Scrutiny and Amplification of the Myostatin gene pro-motor region and sequential analysis in Khuzestan province local Cattles

پژوهشهای علوم دامی ایران

Article 1, Volume 6, Issue 3 - Serial Number 19, September 2014, Pages 265-269   

Document Type: Genetics & breeding

DOI: 10.22067/ijasr.v6i3.43297

Authors

Zeinab Mohammadi^* ; Hedayatollah roshanfekr¹; Mohammad Taghi Beigi Nasiri²; Hamid Rajabi Meamari

¹Department of Animal Science, University of Khuzestan Agricultural Sciences and Natural Resources, Khuzestan, Iran.

²Department of Animal Sciences, Ramin Agriculture and Natural Resources University, Khuzestan, Khuzestan, Iran.

Abstract

The aim of the current study is to amplification the regularity region encompassed the mutations changing the Myostatin gene express and also to determine sequential analysis. The blood samples taken from 12 Najdi Cattles in Najdi Cattle station in Shushtar city, Khuzestan province. Then the DNA extracted to amplification 730bp and 561bp fragments. Sequencing was performed after the precision in PCR products put-upon Agarose gel 1% and Myostatin gene pro-motor was determined. In Myostatin gene’s pro-motor region, three mutations, Insertion, Deletion and Substitution in 561bp fragment and the two mutations, Deletion and Substitution in 730bp were appeared, results pointed out. Reported mutations on boxes and pro-motor components did not influence upon Myostatin gene.

Keywords

Myostatin gene; Mutation; Doubled-muscle; Najdi Cattle

References

- صوفی، ب.، م. محمدآبادی، ک. شجاعیان، ا. باقی زاده، س. فراستی، ن. عسگری، و ا. دیانی. 1388. ارزیابی چند شکلی ژن میوستاتین در گوسفند نژاد سنجابی با استفاده از روش PCR- RFLP. مجله پژوهش های علوم دامی.جلد19. ش1. ص: 88-79.

2- Arthur, P.F., M. Makarechian, and M.A. Price. 1988. Incidence of dystocia and prenatal calf mortality resulting from reciprocal crossing of double-muscled and normal cattle. Canadian Veterinary Journal. 29: 163-167.

3- Crisa, A., C. Marchitelli, M.C. Savarese, and A.Valentini. 2003. Sequenseanalaysis of myostatin promoter in cattle. Cytogenet Genome Res. 102: 48-52.

4- Grobet, L., D. Poncelet, J.L. Royo, B. Brouwers, D. Pirottin, C. Michaux, F. Menissier, M. Zanotti, S. Dunner, and M. Georges. 1998. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double muscling in cattle. Mamm Genome. 9:210–213.

5- Joulia-Ekaza, D, and G. Cabello. 2006. Myostatin regulation of muscle development: Molecular basis, Natural mutations, Physiopathologicalacpects. Exp Cell Res. 312: 2401-241410.

6- Mcpherron, A. C., A.M. Lawler, and S.J. Lee. 1997. New regulation of skeletal muscle mass in mice by aTGF-beta superfamily member. Nat. 387: 83-90.

7- Masumi, N., Y. Akihiro, N. Shinichiroum, Y. Tsutomu, N. Takashi, I. Mitsuo, M. Keiji, O. Hideyo, and N. Sumihare. 2002. A missense mutant myostatin causes hyperplasia without hypertrophy in the mouse muscle. BiochemBioph Res Co. 293: 247-251.

8-Pamela, W., A. S. Judith, M.. L. Alyson, W. J. Arthur, and W. J. Lewis. 2001. The role of the myostatin gene in the South Devon Breed. Genet. Sel. Evol. 34: 221-232.

9-Rong, D., D. Juan, Q. Jian, C. Li-Chun, H. Jian, J. Hong,,and A. Xiao-Rong. 2011. Molecular cloning and sequence analysis of the cat myostatin gene 5′ regulatory region. Afr J Biotechnol vol. pp: 10366-10372.

10- Stefania, D., F. Luca, N.C. Leonardo, T. Marco, M. Laura, and F. Adalberto. 2010. Analysis of Horse myostatin gene and identification of single nucleotide polymorphisms in breeds of different morphological types. Journal Biomed BiotechnolVol 2010. Article ID 542945, p: 11-15.

11- Smith, J.A., A. M. Lewis, P. Wiener, and J. L. Williams. 2000. Genetic variation in the bovine myostatin gene in UK beef cattle. allele frequencies and haplotype analysis in the South Devon. Anim Genet. 31:306–309.