Detection of Carnation etched ring virus in Greenhouses of Khorasan Razavi and Northern Khorasan Provinces by ELISA and PCR

پژوهش های حفاظت گیاهان ایران

Article 7, Volume 26, Issue 2 - Serial Number 16, June 2012   

Document Type: Research Article

DOI: 10.22067/jpp.v26i2.13818

Authors

M. Ashnayi^* ; B. Jafarpour; Saeed Malekzadeh Shafaroudi; A. Mirshamsi Kakhki; zeinab Heydariniya

Ferdowsi University of Mashhad

Abstract

In order to investigate the presence of Carnation etched ring virus (CERV), samples of carnation (Dianthus caryophyllus), Sweet William (Dianthus barbatus) and weed plants showing symptoms from greenhouses of Khorasan Razavi and Northern Khorasan provinces were collected in autumn 2009. Polyclonal antiserum was used in order to detection of CERV by Double- antibody sandwich-enzyme linked immunosorbent assay. Results showed that 54 samples (including carnation and Sweet william) out of 250 infected by this virus. In order to propagate the virus, we inoculated it on the indicator plants Saponaria vaccaria cv. Pink beauty, Silen armeria and Dianthus caryophyllus cv.Joker. Polymerase Chain Reaction (PCR) was carried out using specific primers for coat protein and movement protein genes of CERV. Two fragments of ~1500 bp and ~1000 bp for coat protein and movement protein were amplified by PCR, respectively.This is the first report of occurrence of CERV in greenhouses of Khorasan Razavi and Northern Khorasan provinces

Keywords

Carnation etched ring virus; Caulimovirus; PCR