Analysis of Chalcone Synthase and Chalcone Isomerase Gene Expression in Pigment Production Pathway at Different Flower Colors of Petunia Hybrida

Journal of Cell and Molecular Research

مقاله 2، دوره 8، شماره 1 - شماره پیاپی 15، مهر 2016، صفحه 8-14   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v8i1.50406

نویسندگان

fatemeh keykha¹؛ Abdol Reza Bagheri^{* 2}؛ Nasrin Moshtaghi²

¹Department of Plant Breeding and Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. 2. Department of Plant Biotechnology, College of Agriculture, Jahrom University, Jahrom, Iran.

²Department of Plant Breeding and Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

چکیده

Variegation in flower color is commonly observed in many plant species and also occurs on petunia (Petunia hybrida)
as an ornamental plant. Variegated plants are highly valuable in the floricultural market. To gain a global perspective
on genes differentially expressed in variegated petunia flowers, we investigated the expression of chalcone synthase
(chs) and chalcone isomerase (chi) as two essential genes in biosynthesis pathway of pigment production. Also, we
measured the concentration level of total flavonoids, naringenin chalcone and naringenin to evaluate the probably
relationship between the expression profile of chs and chi genes and the concentration of mentioned pigments. The
results indicated that chalcone synthase and chalcone isomerase expression had different profile in different petal
color of Petunia hybrida. Because red flower color in petunia is related to the synthesis of pelargonidin-based (orange
to red) pigments, our results suggest that the low chalcone synthase and chalcone isomerase expression levels in
white petals reduce dihydrokaempferol formation, thereby inhibiting pelargonidin production. In contrast, the high
expression levels of these genes observed in red petals ensure sufficient anthocyanin yields to make flowers red.

کلیدواژه‌ها

Real-Time PCR؛ Chs, Chi؛ Petunia hybrida

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