The Eff ect of resistance training and growth hormone injection on circulating IGF-1 and IGFBP-3 levels in a rat model

Iranian Journal of Veterinary Science and Technology

مقاله 3، دوره 11، شماره 1 - شماره پیاپی 20، اسفند 2019، صفحه 13-18   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v1i11.78504

نویسندگان

Behnam Roozbeh؛ Mahtab Moazami^* ؛ Amir Rashidlamir؛ Zahra Moosavi؛ Ali Javadmanesh

Ferdowsi University of Mashhad

چکیده

Growth hormone has mitotic and anti-apoptotic effects which may increase proliferation and transformation of cells when it is expressed aberrantly. This study investigated the effects of resistance training and growth hormone injection on circulating IGF-1, IGFBP-3 levels and IGF-1/IGFBP-3 ratio in male Wistar rats. Thirty-two male Wistar rats were randomly assigned to a control group (C, n = 8), a resistance training group (RT, n = 8), a growth hormone injection group (GI, n = 8) and a resistance training + growth hormone injection group (RG, n = 8). The resistance training protocol comprised of climbing a ladder (5 days/week, 3 sets/5 reps) while carrying a weight suspended from the tail. The growth hormone (2 mg/kg/day, 5 days/week) was injected before an exercise session. Serum IGF-1, IGFBP-3 levels, and IGF-1/IGFBP-3 ratio were measured after 8 weeks. One-way ANOVA analysis was used for comparison of serum IGF-1 and IGFBP-3 levels between groups. Serum IGF-1 levels and IGF-1/IGFBP-3 ratio signifi cantly decreased, but serum IGFBP-3 levels showed no significant change in the RT group compared to the C group. Also, both serum IGF-1 and IGFBP-3 levels and IGF-1/IGFBP-3 ratio in GI and RG groups significantly increased compared to the other groups. In conclusion, resistance training decreases serum IGF-1 levels and/or IGF-1/IGFBP-3 ratio in normal condition. On the other hand, the growth hormone injection with and without the resistance training increases serum IGF-1 levels and IGF-1/IGFBP-3 ratio which could be noted as a condition with a higher risk of neoplasm.

کلیدواژه‌ها

Resistance training؛ Growth hormone injection؛ IGF-1؛ IGFBP-3؛ cancer

مراجع

1. Bird SR, Goebel C, Burke LM, Greaves RF. Doping in sport

and exercise: anabolic, ergogenic, health and clinical issues.

Ann Clin Biochem. 2016;53(Pt 2):196-221.

2. Hermansen K, Bengtsen M, Kjaer M, Vestergaard P, Jorgensen

JOL. Impact of GH administration on athletic performance

in healthy young adults: A systematic review and

meta-analysis of placebo-controlled trials. Growth Horm IGF

Res. 2017;34:38-44.

3. Ghanipoor-Samami M, Javadmanesh A, Burns BM, Th omsen

DA, Nattrass GS, Estrella CAS, et al. Atlas of tissue- and

developmental stage specifi c gene expression for the bovine

insulin-like growth factor (IGF) system. PLoS One.

2018;13(7):e0200466.

4. Jones JI, Clemmons DR. Insulin-like growth factors and

their binding proteins: biological actions. Endocr Rev.

1995;16(1):3-34.

5. Denduluri SK, Idowu O, Wang Z, Liao Z, Yan Z, Mohammed

MK, et al. Insulin-like growth factor (IGF) signaling in tumorigenesis

and the development of cancer drug resistance.

Genes Dis. 2015;2(1):13-25.

6. Majorczyk M, Smolag D. Eff ect of physical activity on IGF-1

and IGFBP levels in the context of civilization diseases prevention.

Rocz Panstw Zakl Hig. 2016;67(2):105-11.

7. Allen NE, Key TJ. Re: plasma insulin-like growth factor-I, insulin-

like growth factor-binding proteins, and prostate cancer

risk: a prospective study. J Natl Cancer Inst. 2001;93(8):649-

51.

8. Chan JM, Stampfer MJ, Giovannucci E, Gann PH, Ma

J, Wilkinson P, et al. Plasma insulin-like growth factor-I

and prostate cancer risk: a prospective study. Science.

1998;279(5350):563-6.

