Molecular detection of mouse hepatitis virus in laboratory mouse colonies

Iranian Journal of Veterinary Science and Technology

مقاله 8، دوره 11، شماره 2 - شماره پیاپی 21، شهریور 2019، صفحه 69-72   

نوع مقاله: Short communication

شناسه دیجیتال (DOI): 10.22067/veterinary.v11i2.77631

نویسندگان

Roozbeh Fallahi^{* 1}؛ Fatemeh Abedini²؛ Gholam Reza Shokri³

¹-Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

²Department of Research, Breeding and Production of Laboratory Animals, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.

³Research and Development Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran

چکیده

The animal health monitoring is required to issue health certificates. The viral hepatitis virus is one of the most important infectious agents in mice breeding colonies. This research used RT-PCR to identify contaminations to mouse hepatitis virus. 18 out of 29 specimens were found to be infected, a prevalence of 62%. PCR product was purified and sequenced. Phylogenetic analysis revealed that the identified strain in this study was closely related to a strain reported from France. In the conventional system, contamination with different infectious agents is inevitable, thus it is better to replace the contaminated colonies with clean animals.

کلیدواژه‌ها

Molecular detection؛ mouse؛ Hepatitis virus

مراجع