Cloning of luxAB Reporter Gene into Iranian Plant Pathogen Bacterial Isolates Pseudomonas syringae and Ralostonia solanacerum

پژوهش های حفاظت گیاهان ایران

Article 11, Volume 25, Issue 4 - Serial Number 17, December 2012   

Document Type: Research Article

DOI: 10.22067/jpp.v25i4.11864

Authors

S. Mostofi^* ; M. Mashreghi; M. Bahreini; F. Oroojalian; P. Pordeli

Abstract

Abstract
The ability to monitor particular microorganism in an environment is a difficult task. Many genes permit the differentiation of strains by conferring production of unique phenotypes such as bioluminescence [marine bacterial luciferase (luxAB)]. Therefore, lux-AB gene was cloned into two endemic plant pathogens Pseudomonas syringae and Ralostonia solanacearum by electrotransformation. lux-marking of above strains was carried out using miniTn-5 luxAB transposon. Purified strains were transformed with plasmid pUT containing luxAB gene by electroporator. Electroporation was performed in voltage 2/5 KV for 5 milisecend. All luxAB marked strains could grow on KB agar medium containing 12.5 µg/ml tetracycline and their luminescence intensity was measured by luminometer. lux-marked P. syringae and R. solanacearum were stable genetically engineered strains making them quite appropriate.

Keywords: Pseudomonas syringae, Ralostonia solanacerum, luxAB gene, strain monitoring