Experimental infection of pheasants with a velogenic chicken isolate of Newcastle disease virus

Iranian Journal of Veterinary Science and Technology

مقاله 2، دوره 11، شماره 1 - شماره پیاپی 20، اسفند 2019، صفحه 7-12   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v1i11.80546

نویسندگان

Ramezan Ali Jafari^{* 1}؛ Anahita Rezaie²؛ Zahra Boroomand³؛ Mansoor Mayahi³؛ Reza Zare³

¹Shahid Chamran University of Ahvaz

²Shahid Chamran University of

³Shahid Chamran University

چکیده

Newcastle disease (ND) is a highly contagious infection of many avian species, causing enormous losses in poultry production worldwide. The objective of this study was to reveal the clinical feature, virus shedding, and immune response following infection with a velogenic chicken isolate of Newcastle disease virus (NDV) in susceptible and vaccinated pheasants. Eighty day-old pheasant chicks were allotted to four groups. At 30 days of age, the birds in groups 1 and 3 were vaccinated with B1 strain via eye drop. Two weeks later, each bird in groups 1 and 2 was inoculated with 100 μL(50 μL/eye) of NDV-infected allantoic fluid containing 105 EID50 of viral inoculum. All groups were inspected daily for three weeks. Swab samples were taken at different time points and verifi ed for NDV infection by using reverse-transcription polymerase chain reaction (RT-PCR). Serological examination was also made by haemagglutination-inhibition assay. Clinically, watery mucoid feces was observed only in one case among the vaccinated challenged birds, whereas the unvaccinated challenged birds showed anorexia, mild depression and head deviation. Out of 20 birds in group 2, one case (5%) died. Based on RT-PCR, virus shedding was only observed among the unvaccinated birds from 5 to 14 days aft er challenge. The NDV was detected more in tracheal swabs (40%) than in cloacal swabs (30%). The infected birds showed a high seroconversion. In conclusion, the velogenic NDV circulating in Iranian chicken flocks has a low pathogenicity for pheasants, and ocular vaccination with B1 strain could provide a good protection.

کلیدواژه‌ها

Immune response؛ Newcastle Disease؛ Pheasant؛ Virus shedding

مراجع

1. Kaleta EF, Baldauf C. Newcastle disease in free living and pet

birds. In: Alexander DJ, editor. Newcastle

disease. Netherlands: Kluwer

Academic Publishers; 1988.

2. World Organization for Animal Health

(OIE). Newcastle disease. In: Manual

of diagnostic tests and vaccines

for terrestrial animals. 6th ed. OIE,

France; 2008.

3. Miller PJ, Koch G. Newcastle disease.

In: Swayne DE, Glisson JR, McDougald

LR, Nolan LK, Saurez DL, Nair

V, editors. Diseases of poultry. 13th

ed. Iowa: Blackwell publishing;2013.

4. Gale C, McCartiney C, Sanger C. Newcastle

disease in turkeys. J Am Vet

Med Assoc. 1961;139:462-5.

5. Gowthaman V, Singh SD, Barathidasan

R, Ayanur A, Dhama K. Natural outbreak

of Newcastle disease in turkeys

and Japanese quails housed along

with chicken in a multi–species poultry

farm in northern India. Adv Anim

Vet Sci. 2013;1(3S):17-20.

6. Borland ED. Newcastle disease in

pheasants, partridges and wild birds

in East Anglia, 1970-1971. Vet Rec.

1972;90(17):481-2.

7. Jaafi r NAH, Hatem HK, Faiza IZ,

Ahalm AH. An outbreak of Newcastle

disease in a pheasant fl ock in Iraq.

Avian Pathol. 1980;9(4):583-5.

8. Alexander DJ, Manvell RJ, Frost KM,

Pollitt WJ, Welchman D, Perry K.

Newcastle disease outbreak in pheasants

in Great Britain in May 1996. Vet

Rec. 1997;140(1):20-2.

9. Jorgensen PH, Handberg KJ, Ahrens P,

Hansen HC, Manvell RJ, Alexander

DJ. An outbreak of Newcastle disease

in free-living pheasants (Phasianus

colchicus). J Vet Med Series B.

1999;46(6):381-7.

10. Aldous EW, Manvell RJ, Cox WJ,

Ceeraz V, Harwood D, Shell W, et al.

An outbreak of Newcastle disease in

pheasants (Phasianus colchicus) in

south-east England in July 2005. Vet

Rec. 2007;160(14):482-4.

11. Momayez R, Gharahkhani P, Pourbaksh

SA, Toroghi R, Shoushtari AH,

Banai M. Isolation and pathogenicity

identifi cation of avian paramyxovirus

serotype 1 (Newcastle disease) virus

from a Japanese quail fl ock in Iran.

