Molecular epidemiology of Campylobacter Fetus in aborted fetuses of Baluchi sheep in Sistan region

Iranian Journal of Veterinary Science and Technology

مقاله 7، دوره 10، شماره 1 - شماره پیاپی 18، دی 2018، صفحه 47-52   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v10i1.69012

نویسندگان

Ebrahim Hossein Abadi¹؛ Dariush Saadati^{* 2}؛ Mohsen Najimi²؛ Mehdi Hassanpour³

¹university of Tehran

²University of Zabol

³University of Tehran

چکیده

Campylobacter is one of the main bacterial causes of ewe abortion throughout the world. Campylobacter infections are now considered as zoonoses. The objective of this study was an investigation of Campylobacter fetus prevalence among aborted ovine fetuses in the Sistan region (north of Sistan and Baluchestan province). In the present study, spleen and abomasum content samples were obtained from 78 aborted lambs of Baluchi sheep. The samples were examined for campylobacter contamination using PCR method. The overall prevalence of campylobacter infection was 7.7%. The prevalence of infection in fetuses aged three months and under were significantly higher than that in fetuses older than three months. The result of this study showed that C. fetus should be considered as one of the infectious causes of abortion among sheep flocks in Sistan region.

کلیدواژه‌ها

abortion؛ Campylobacter؛ Sheep؛ Sistan؛ PCR

مراجع

West D. Ovine abortion in New Zealand. New Zealand veterinary journal. 2002;50(3):93-5.

Sahin O, Plummer PJ, Jordan DM, Sulaj K, Pereira S, Robbe-Austerman S, et al. Emergence of a tetracycline-resistant Campylobacter jejuni clone associated with outbreaks of ovine abortion in the United States. Journal of clinical Microbiology. 2008;46(5):1663-71.

Hamali H, Fallah S, Joozani RJ, Zare P, Noorsaadat G. Detection of Campylobacter spp. in sheep aborted fetuses by PCR. Trends Life Sci. 2014;3:49-56.

Firouzi R. Bacteriological Study of Abortion in Ewes of Shiraz Area. Journal of Veterinary Research. 2006;61(1):15-7.

Ghousian MM, Keyvani AH, Zahraei SM, Khazraeinia P, Falah N. A comparison of culture and PCR methods wrights test for diagnosis of brucellosis. Daneshvarmed. 2008;15(5):51-8.

Mahzounieh M, Golbuy Daghdari S, Pourahmad R. Detection of Chlamydophila abortus in sheep abortions in Chaharmahal va Bakhtiari Province using Nested PCR. Iranian veterinary journal. 2014;10(2):74-80.

Wagenaar JA, van Bergen MA, Blaser MJ, Tauxe RV, Newell DG, van Putten JP. Campylobacter fetus infections in humans: exposure and disease. Clinical Infectious Diseases. 2014;58(11):1579-86.

Mearns R. Other infectious causes of abortion. In: Aitken I, editor. Diseases of Sheep. Fourth Edition ed. Edinburgh: Blackwell; 2007. p. 127-37.

Hum S, Quinn K, Brunner J. Evaluation of a PCR assay for identification and differentiation of Campylobacter fetus subspecies. Australian Veterinary Journal. 1997;75(11):827-31.

Salihu M, Junaidu A, Oboegbulem S, Egwu G. Prevalence and biotypes of Campylobacter species isolated from sheep in Sokoto State, Nigeria. International Journal of Animal and Veterinary Advances. 2009;1(1):6-9.

Fenwick S, West D, Hunter J, Sargison N, Ahmed F, Lumsden J, et al. Campylobacter fetus fetus abortions in vaccinated ewes. New Zealand veterinary journal. 2000;48(5):155-7.

Mannering S, West D, Fenwick S, Marchant R, O’Connell K. Pulsed-field gel electrophoresis of Campylobacter jejuni sheep abortion isolates. Veterinary microbiology. 2006;115(1):237-42.

Erganis O, Kaya O, Hadimli H, Guler L. Rapid diagnosis of ovine Brucella, Campylobacter and Salmonella infections from fetal stomach contents by coagglutination test. Small Ruminant Research. 2002;45(2):123-7.

Wolffs P, Norling B, Hoorfar J, Griffiths M, Rådström P. Quantification of Campylobacter spp. in chicken rinse samples by using flotation prior to real-time PCR. Applied and environmental microbiology. 2005;71(10):5759-64.

Gharekhani J, Karimi Makhsoos A, Sadeghi B. Study of bacterial causes of abortion in sheep in Hamedan province. Journal of Large Animal Clinical Science Research. 2011;5 (3):9-14.

Tuzcu M, Oruc E, Tuzcu N, Yoldaș A, Yığın A. Diagnosis of campylobacteriosis in the aborted bovine foetuses by pathological, immunohistochemical, microbiological and real time PCR. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010;16(3):509-14.

Agerholm JS, Aalbæk B, FOG‐LARSEN A, Boye M, Holm E, Jensen TK, et al. Veterinary and medical aspects of abortion in Danish sheep. Apmis. 2006;114(2):146-52.

Bird M, Stephens D, Wall E. Serology of Campylobacter fetus fetus strains from four outbreaks of ovine abortion. New Zealand veterinary journal. 1984;32(1-1):14-7.

Firehammer B, Lovelace S. The Isolation of V. bubulous (Florent) from sheep. Am J Vet Res. 1962;22:449-60.

Givens MD, Marley M. Infectious causes of embryonic and fetal mortality. Theriogenology. 2008;70(3):270-85.

Saleh M, Harkinezhad MT, Marefat A, Salmani V. An outbreak of abortion in Afshari sheep with probable involvement of Campylobacter Fetus. Iran J Vet Med. 2013;7(1):51–6.