بررسی الگوی بیان ژن‌های P5CS وCBF تحت تنش یخ‌زدگی و اثر خوسرمایی بر تغییرات میزان پرولین آزاد در گیاه نخود (Cicer arietinum L.)

پژوهش‌های حبوبات ایران

مقاله 2، دوره 8، شماره 2، بهار 1396، صفحه 31-43  

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/ijpr.v8i2.34624

نویسندگان

روح الله قاسمی ¹؛ عبدالرضا باقری²؛ نسرین مشتاقی²؛ فرهاد شکوهی فر³

¹دانشگاه تربیت مدرس

²دانشگاه فردوسی مشهد

³پژوهشکده علوم گیاهی دانشگاه فردوسی مشهد

چکیده

سازگاری گیاهان به تنش یخ‌زدگی از طریق تنظیم فعالیت ژن‌ها و متابولیسم سلولی انجام می‌شود. در این پژوهش، تغییرات بیان ژن‌های P5CS و (DREB1)CBF در شرایط دمایی معمولی (23‌درجه سانتی‌گراد)، خوسرمایی (10درجه سانتی‌گراد) و یخ‌زدگی (10-‌درجه سانتی‌گراد) در دو ژنوتیپ نخود زراعی مقاوم (MCC426) و حساس (MCC505) به سرما مورد ارزیابی قرار گرفت. نتایج آنالیز داده‌ها نشان داد که بیان این ژن‌ها در ژنوتیپ مقاوم در مقایسه با ژنوتیپ حساس به‌طور معنی‌داری بیشتر بود (P

کلیدواژه‌ها

بیان ژن؛ تنش یخ‌زدگی؛ پرولین؛ نخود زراعی

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