Experimental Determination of HT29 Cancerous Cell Surface Roughness by Atomic Force Microscopy to be Applied in Nanomanipulation

علوم کاربردی و محاسباتی در مکانیک

Article 8, Volume 28, Issue 1 - Serial Number 15, March 2017, Pages 111-122   

Document Type: مقاله کوتاه

DOI: 10.22067/fum-mech.v28i1.40826

Authors

Moharam Habibnejad Korayem¹; Hedieh Badkoobehhezaveh¹; Moein Taheri^{* 2}

¹Iran University of Science and Technology

²Arak University

Abstract

In this paper, surface roughness of HT29 cancerous is determined using topography images obtained by Atimic Force Microscopy (AFM). The mean radiuses of cell and substrate surface asperities are determined, based on results of conducted roughness analysis and Rabinovich roughness theory. These values have been applied in the equations of rough particle nanomanipulation dynamic model and the amounts of critical force and time are simulated. Based on results, the rolling mode is the dominant dynamic mode at the onset of cells movement and the analysis based on rabinovich model predict lower amounts of critical force and time compared to Rumpf model based analysis.

Keywords

Surface Roughness; HT29 cancerous cell; Atomic Force Microscopy; Nanomanipulation

References

1. D Antonio, P., Lasalvia, M., Perna, G., & Capozzi, V., "Scale-independent roughness value of cell membranes studied by means of AFM technique", Biochimica et Biophysica Acta (BBA)-Biomembranes, Vol. 1818(12), pp. 3141-3148, (2012).

2. Guduru, R. "In situ AFM Imaging of Nanoparticle-Cellular Membrane Interaction for a Drug Delivery Study", Master of Science thesis in biomedical engineering. Florida International University, (2011).

3. Mege, J. L., Capo, C., Benoliel, A. M., Foa, C., Galindo, R., & Bongrand, P., "Quantification of cell surface roughness; a method for studying cell mechanical and adhesive properties", Journal of theoretical biology,Vol. 119(2), pp. 147-160, (1986).

4. Kaul-Ghanekar, R., Singh, S., Mamgain, H., Jalota-Badhwar, A., Paknikar, K. M., & Chattopadhyay, S., "Tumor suppressor protein SMAR1 modulates the roughness of cell surface: combined AFM and SEM study", BMC Cancer, Vol. 9(1), pp. 1-12, (2009).

5. Korayem, M.H. & Zakeri, M., "Sensitivity analysis of nanoparticles pushing critical conditions in 2-D controlled nanomanipulation based on AFM", J Adv. Manuf. Technol, Vol. 41, pp. 714–726, (2009).

6. Korayem, M.H. & Zakeri, M., "Dynamic modeling of manipulation of micro/nanoparticles on rough surfaces”, J Applied Surface Science, Vol. 257(15), pp. 6503–6513, (2011).

7. Korayem, M.H., Badkoobeh Hezaveh, H. and Taheri, M., "Dynamic Modeling and Simulation of Rough Cylindrical Micro/Nanoparticle Manipulation with Atomic Force Microscopy", Microscopy and Microanalysis, Vol. 20 (6), pp. 1692-1707, (2014).

8. Sitti, M. & Hashimoto, H., "Force controlled pushing of nanoparticles: Modeling and experiments", In International Conference of Advanced Intelligent Mechatronics, pp. 13-20, (1999) Sep 19, Atlanta, GA.

9. Korayem, M.H., Motaghi, A. and Zakeri, M., "Dynamic modeling of submerged nanoparticle pushing based on atomic force microscopy in liquid medium", Journal of Nanoparticle Research, Vol. 13(10), 5009-5019, (2011).

10. Korayem, M.H., and Geramizadeh, M., "DNA Manipulation in Biological Liquid Environment Based on Atomic Force Microscopy", Nanoscience and Nanotechnology, Vol. 2(4), 109-115, (2012).

11. Beach, E.R., Tormoen, G.W., Drelich, J. and Han, R., "Pull-off Force Measurements between Rough Surfaces by Atomic Force Microscopy", Journal of Colloid and Interface Science 247, pp. 84–99, (2002).

12. Rabinovich, Y.I., Adler, J.J., Ata, A., Singh, R.K. & Moudgil, B.M. (2000a). Adhesion between nanoscale rough surfaces: I. Role of asperity geometry. J Colloid Interface Sci 232, 10–16, (2000).

13. Rabinovich, Y.I., Adler, J.J., Ata, A., Singh, R.K. & Moudgil, B.M. (2000b). Adhesion between nanoscale rough surfaces: II. Measurement and comparison with theory. J Colloid Interface Sci 232, 17–24, (2000).