Prefractionation in Proteome Profile Analysis of ANXC4 Gene Mutant in Aspergillus Fumigatus

Journal of Cell and Molecular Research

مقاله 5، دوره 10، شماره 2 - شماره پیاپی 20، خرداد 2019، صفحه 90-96   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/jcmr.v10i2.73401

نویسندگان

Elham Erami^{* 1}؛ Fereydoun Sadeq-zadeh²

¹Pasteur Institute of Iran, Tehran, Iran

²Green Environment and Sustainable Chemistry, Tehran, Iran

چکیده

     Aspergillus fumigatus is one of the pathogenic filamentous fungi that could cause opportunistic infection, allergy and poisoning. Since, genetic sequencing of some of these fungi has been completed, identifying the protein profile of these fungus cells is necessary. One of these genes is the Annexin family. Annexin C4 is a new member of fungal annexins. This study investigates the effect of ANXC4 mutant gene in proteome profile of Aspergillus Fumigatus. Moreover, in order to enhance the power of protein complex separation, we used an optimized prefractionation method. Using reverse phase-high performance liquid chromatography eight fractions were separated. Then to confirm the protein concentration, each fraction was tested by SDS-PAGE. Protein profile of these fractions was analyzed using 2-DE, and Image Master software. Among the proteins identified statistically, two emerging proteins were observed. The results show that the expression of ANXC4 could affect the expression of some proteins in A.Fumigatus. To accurately identify these proteins, further experiments are needed including Mass spectrometry analysis.

کلیدواژه‌ها

Aspergillus Fumigatus؛ RP-HPLC؛ 2-DE؛ prefractionation؛ proteome profile

مراجع

Anderson, N. L., Matheson, A. D. and Steiner, S. (2000) Proteomics: Applications in basic and applied biology. Current Opinion in Biotechnology, pp. 408–412.

Badock, V. Steinhvsen,V.,Bommert,K.,Otto,A. (2001) Prefractionation of protein samples for proteome analysis using reversed-phase high-performance liquid chromatography. Electrophoresis, 22(14), pp. 2856–2864.

Dagenais, T. R. T. and Keller, N. P. (2009) Pathogenesis of Aspergillus fumigatus in invasive aspergillosis. Clinical Microbiology Reviews. American Society for Microbiology (ASM), pp. 447–465.

Debeaupuis, J. P. Sarfati,J.,Chazlet,V.,and paullatge,.J.(1997) Genetic diversity among clinical and environmental isolates of Aspergillus fumigatus. Infection and immunity, 65(8), pp. 3080–5.

Gerke, V. and Moss, S. E. (2002) Annexins: From Structure to Function. Physiological Reviews, 82(2), pp. 331–371.

Govorun, V. M. and Archakov, A. I. (2002) Proteomic technologies in modern biomedical science. Biochemistry (Moscow), pp. 1109–1123.

Karpievitch, Y. V ,.polpitiya,A.D.,Anderson,G.D.,Smith,R.D.,andDabney,A.R. (2010) Liquid chromatography mass spectrometry-based proteomics: Biological and technological aspects. Annals of Applied Statistics, 4(4), pp. 1797–1823.

Khalaj, K., Aminollahi, E., Bordbar, A., and Khalaj, V., Fungal annexins: a mini review (2015)

Khalaj, V. Smith,L.,Brookman,J.,Tuckwell,D. (2004) Identification of a novel class of annexin genes. FEBS Letters, 562(1–3), pp. 79–86.

Khalaj, V. Azarian,B.,Enayati,S.,Vaziri,B. (2011)Annexin C4 in A. Fumigatus: A proteomics approach to understand the function. Journal of Proteomics, 74(10), pp. 1950–1958.

Latge, J. P. (2001) The pathobiology of Aspergillus fumigatus. Trends in Microbiology, pp. 382–389.

Maryam, M., FU, M, S., Alaiano, A., Camacho, E., Diego, G., Faneuff, E. E. (2019)The enigmatic role of fungal annexins: the case of cryptococcus neoformans

Moss, S. E. and Morgan, R. O. (2004) The annexins. Genome Biology. BioMed Central, p. 219.

Neverova, I. and Van Eyk, J. E. (2005) Role of chromatographic techniques in proteomic analysis. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, pp. 51–63.

Righetti, P. G. Castagna, A.Herbert,B. Reymond,F.Rossier,J.S.(2003) Prefractionation techniques in proteome analysis. in Proteomics, pp. 1397–1407.

Righetti, P. G. Castagna, A.AntonioliL, P.(2005) Prefractionation techniques in proteome analysis: The mining tools of the third millennium. Electrophoresis, pp. 297–319.

Ronning, C. M. Fedorova, N.D., Bowyer, P., Coulson, R., Goldman, G., Kim, H.S., Turner, G., Wortman, J.R., Yu, J., Anderson, M.J., Denning, D.W., Nierman, W.C. (2005)Genomics of Aspergillus fumigatus. Revista Iberoamericana de Micologia, pp. 223–228.

Srinivas, P. R., Verma , M.,Zhao , Y., and Srvastava, S. (2002) Proteomics for cancer biomarker discovery. Clinical Chemistry, pp. 1160–1169.

Van Eyk, J. E. (2001) Proteomics: unraveling the complexity of heart disease and striving to change cardiology. Curr Opin Mol Ther, 3(6), pp. 546–553.

Vaziri, B. ,Rahimpour,M.,Eslami,N.,Fayaz,A.,Rahimian,H. (2006) RP-HPLC prefractionation and its application in expressional proteomics analysis of an in vitro viral infection model. Journal of Separation Science, 29(15), pp. 2284–2291.