The effect of inoculation with Growth Promoting Bacteria, Mycorrhiza and Phosphorus on yield and yield components of Mungbean (Vigna radiata L.)

پژوهش‌های حبوبات ایران

Article 11, Volume 11, Issue 1 - Serial Number 21, June 2020, Pages 134-151   

Document Type: Original Articles

DOI: 10.22067/ijpr.v11i1.73888

Authors

Fahieme Rezapoorian^* ; Seroulah Galeshi; Ebrahim Zeinali; Beniamin Torabi

Department of Agronomy, Faculty of Crop Production, Gorgan University of Agricultural Sciences and Natural Resources, Iran

Abstract

Introduction
Mungbean (Vigna radiata L.) is an important legume crop. It is a great source of proteins, vitamins and minerals. The seed contains 24% protein content, 1.30% fat and 60.4% carbohydrates; calcium is 118 and phosphorus is 340 mg per 100 g of seed. Its capacity to restore soil fertility through nitrogen fixation makes it a valuable crop. Phosphorus is considered to be the most limiting element after nitrogen for the plant. However, a large amount of phosphorus in chemical fertilizers occurs after entering the insoluble soil. Converting soil insoluble phosphates to a form available for plants is a necessary goal to achieve sustainable agricultural production. Biofertilizers, are considered as a new technology to increasing nutrients in the soil and reduce the use of chemical fertilizers. Biofertilizers keep the soil environment rich in all kinds of micro and macro nutrients, via nitrogen fixation, phosphate and potassium solubilisation or mineralization, release of plant growth regulating substances, production of antibiotics and biodegradation of organic matter in the soil. The main objective of this study was to evaluate biofertilizers inoculation.
 
Materials & Methods
To study the effect of Plant Growth Promoting Bacteria, Mycorrhizal fungi and different levels of fertilizer phosphorus on the yield and yield components mung bean (Vigna radiata L.) a field experiment was carried out as a factorial based on a randomized complete block design (RCBD) with three replications at research field of the Faculty of Agriculture of Gorgoan University in 2016 and 2017. The treatments of this research consisted of three levels of phosphorus (Control, 150 Kg.h-1 and 225 Kg.h-1) and eight levels of Growth Promoting Bacteria and Mycorrhizal fungi (Control, Azospirillum lipoferum, Pseudomonas fluoresens, Glomus mosseae, Azospirillum and Pseudomonas, Azospirillum and Glomus mosseae, Pseudomonas and Glomus mosseae, and Azospirillum lipoferum, Pseudomonas fluoresens, Glomus mosseae). In this experiment, Rhizobium bacteria (R. leguminosarum) was used in all plots. Grain inoculation was done in shadow and after drying, inoculated grains were immediately cultivated. Mycorrhizal fungi was applied under the grain hole just prior to sowing. Chemical fertilizers were applied at a rate of 50 and 100 Kg.h-1 in N and K respectively. At harvest, random samples of 10 plants for each experimental unit were taken and plant height, pod length, number of pods per plant, number of seed per pod, 1000- seed weight, biological yield, seed yield and harvest index were recorded. Data were subjected to analysis of variance procedure using the SAS statistical software and for comparing the mean effects of interactions with Slicing method, in order to compare the mean of simple effects, the least significant difference test method (LSD) was used at 5% probability level.
 
