Study and Comparison Jar and Periodical Bioreactor for Mass Propagation of Rootstocks GF677 (Prunus amygdalus×Prunus persica)

علوم باغبانی

Article 5, Volume 27, Issue 1, May 2013, Pages 36-43   

Document Type: Research Article

DOI: 10.22067/jhorts4.v0i0.20784

Authors

Sakineh Bagheri^* ; Mohammad Esmaeil Amiri; Dariush Davoodi; Mehrnaz Entesari

Abstract

The GF677 (Prunus amygdalus×Prunus persica) is a peach rootstock tolerant to Fe deficiency .Therefore, mass propagation of GF677 rootstock is recommended by bioreactor. On the other hand, plant micro-propagation using bioreactors can decrease the cost of production and makes it an economical method. In this research, the possibility of using bioreactor and optimization of the tissue culture procedure in GF677 rootstock micro-propagation was studied. In vitro stock plantlets from axillary buds and liquid modify MS basal medium supplemented with 1 mg/L BAP, 0.2 mg/L GA3, and 3% sucrose were used for inoculation and multiplication in the bioreactors. Periodical nutrition of the explants established for 10 minutes in each 24 hours. Bioreactor system was compared with routine in vitro procedure in tissue culture laboratory. Results showed that the bioreactor system multiplication rate, number of shoots, and biomass production was significantly different from routine in vitro culture. The total number of shoots in bioreactor was about 600 whit 7.43 cm length and was very more efficient than routine system (20 shoots whit 2.47 cm length per 300 ml jar).

Keywords

GF677 (Prunus amygdalus×Prunus persica); Mass propagation; Periodical; Bioreactor