The eff ects of extenders containing proline and glutamine on oxidative stress and motion parameters of stallion semen during cold storage

Iranian Journal of Veterinary Science and Technology

مقاله 8، دوره 11، شماره 1 - شماره پیاپی 20، مهر 2019، صفحه 51-59   

نوع مقاله: Research Articles

شناسه دیجیتال (DOI): 10.22067/veterinary.v11i1.80929

نویسندگان

Najmeh Davoodian^* ؛ Ali Kadivar؛ Ebrahim Ahmadi

Shahrekord University of Shahrekord

چکیده

This study examined the effects of skim-milk based extenders supplemented with proline and glutamine on motility, lipid peroxidation and enzymatic antioxidant status of cooled-stored equine sperm and determined the role of seminal plasma as well. The semen was collected with artificial vagina. In experiment 1, native semen was diluted in skim-milk based extender containing 5mM glutamine and 3mM proline, stored at 5°C and analyzed at 4, 24 and 48 hours storage for motion parameters. In experiment 2, semen was centrifugated, sperm pellet resuspended in the extenders and stored at 5°C for 4 hours to determine motion parameters. The level of catalase, glutathione peroxidase activity and malondialdehyde formation was determined for all samples at 4 hours. Glutamine and proline signifi cantly preserved the percentage of motile sperm (76.5 ± 2.7 and 79.4 ± 1 vs 69 ± 1.4), increased the progressive motility of cold-stored semen (66.1 ± 2.5 and 73.7 ± 2.9 vs 56.2 ± 1.4), increased catalase activity, and decreased malondialdehyde. However these effects were disappeared after seminal plasma removal. We conclude that glutamine and proline would amplify the antioxidant activity of equine cold-stored semen and preserve its motility. This effect seems to be related to interactions with seminal plasma.

کلیدواژه‌ها

Glutamine؛ Proline؛ Cold storage؛ stallion semen؛ Antioxidant activity

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