9. Endogenous H, Breast Cancer Collaborative G, Key TJ, Appleby

PN, Reeves GK, Roddam AW. Insulin-like growth factor

1 (IGF1), IGF binding protein 3 (IGFBP3), and breast

cancer risk: pooled individual data analysis of 17 prospective

studies. Lancet Oncol. 2010;11(6):530-42.

10. Hankinson SE, Willett WC, Colditz GA, Hunter DJ, Michaud

DS, Deroo B, et al. Circulating concentrations of insulin-

like growth factor-I and risk of breast cancer. Lancet.

1998;351(9113):1393-6.

11. Singh P, Rubin N. Insulinlike growth factors and binding proteins

in colon cancer. Gastroenterology. 1993;105(4):1218-37.

12. Stattin P, Rinaldi S, Biessy C, Stenman UH, Hallmans G,

Kaaks R. High levels of circulating insulin-like growth factor-

I increase prostate cancer risk: a prospective study in

a population-based nonscreened cohort. J Clin Oncol.

2004;22(15):3104-12.

13. Johansson H, Baglietto L, Guerrieri-Gonzaga A, Bonanni B,

Mariette F, Macis D, et al. Factors associated with circulating

levels of insulin-like growth factor-I and insulin-like growth

factor binding protein-3 in 740 women at risk for breast cancer.

Breast Cancer Res Treat. 2004;88(1):63-73.

14. Yu H, Spitz MR, Mistry J, Gu J, Hong WK, Wu X. Plasma

levels of insulin-like growth factor-I and lung cancer risk: a

case-control analysis. J Natl Cancer Inst. 1999;91(2):151-6.

15. Zhao H, Grossman HB, Spitz MR, Lerner SP, Zhang K, Wu X.

Plasma levels of insulin-like growth factor-1 and binding protein-

3, and their association with bladder cancer risk. J Urol.

2003;169(2):714-7.

16. Ma J, Pollak MN, Giovannucci E, Chan JM, Tao Y, Hennekens

CH, et al. Prospective study of colorectal cancer risk in men

and plasma levels of insulin-like growth factor (IGF)-I and

18 IGF-binding protein-3. J Natl Cancer

Inst. 1999;91(7):620-5.

17. Renehan AG, O’Dwyer ST, Shalet SM.

RESPONSE: more about: prospective

study of colorectal cancer risk in

men and plasma levels of insulin-like

growth factor (IGF)-I and IGF-binding

protein-3. J Natl Cancer Inst.

2000;92(23):1949.

18. Koziris LP, Hickson RC, Chatterton

RT, Jr., Groseth RT, Christie JM,

Goldfl ies DG, et al. Serum levels of

total and free IGF-I and IGFBP-3 are

increased and maintained in longterm

training. J Appl Physiol (1985).

1999;86(4):1436-42.

19. Kraemer WJ, Aguilera BA, Terada M,

Newton RU, Lynch JM, Rosendaal G,

et al. Responses of IGF-I to endogenous

increases in growth hormone

aft er heavy-resistance exercise. J Appl

Physiol (1985). 1995;79(4):1310-5.

20. Kraemer WJ, Hakkinen K, Newton

RU, Nindl BC, Volek JS, McCormick

M, et al. Eff ects of heavy-resistance

training on hormonal response patterns

in younger vs. older men. J Appl

Physiol (1985). 1999;87(3):982-92.

21. Kraemer WJ, Marchitelli L, Gordon

SE, Harman E, Dziados JE, Mello R,

et al. Hormonal and growth factor

responses to heavy resistance exercise

protocols. J Appl Physiol (1985).

1990;69(4):1442-50.

22. Rosendal L, Langberg H, Flyvbjerg A,

Frystyk J, Orskov H, Kjaer M. Physical

capacity infl uences the response of insulin-

like growth factor and its binding

proteins to training. J Appl Physiol

(1985). 2002;93(5):1669-75.

23. Schwarz AJ, Brasel JA, Hintz RL, Mohan

S, Cooper DM. Acute eff ect of

brief low- and high-intensity exercise

on circulating insulin-like growth

factor (IGF) I, II, and IGF-binding

protein-3 and its proteolysis in young

healthy men. J Clin Endocrinol Metab.

1996;81(10):3492-7.

24. Eliakim A, Brasel JA, Mohan S,

Wong WL, Cooper DM. Increased

physical activity and the growth hormone-

IGF-I axis in adolescent males.

Am J Physiol. 1998;275(1 Pt 2):R308-

14.

