The effect of biological and chemical nitrogen fertilizer on yield and yield components of improved chickpea varieties under rainfed conditions

پژوهش‌های حبوبات ایران

Article 4, Volume 10, Issue 2, December 2020, Pages 49-61   

Document Type: Original Articles

DOI: 10.22067/ijpr.v10i2.59163

Authors

Sayyed Hossain Sabaghpour^{* 1}; Abolfazl Sherifi²; Davood Aradatmand Asli³

¹Agricultural and Natural Resources Research and Education of Center of Hamedan Province, Hamedan, Iran

²Department of Agronomy and Plant Breeding, Islamic Azad University of Saveh, Iran

³Department of Agronomy, Payame Noor University, Tehran, Iran

Abstract

Inroduction
Excessive use of chemical fertilizers for increasing agricultural production causing environmental damage and disturbance in the soil fertility. Currently, biological approaches for improving crop production are gaining strong status among agronomists and environmentalists following integrated plant nutrient management system. Chickpea is the most important pulse crop which is grown on 463,000 hectares in Iran. Major chickpea areas (98%) are planted under rainfed condition and are grown in rotation with cereals mainly wheat and barley. Mean productivity of chickpea 402 kg/ha in 2014-15 cropping season in Iran. One of the reason for low chickpea productivity in Iran, is unsuitable using nutrition in chickpea farms. Currently biologic fertilizers as an alternative option for chemical fertilizer to enhance to soil fertility in the stable agricultural production are considered. Rhizosphere beneficial bacteria are commonly called plant growth promoting rhizobacteria (PGPR) and have been under researchers focus for many years. PGPRs can stimulate plant growth through different mechanisms. Solubilization of inorganic phosphate is a characteristic has been frequently used for screening these bacteria. The objective of present study was to investigate the effect of biological and chemical nitrogen fertilizers on yield and yield components of improved chickpea varieties.
 
Materials & Methods
An experiment was carried out in factorial based on a randomized complete block design with four replications and five fertilizers treatments (control, Super Nitro Plus, Nitroxin, Rhizocheck and 30 kg nutrition fertilizer per hectare) and three chickpea varieties (Azad, Hashem and Arman) in Ekbatan Research Station of Hamedan under rain-fed condition during 2012-2013. Rhizocheck had 10⁷ bacteries from Pseudomonas putida and 10⁸ Mesorhizobium cicero and Super Nitro Plus included 10⁸ Bacillus subtilis, pseudomonas fluorescens and different species of Azospirillum. Also Nitroxin covered 10⁸ nitrogen fixing bacteries such as Azotobacter and Azospirillum. During vegetative and reproductive phases, weeds were controlled and data were recorded on days to flowering, days to maturity, plant height, harvest index, number pods per plant, number of seeds per pod, 100-seed weight, and seed yield. Data were analyzed using the SAS. Ver. 9.1 and figures were drawn by EXCEL and means compared by using DMRT at the 5% probability level.
 
Results & Discussion
The results showed that fertilizer had significant effects on harvest index and seed yield at 1% level of probability. But fertilizer treatment had not significant effects on days to flowering, days to maturity, leaf area index, plant height, pods number per plant, seeds number per pod and 100-seed weight. Also the results showed that varieties showed significant difference for days to flowering, leaf area index, harvest index, seeds  number per pod, and seed yield. Varieties had not significance different on days to maturity, plant height, number of pod per plant and 100-seed weight. Interaction of fertilizer × variety on all characters was not significant. The result of mean comparison showed that the highest seed yield has been produced by Rhizochick consumption. The utilization of Rhizocheck had result in 39 and 26 percent higher harvest index and seed yield than control, respectively. Azad variety had the highest leaf area index, harvest index and seed yield. The lowest leaf area index, harvest index and seed yield was observed on Hashem variety. Stephan (2000) reported that 36 percent seed yield increased due to rhizobium inoculation on chickpea seed. Maximum seed yield was achieved in Azad variety with Mesorhizobium of SWRI-15 which produced 151 percent higher than check. Rhizobium inoculation in comparison with no inocultion on chickpea seed under drought stress condition increased seed yield. Most of scientists reported that using biological fertilizers have been effected on seed yield in comparison with check on lentil, sunflower, wheat, corn and pearl millet.
 
