Investigation of Tomatinase Enzyme Using Thin Layer Chromatography(TLC) in Population of Fusarium oxysporum f.sp. lycopersici Race1 from Tomato in Northern Khorasan and Razavi Khorasan Provinces

پژوهش های حفاظت گیاهان ایران

Article 6, Volume 27, Issue 2 - Serial Number 20, June 2013, Pages 185-191   

Document Type: Research Article

DOI: 10.22067/jpp.v27i2.24539

Authors

N. Heidarzadeh^* ; H. Rouhani; M. Falahati Rastegar; E. Mahdikhani Moghaddam

Abstract

Abstract
This study was carried out of identify the tomatinase extracellular enzyme in Fusarium oxysporum f.sp. lycopersici Race1 the casual agent of wilting in tomato. 20 isolates of Fusarium oxysporum were recovered from infected roots,crown and stems of tomato plants collected from fields in major tomato producing areas in Northern and Razavi Khorasan province during 2009-2010 . Pathogenicity test done on cultivar Bonny Best and 17 isolates were pathogenic on tomato and 3 isolates were Nonpathogenic. These isolates tested on cultivars Bonny Best,Datura, Cicer and Nightshade, proved to be Fusarium oxysporum f.sp. lycopersici. Patogenic test on differential hosts done and confirmed the existence of race1 in Khorasan province. For TLC analysis these isolates loaded on silicagel paper with 2 standards including: α- tomatine and tomatidine that there all had tomatinase activity and 2 spots of these isolates seen on plate.

Keywords

Fusarium oxysporum، Tomatinase; Tomato; Chromatography