25. Bang P, Brandt J, Degerblad M, Enberg

G, Kaijser L, Th oren M, et al. Exercise-

induced changes in insulin-like

growth factors and their low molecular

weight binding protein in healthy

subjects and patients with growth

hormone defi ciency. Eur J Clin Invest.

1990;20(3):285-92.

26. Mannerkorpi K, Landin-Wilhelmsen

K, Larsson A, Cider A, Arodell O,

Bjersing JL. Acute eff ects of physical

exercise on the serum insulin-like

growth factor system in women with

fi bromyalgia. BMC Musculoskelet

Disord. 2017;18(1):37.

27. Borst SE, De Hoyos DV, Garzarella

L, Vincent K, Pollock BH, Lowenthal

DT, et al. Eff ects of resistance training

on insulin-like growth factor-I and

IGF binding proteins. Med Sci Sports

Exerc. 2001;33(4):648-53.

28. Michael V. Vitiello, Charles W.

Wilkinson, George R. Merriam, Karen

E. Moe, Patricia N. Prinz, David

D. Ralph, et al. Successful 6-Month

Endurance Training Does Not Alter

Insulin-Like Growth Factor-I

in Healthy Older Men and Women.

Th e Journals of Gerontology.

1997;52A(3):M149-M54.

29. Redd MJ, Hoff man JR, Gepner Y,

Stout JR, Hoff man MW, Ben-Dov D,

et al. Th e eff ect of HMB ingestion on

the IGF-I and IGF binding protein

response to high intensity military

training. Growth Horm IGF Res.

2017;32:55-9.

30. Nishida Y, Matsubara T, Tobina T,

Shindo M, Tokuyama K, Tanaka K, et

al. Eff ect of low-intensity aerobic exercise

on insulin-like growth factor-I

and insulin-like growth factor-binding

proteins in healthy men. Int J Endocrinol.

2010;2010.

31. Chicharro JL, Lopez-Calderon A,

Hoyos J, Martin-Velasco AI, Villa G,

Villanua MA, et al. Eff ects of an endurance

cycling competition on resting

serum insulin-like growth factor

I (IGF-I) and its binding proteins IGFBP-

1 and IGFBP-3. Br J Sports Med.

2001;35(5):303-7.

32. Nindl BC, Pierce JR. Insulin-like

growth factor I as a biomarker of

health, fi tness, and training status.

Med Sci Sports Exerc. 2010;42(1):39-

49.

33. Eliakim A, Moromisato M, Moromisato

D, Brasel JA, Roberts C, Jr., Cooper

DM. Increase in muscle IGF-I protein

but not IGF-I mRNA aft er 5 days of

endurance training in young rats. Am

J Physiol. 1997;273(4 Pt 2):R1557-61.

34. Nishijima T, Piriz J, Dufl ot S, Fernandez

AM, Gaitan G, Gomez-Pinedo U,

et al. Neuronal activity drives localized

blood-brain-barrier transport of serum

insulin-like growth factor-I into

the CNS. Neuron. 2010;67(5):834-46.

35. Sachdev D, Yee D. Disrupting insulin-

like growth factor signaling as a

potential cancer therapy. Mol Cancer

Th er. 2007;6(1):1-12.

36. Collett-Solberg PF, Cohen P. Th e

role of the insulin-like growth factor

binding proteins and the IGFBP

proteases in modulating IGF action.

Endocrinol Metab Clin North Am.

1996;25(3):591-614.

37. Kemmler W, Wildt L, Engelke K, Pintag

R, Pavel M, Bracher B, et al. Acute

hormonal responses of a high impact

physical exercise session in early

postmenopausal women. Eur J Appl

Physiol. 2003;90(1-2):199-209.

38. Nindl BC, Kraemer WJ, Marx JO, Arciero

PJ, Dohi K, Kellogg MD, et al.

Overnight responses of the circulating

IGF-I system aft er acute, heavy-resistance

exercise. J Appl Physiol (1985).

2001;90(4):1319-26.

39. Sandhu MS, Dunger DB, Giovannucci

EL. Insulin, insulin-like growth

factor-I (IGF-I), IGF binding proteins,

their biologic interactions, and

colorectal cancer. J Natl Cancer Inst.

2002;94(13):972-80.

40. Lee S, Farrar PR. Resistance training

induces muscle-specifi c changes

in muscle mass and function in

Rat. Journal of Exercise Physiology.

2003;6(2).