 Conclusion
One of the most important factors for soil fertility is microorganism ability for production and plant growth promoting. The result of present study showed the highest productivity obtained from Rhizochick consumption. Azad improved chickpea variety produced the highest seed yield. Rhizochick may cause photosynthesis intensive activity and enhancing availability elements. Therefore seed yield have been increased. Generally, PGPR promote plant growth directly by either facilitating resource acquisition (nitrogen, phosphorus, essential element) or modulating plant hormone level. The reduction chemical consumption will affect decreasing environment contamination which is one of the important factors for stability agriculture. Therefore Azad improved chickpea variety with Rhizochick inoculation in Hamaden province is recommended.

Keywords

Chickpea; Cicer arietinum; Nitroxin; Rhizochick; Super Nitro Plus

References

1. Asgharzadeh, A., and SalehRastin, N. 2000. Investigation of potential of symbiosis nitrogen fixation of indigenous Mesorhizobium ciceri with two varieties of Cicer arietinum in Iran. Soil and Water Journal 12(43): 7-33. (In Persian).
2. Bairley, L.D. 1988. Influence of single strains a commercial mixture of Bradyrhizobium japonicum on growth, nitrogen accumulation and nodulation of two early maturing soybean cultivars. Candian Journal Plant Science 68: 411-418.
3. Bremer, E., and Kessel, C.V. 1990. Selection of Rhizobium leguminosarum strains for lentil under growth room and field conditions. Plant and Soil 121: 47-56.
4. Chen, J. 2006. The combined use of chemical and organic fertilizers and/or biofertilizer for crop growth and soil fertility. International Workshop on Sustained Management of the Soil-Rhizosphere System for Efficient Crop Production and Fertilizer U 16-30. Thailand, 11 pp.
5. Diaz, M., Virginia, M., and Canigia, F. 2009. Field performance of a liquid formulation of Azospirillum brasilensilense on dryland wheat productivity. European Journal of Soil Biology 45 (1): 3-11.
6. Elhadi, E.A., and Elsheikh, E.A.E. 1999. Effect of Rhizobium inoculation nitrogen fertilizater on yield and protein content of six chickpea (Cicer arietinum) cultivars in marginal soils under irrigation. Nutrient Cycling in Agroecosystems 54: 57-63.
7. Elsheikh, E.A.E., and Hadi, E.A. 1999. Effect of Rhizobium inoculation and nitrogen fertilization on yield and protein content of six chickpea (Cicer arietinum) cultivars in marginal soils under irrigation. Nutrient Cycling in Agroecosystems 54: 57-63.
8. Faraji, A., and Arzanesh, M.H. 2013. Response of two canola genotypes to plant growth promoter bacteria (Azospirillum spp): seed yield and its components, dry matter and harvest index. Seed and Plant Production Journal 29(1): 17-29. URL: http:// sppi.sp[[.ir/article-1-324-fa.html. (In Persian).
9. Ghasemzadeh-Ganjehie, M. and Asgharzadeh, A. 2013. Effects of rhizobiums sush inoculation and fertilizer on yield and yield components of chickpea (Cicer arietinum) in Khorasan-Razavi. Irainain Journal of Pulses Research 4(1): 51-58. (In Persian).
10. Hamidi, A., Ghalavand, A., Dehghan Shoar, M., Malakoti, M.J., Asgharzadeh, A., and Chogan, R. 2006. The effects of application of plant growth promoting rhizobacteria (PGPR) on the yield of fodder maize (Zea mays L). Pajouhesh and Sazandgi 4: 16-22. (In Persian).