Arch Razi Inst. 2007;62(1):39-44.

12. Ghiamirad M, Pourbakhsh A, Keyvanfar

H, Momayaz R, Charkhkar S,

Ashtari A. Isolation and characterization

of Newcastle disease virus from

ostriches in Iran. Afr J Microbiol Res.

2010;4(23):2492-7.

13. Madadgar O, Karimi V, Nazaktabar

A, Kazemimanesh M, Ghafari MM,

Azimi Dezfouli SM. A study of Newcastle

disease virus obtained from

exotic caged birds in Tehran between

2009 and 2010. Avian Pathol.

2013;42(1):27-31.

14. Mehrabanpour MJ, Khoobyar S, Rahimian

A, Nazari MB, Keshtkar MR.

Phylogenetic characterization of the

fusion genes of the Newcastle disease

viruses isolated in Fars province poultry

farms during 2009-2011. Vet Res

Forum. 2014;5(5):187-91.

15. Boroomand Z, Jafari RA, Mayahi M.

Molecular characterization and phylogenetic

study of the fusion genes of

Newcastle disease virus from the recent

outbreaks in Ahvaz, Iran. Virusdisease.

2016;27(1):102-5.

16. Wakamatsu N, King DJ, Kapczynski

DR, Seal BS, Brown CC. Experimental

pathogenesis for chickens, turkeys,

and pigeons of exotic Newcastle disease

virus from an outbreak in California

during 2002-2003. Vet Pathol.

2006;43(6):925-33.

17. Capua I, Dalla PM, Multinelli F, Marangon

S, Terregino C. Newcastle disease

outbreaks in Italy during 2000.

Vet Rec. 2002;150(18):565-8.

18. Schmidt EMDS, Paulillo AC, Filho TF,

Agustini M, Ventura HLB, Junior LD.

Clinical and immunological parameters

of Newcastle disease vaccination

in juvenile ring-neck pheasants. Int J

Poult Sci. 2008;7(3):283-4.

19. Kinde H, Hullinger PJ, Charlton B,

McFarland M, Hietala SK, Velez V, et

al. Th e isolation of exotic Newcastle

disease (END) virus from nonpoultry

avian species associated with the epidemic

of END in chickens in southern

California: 2002–2003. Avian Dis.

2005;49(2):195-8.

20. Jafari RA, Boroomand Z, Rezaie A,

Mayahi M, Nejati Saravi A. Experimental

infection of turkeys with a virulent

Newcastle disease virus isolated

from broiler chickens. Arch Razi Instit.

2019;74(1):51-7.

21. Haque MH, Hossain MT, Islam MT,

Zinnah MA, Khan MSR, Islam MA.

Isolation and detection of Newcastle

disease virus from fi eld outbreaks in

broiler and layer chickens by reverse

transcription-polymerase chain reaction.

Bangl J Vet Med. 2010;8(2):87-

92.

22. Perozo F, Villegas P, Dolz R, Afonso

CL, Purvis LB. Th e VG/GA strain of

Newcastle disease virus: mucosal immunity,

protection against lethal challenge

and molecular analysis. Avian

Pathol. 2008;37(3):237-45.

23. Piacenti AM, King DJ, Seal BS, Zhang

J, Brown CC. Pathogenesis of Newcastle

disease in commercial and specifi c

pathogen-free turkeys experimentally

infected with isolates of diff erent virulence.

Vet Pathol. 2006;43(2):168-78.

24. Alexander DJ, Senne DA. Newcastle

disease. In: Saif YM, Fadly AM, Glisson

JR, McDougald LR, Nolan NK,

Swayne DE, editors. Diseases of poultry.

12th ed. Iowa: Blackwell publishing;

2008.

25. Villegas P. Titration of biologicacal

suspensions. In: Dufor-Zavala L,

Swayne DE, Glison JR, Pearson JE,

Reed MW, Woolcock PR, editors. A

laboratory manual for the isolation,

identifi cation and characterization of

avian pathogens. 5th ed. Athene, GA:

American Association of Avian Pathologist;

2008.

26. Th ayer SG, Beard CW. Serologic procedure.

In: Dufor-Zavala L, Swayne

DE, Glison JR, Pearson JE, Reed MW,

Woolcock PR, editors. A laboratory

manual for the isolation, identifi -

cation and characterization of avian

pathogens. 5th ed. Athene, GA:

American Association of Avian Pathologist;

2008.

27. Petrie A, Watson, P. Statistics for Veterinary

and Animal Science. 2nd ed.

Iowa: Blackwell Publishing; 2006.