The Study of Culture Media Composition and Cytokinin Type Effects on Establishment and Proliferation of Walnut Explants, Eenotype Z60

علوم باغبانی

Article 9, Volume 27, Issue 1, May 2013, Pages 67-73   

Document Type: Research Article

DOI: 10.22067/jhorts4.v0i0.20792

Authors

Shadi Mohamadi-Nejad^* ; M. Gholami; Mahmood Esna-Ashari

Abstract

This research was conducted to study the factors affecting establishment, growth and shoot proliferation of Persian walnut, genotype Z60. Explant establishment was studied through a factorial experiment based on a completely randomized design with two factors including culture media (DKW and WPM) and BA concentration (0 (control), 0.5 and 1.0 µM). Single-node explants were prepared from the young branches of Z60 trees in the middle of May. Although the effect of two culture media on the growth characteristics had no significant difference, but mean comparison of the data with this regard showed that DKW medium performed better than WPM. No significant difference was observed between the two BA concentrations, but significant difference was between two concentrations (0.5 and 1.0 µM) with 0 (control). In shoot proliferation experiment, two cytokine hormones (BA and Kinetin) in three concentrations (4.4, 6.6 and 8.8 µM) were studied in DKW medium. The data obtained from this part of experiments were analyzed through unbalanced completely randomized design. The effect of different hormonal treatments and their concentrations on Z60 genotype growth characteristics showed that 8.8 µM BA was more effective in compared to the other two treatments. However, no significant difference was observed between the three concentrations with this respect.

Keywords

Genotype; Micro-propagation; Growth characteristic; Proliferation; Tissue culture