I., Salhi-

Hannachi A. and Fakhfakh H. (2010)

Molecular detection and characterization of

Hop stunt viroid sequence variants from

naturally infected pomegranate (Punica

granatum L.) in Tunisia. Phytopathologia

Mediterranea 49:152-162.

7. Jamshidi E., Jafarpour B., Rouhani H. and

Salehi M. (2014) Association of members of

clover proliferation (16SrVI) and pigeon pea

witches' broom (16SrIX) phytoplasma

groups with tomato big bud disease in Iran.

Iranian Journal of Plant Pathology 50:77-89,

Pe175.

8. Katsarou K., Rao A., Tsagris M. and

Kalantidis K. (2015) Infectious long noncoding

RNAs. Biochimie 117:37-47.

9. Roy A. and Ramachandran P. (2006)

Characterization of a Citrus exocortis viroid

variant in yellow corky vein disease of citrus

in India. Current Science (00113891) 91.

10. Su X., Fu S., Qian Y., Xu Y. and Zhou X.

(2015) Identification of Hop stunt viroid

infecting Citrus limon in China using small

RNAs deep sequencing approach. Virology

journal 12:1.