Genetic diversity determination of Fusarium oxysporum f.sp. ciceri the causal agent of wilting and chlorosis in chickpea by using RAPD and PCR-RFLP techniques in Razavi and Northern Khorasan provinces

پژوهش‌های حبوبات ایران

Article 2, Volume 3, Issue 2, December 2012   

Document Type: مقالات پژوهشی

DOI: 10.22067/ijpr.v1391i2.24531

Authors

Samaneh Zokaee^* ; Mahrokh Felahati Rastegar; Behroz Jafarpour; Abdolreza Bagheri; Vahid Jahanbakhsh Mashhadi

Abstract

In order to study the genetic diversity of the causal agent of Fusarium wilting in chickpea, two techniques including RAPD and PCR-RFLP were used. In RAPD-PCR technique, all 14 primers could show the diversity among the isolates. After studding the banding patterns of 14 primers, a dendrogram based on similarity matrix was constructed. The RAPD dendrogram analysis could not separate isolates based on their geographical origins. In PCR-RFLP technique, two primers, IGS2 and CLN12, were used which made the same band pattern (2.5 kb) in all isolates. Totally, 22 polymorphic bands obtained through digestion with three digestive enzymes. The digestive enzyme RsaI produced the highest number of polymorphic bands (12 bands) and EcoRI produced the lowest number (3 bands). The genetic calculation results of the isolates showed that FOC85, FOC86 and FOC40 isolates (from Neyshabour), FOC32 (from Bojnord), FOC21 and FOC15 (from Bardaskan) and FOC11 (from Ghochan) showed the lowest genetic distance and the highest genetic similarity. The most genetic distance observed among FOC6 isolates (from Ghochan) compared to other isolates. Analysis of clusters in this method showed that there are not any specific relation between genotypic grouping and their geographical origins.

Keywords

Fusarium oxysporum f.sp; ciceri; Genetic variation; PCR-RFLP; RAPD