NetB negative Clostridium perfringens infection associated with acute necrotic enteritis in mynah (Acridotheres tristis), grey partridge (Perdix perdix) and turkey (Meleagris gallopavo)

Iranian Journal of Veterinary Science and Technology

مقاله 9، دوره 9، شماره 2 - شماره پیاپی 17، فروردین 2017، صفحه 64-69   

نوع مقاله: Case report

شناسه دیجیتال (DOI): 10.22067/veterinary.v9i2.56897

نویسندگان

Jamshid Razmyar^{* 1}؛ Massoud Rezaee²؛ Ahmad Reza Movassaghi²؛ Bahram Shojadust³

¹University of Tehran

²Ferdowsi university of Mashhad

³University of Guelph

چکیده

A non–enterotoxin (CPE)–producing Clostridium perfringens type A, associated with enteritis in a mynah (Acridotheres tristis), a grey partridge (Perdix perdix) and a turkey (Meleagris gallopavo) was characterized from cases with clinical symptoms from September 2010 until October 2012. Affected birds exhibited anorexia and diarrhea. Gross and histological findings were indicative of acute necrotic enteritis. Clostridium perfringens was isolated in bacterial cultures. Multiplex PCR for toxin profiling of the isolates revealed that the all three isolates were Clostridium perfringens type A, positive for cpb2 and cpa.

کلیدواژه‌ها

Mynah (Acridotheres tristis)؛ Grey Partridge (Perdix perdix)؛ Turkey (Meleagris gallopavo)؛ Clostridium perfringens؛ cpb2؛ cpa

مراجع

1. Slavic D, Boerlin P, Fabri M, Klotins KC, Zoethout JK, Weir PE, et al. Antimicrobial susceptibility of Clostridium perfringens isolates of bovine, chicken, porcine, and turkey origin from Ontario. Canadian journal of veterinary research = Revue canadienne de recherche veterinaire. 2011;75[2]:8997.

2. Songer JG. Clostridial enteric diseases of domestic animals. Clinical Microbiology Reviews. 1996;9[2]:216-34.

3. Van Immerseel F, De Buck J, Pasmans F, Huyghebaert G, Haesebrouck F, Ducatelle R. Clostridium perfringens in poultry: an emerging threat for animal and public health. Avian pathology : journal of the WVPA. 2004;33[6]:537-49.

4. Nowell VJ, Poppe C, Parreira VR, Jiang YF, Reid-Smith R, Prescott JF. Clostridium perfringens in retail chicken. Anaerobe. 2010;16[3]:314-5.

5. Timbermont L, Lanckriet A, Gholamiandehkordi AR, Pasmans F, Martel A, Haesebrouck F, et al. Origin of Clostridium perfringens isolates determines the ability to induce necrotic enteritis in broilers. Comparative immunology, microbiology and infectious diseases.

2009;32[6]:503-12.

6. Van Immerseel F, Rood JI, Moore RJ, Titball RW. Rethinking our understanding of the pathogenesis of necrotic enteritis in chickens. Trends in microbiology. 2009;17[1]:32-6.

7. Crespo R, Fisher DJ, Shivaprasad HL, Fernandez-Miyakawa ME, Uzal FA. Toxinotypes of Clostridium perfringens isolated from sick and healthy avian species. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 2007;19[3]:329-33.

8. Kwon YK, Lee YJ, Mo IP. An outbreak of necrotic enteritis in the ostrich farm in Korea. The Journal of veterinary medical science. 2004;66[12]:16135.

9. O’Toole D, Mills K, Ellis R, Farr R, Davis M. Clostridial enteritis in red lories [Eos bornea]. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 1993;5[1]:111-3.

10. Petit L, Gibert M, Popoff MR. Clostridium perfringens: toxinotype and genotype. Trends in microbiology. 1999;7[3]:104-10.

11. Pizarro M, Hofle U, Rodriguez-Bertos A, Gonzalez-Huecas M, Castano M. Ulcerative enteritis [quail disease] in lories. Avian diseases. 2005;49[4]:606-8.

12. Razmyar J, Kalidari GA, Tolooe A, Rad M, Movassaghi AR. Genotyping of Clostridi um perfringens isolated from healthy and diseased ostriches [Struthio camelus]. Iranian journal of microbiology. 2014;6[1]:31-6.

13. Timbermont L, Haesebrouck F, Ducatelle R, Van Immerseel F. Necrotic enteritis in broilers: an updated review on the pathogenesis. Avian pathology : journal of the WVPA. 2011;40[4]:341-7.

14. Engberg RM, Hedemann MS, Jensen BB. The influence of grinding and pelleting of feed on the microbial composition and activity in the digestive tract of broiler chickens. British poultry science. 2002;43[4]:569-79.

15. Gholamiandehkordi AR, Timbermont L, Lanckriet A, Van Den Broeck W, Pedersen K, Dewulf J, et al. Quantification of gut lesions in a subclinical necrotic enteritis model. Avian pathology : journal of the WVPA. 2007;36[5]:375-82.

16. Luna LG, Pathology AFIo. Manual of Histologic Staining Methods ; of the Armed Forces Institute of Pathology. Edited by Lee G. Luna: Blakiston Division, McGraw-Hill; 1968.

17. Coursodon CF, Glock RD, Moore KL, Cooper KK, Songer JG. TpeL-producing strains of Clostridium perfringens type A are highly virulent for broiler chicks. Anaerobe. 2012;18[1]:117-21.

18. Keyburn AL. NetB, a new toxin that is associated with avian necrotic enteritis caused by Clostridium perfringens. 2008;4[2].

19. Shivaprasad HL, Uzal F, Kokka R, Fisher DJ, McClane BA, Songer AG. Ulcerative enteritis-like disease associated with Clostridium perfringens type A in bobwhite quail [Colinus virginianus]. Avian diseases. 2008;52[4]:635-40.

20. Chalmers G, Bruce HL, Hunter DB, Parreira VR, Kulkarni RR, Jiang YF, et al. Multilocus sequence typing analysis of Clostridium perfringens isolates from necrotic enteritis outbreaks in broiler chicken populations. Journal of clinical microbiology. 2008;46[12]:3957-64.

21. Keyburn AL, Yan XX, Bannam TL, Van Immerseel F, Rood JI, Moore RJ. Association between avian necrotic enteritis and Clostridium perfringens strains expressing NetB toxin. Veterinary research. 2010;41[2]:21.

22. Martin TG, Smyth JA. Prevalence of netB among some clinical isolates of Clostridium perfringens from animals in the United States. Veterinary microbiology. 2009;136[12]:202-5.

23. Salari Sedigh H, Razmyar J and Ghavidel GH. Isolation and genotyping of Clostridium perfringens from healthy and diarrheic dogs. Iranian Journal of Veterinary Science and Technology. 2016; 8[2]: 48-54