11. Hamzei, J., Najjari, S., Sadeghi, F., and Seyedi, M. 2014. Effect of foliar application of nano-iron chelate and inoculation with mesorhizobium bacteria on root nodulation, growth and yield of chickpea under rainfed conditions. Iranian Journal of Pulses Research 5(2): 9-18. (In Persian).
12. Karimi, M., and Siddique, K. 1991. Crop growth and relative growth rates of old and modern wheat cultivars. Australian Journal of Agriculture Research 42: 13-20.
13. Khandan Bejandi, T., Seyed Sharifi, R., Sedghi, M., Asgari Zakaria, R., Namvar, A., and Jafari Moghaddam, M. 2010. Effect of plant density, rhizobia and microelements on yield and some of morph physiological characteristics of chickpea. Electronic Journal of Crop Production 3(1): 139-157.
14. Khodarahmi, M., Sabaghpour, S.H.,and Farnia, A. 2013. Effect of different strains of Rhizobium on seed yield and its components of improved chickpea (Cicer arietinum) Cultivars. Seed and Plant Journal Production 29(3): 403-412. ‌(In Persian).
15. Koutroubas, S.D., Parageorgiou, M., and Fotiadis, S. 2009. Growth and nitrogen dynamics of spring chickpea genotypes in a Mediterranean-type climate. Journal of Agriculture Science 147: 445-458.
16. Mehrpouyan, M., Noormohammadi, Gh., Mirhadi, M.J., Heidari Sharifabad, H., and Shirani Rad, A.H. 2011. Effect of some inoculants containing Rhizobium legominosarum; bv. Phaseoli on nutrients elements uptake in three cultivars of common bean. Irainain Journal of Pulses Research 1(2): 1-10. (In Persian).
17. Ministry of Jihad-e-Agriculture. 2016. Agricultural Statistics for Agronomy, 2014-15. Department of Statistics and Information. 163 pp. (In Persian).
18. Mirshekari, B., Baser, S., and Javanshir, A. 2009. Effect of seed inoculation with nitragin and different levels of urea on physiological traits and biologic yield of maize, cv. 704 grown in cold and semi-arid regions. New Finding in Agriculture 3(4): 403-411. (In Persian).
19. Nezarat, S., and Gholami, A. 2008. Evaluation of Azospirillum and Pseudomonas on maize growth. 2^nd National Congress of Ecological Agriculture in Iran, pp. 2037-2049. (In Persian).
20. Oliveira, C.A., Alves, M.C., Marriel, I.E., Gomes, E.A., Scotti, M.R., Carneiro, N.P., Guimaraes, C.T., Schaffert, R.E., and Sa, N.M. 2008. Phosphate solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome. Soil Biology and Biochemistry 3 (1): 6-24.
21. Pourakbar, L., Khayami, M., and Jelil, Kh. 1999. The interaction of Cu and EDTA on K+ leakageand some metals content in root and shoot of Maize (Zea mays) seedlings. Journal of Sacience of Tarbiet Molalm 8(2): 121-132. (In Persian).
22. Rai, S.N., and Gaur, A.C. 1999. Characterization of Azotobacter and effect of Azotobacter and Azospirilium as inoculant on the yield and N-up tke of wheat crop. Plant and Soil 109: 131-134.
23. Rajendran, K., and Devaraj, P. 2004. Biomass and nutrient distribution and their retur of Casuarina equisetifolia inoculated with biofertilizer in farm land. Biomass and Bienergy 26: 235-249.
24. Romdhane, S.B., Trabelsib, M., Elarbi, M., Lajudie, P., and Mhamdia, R. 2009. The diversity of rhizobia nodulating chickpea (Cicer arietinum) under water deficiency as a source of more efficient inoculants. Soil Biology & Biochemistry 41: 2568-2572.
25. Rosety, D., Gaurand, R., and Juhri, N. 2006. Plant growth stage, fertilizer management and bio-inoculation of arbascular mycorrizal fungi and plant growth promoting rhizobacteria affect rhizobacteria community structure in rain-fed wheat field. Soil Biology & Biochemistry 38: 1111-1120.
26. Rupela, O.P., and Saxena, M.C. 1987. Nodulation and Nitrogen Fixation in C In: M.C. Saxena and K.B. Singh (Eds.). The Chickpea. 191-206 pp., CAB International, Wallingford, Oxon.
27. S.H. 1997. Genetic of Chickpea. Nasher, 54 pp. (In Persian).
28. Sabaghpour, S.H., Malhotra, R.S., and Banayi, T. Registration of Hashem Kabuli chickpea. Crop Science 45: 6.
29. S.H., Malhotra, R.S., Sarparast, R., Safikhani, M., Alizadeh, S.H., Jahangeri, A. and Khalaf, G. 2006. Registration of ‘Arman’- A Kabuli chickpea cultivar. Crop Science 46: 2704-2705.
30. Sabaghpour S.H., Safikhani, M., Pezeshkpour, P., Jahangiri, A., Sarparast, R., and Karami, I. 2010. Azad, A new chickpea cultivar for dryland moderate and semi warm climate of Iran. Seed and Plant Improvement Journal 26 (2): 293-296. URL: http://spij.spii.ir/article-1-456-fa.html. (In Persian).
31. Sabaghpour, S.H. 2015. Strategic Framework for Food Legume R Chap & Entesharat Organization, 412 pp. (In Persian).
32. Saberi, H., Mosenabadi, Gh., Majidian, M., and Ehteshami, S.M. 2015. Integrated application of biological and chemical fertilizers on bean (Phaseolus vulgaris) under Rasht climate conditions. Iranian Journal of Pulses Research 6(1): 21-31. (In Persian).
33. Sajadi Nik., R., Yadavi, A., Balouchim, H.R., and Farajee, H. 2011. Effect of chemical (urea), organic (vermicompost) and biological (nitroxin) fertilizers on quantity and quality yield of sesame (Sesamum indicum). Sustainable Agriculture and Production Science 21(2): 87-101. (In Persian).
34. Saxena, P.K., and King, J. 1987. Morphogenesis in Lentil plant regeneration from callus on Lens culinaris Medik via somatic embryogenesis. Plant Sciences 52: 223-227.
35. Shahbazi, H.O. 2015. The effect of biological and chemical nitrogen fertilizers on yield and yield components of lentil genotypes. MSc. Thesis, Faculty of Agriculture, Department of Agronomy and Breeding. Azad University, Savah Branch, Iran, 128 pp. (In Persian).
36. Shakeri, E., Amini Dehaghi, M., and Modares Sanavi, S. 2012. Effect of chemical fertilizer and biofertilizer on seed yield, its components, oil and protein percent in sesame varietes. Sustainable Agriculture and Production Science 22(1): 71-85. (In Persian).
37. Sharman, A.K. 2004. Biofertilizers for Sustainable A Agrobios. India.
38. Solemani, R., and Asgharzadeh, A. 2010. Effects of Mesorrhizobium inoculation and fertilizer application on yield and yield components of rainfed chickpea. Iranian Journal of Pulses Research 1(1): 1-8. (In Persian).
39. Stephan, K.B. 2000. Evaluation of Granular Rhizobium Inoculant for C PhD. Thesis, University of Saskatchewan, Canada.
40. Subba Rao, N.S. 1988. Biofertilizers in A Oxford and IBH Publishing Co. New Delhi India.
41. Suliman, R., and Bajwa, R. 2010. Appraisal of two Pseudomonas species as a biofertilizer for sunflower (Helianthus annuus). International Journal of Biology and Biotechnol 7(1,2): 49-52.
42. Yasari, E., and Patwardhan, A.M. 2007. Effects of Aztobacter and Azospirillium inoculations and chemical fertilizers on growth and productivity of Canola. Asia Journal of Plant Science 6(1): 77-82.
43. Yousry, M., Kabesh, O.M., and Saber, M.S. 1978. Manganese availability in a calcareous soil as a result of phosphate fertilization and inoculation with Phosphobacterin. African Journal of Agricultural Science 5(2): 1386-1392.
44. Zahir, A.Z., Arshad, M., and Frankenberger, W.F. 2004. Plant growth promoting rhizobacteria: applications and perspectives in agriculture. Advances in Agronomy 81: 97-168.