Results & Discussion
The results of experiment revealed that the effect of year, phosphorus fertilizer and biological fertilizer showed a significant effect on yield and yield components, plant height, pod length and protein content. The results also showed that the interaction of biofertilizer and phosphorus on plant height, biological yield and harvest index were significant. The results of the comparison of the mean of data in the first year showed that the highest grain yield (226 g.m-2) was obtained in inoculum treatment with Azospirilum and Mycorrhiza fungi at a consumption level of 225 Kg.h-1 phosphorus fertilizer, Which increased by 29.6% compared to the control. In the second year of experiment, the highest grain yield (216 g.m-2) was obtained with 38.7% increase in comparison with control in inoculation with Azospirilum, Pseudomonas and Mycorrhiza fungi at 225 Kg.h-1 phosphorus fertilizer level. The results of the comparison of the mean of the data in the first year showed that the highest number of pods per plant, grain yield, biological yield, harvest index and seed protein were related to Azospirillum and Glomus mosseae inoculation, and in the second year they were treated with Azospirillum lipoferum, Pseudomonas fluoresens, Glomus mosseae. Also The results of the comparison of the mean of the data showed that the highest 1000-seed weight (52.9 g), number of seeds per pod (8.5 Number in pods) in 225 Kg.h-1 treatment of phosphorus fertilizer.
Conclusion
Biofertilizer are beneficial bacteria and fungus that colonize plant roots and enhance plant growth by a wide variety of mechanisms. The use of biofertilizer is steadily increasing in agriculture and offers a new way to replace chemical fertilizers, pesticides, and supplements. According to these results, it can be concluded that the best treatment for mung bean cultivation and reducing the use of phosphorus fertilizer is the use of Azospirilum and Mycorrhizal fungus. Application Azospirilum and Mycorrhizal fungus in this study caused to increase the seed yield and protein content of seed.

Keywords

Azospirillum lipoferum; Glomus mosseae; Legume; Pseudomonas fluoresens

References

1. Abbasi, N.A., Jalilian, J., and Zare, M.J. 2016. Evaluation of bacterial efficacy in separate inoculation and in combination with Bacillus megaterium phosphate solubilizing bacteria on reducing the effect of salinity stress in mungbean. (Vigna radiata L.). Journal of Environmental Plant Physiology 41: 26-40. (In Persian).

2. Aboutalebian, M.A., and Elahi, M. 2015. Replacement of phosphate fertilizer application by bio-fertilizers in chickpea production under on-farm seed priming conditions. Iranian Journal of Filed Crop Science 46(3): 381-394. (In Persian with English Summary).

3. Alami-Milani, M., Amini, R., and Bandehagh, A. 2014. Effect of bio-fertilizers and combination with chemical fertilizers on grain yield and yield components of pinto bean (Phaseolus vulgaris L.). Special Issue of Agricultural Science and Sustainable Production p. 15-29. (In Persian with English Summary).

4. Amanullah, A., Aziz, K., Saifullah, K., Munir A., and Jahangir, K. 2012. Biofertilizer a possible substitute of fertilizers in production of wheat varietyzaardan in balochiistan substitute in balochistan. Pakistan Journal of Agricultural Research 25(1): 44-49.

5. Baloch, A.F., Larik, K.A., and Jamro, G.H. 2006. Response of three mustard (Brassica Juncea L.) varieties to N and P fertilizer levels. Pakistan Journal of Agriculture 22: 1058-1062.

6. Belachew, T., and Abera, Y. 2011. Effect of green manuring in combination with nitrogen on soil fertility and yield of bread wheat (Triticum aestivum) under double cropping system of Sinanadinsho, Southeast Ethiopia. Journal of Biodiversity and Environmental Sciences 1(1): 1-11.

7. Bhattarai, N., Baral, B., Shrestha, G., and Yami, K.D. 2011. Effect of mycorrhiza and rhizobium on Phaseolus vulgaris L. Scientific World 9( 9).

8. Bona, E., Lingua, G., Manassero, P., Cantamessa, S., Marsano, F., Todeschini, V., Copetta, A.D., Agostino, G., Massa, N., Avidano, L., Gamalero, E., and Berta, G. 2015. AM fungi and PGP Pseudomonads increase flowering, fruit production, and vitamin content in strawberry grown at low nitrogen and phosphorus levels. Mycorrhiza 25: 181-193.

9. Carvalho, M.C.S., Nascente1, A.S., Ferreira, G.B., Mutadiua, C.A.P., and Denardin, J.E. 2018. Phosphorus and potassium fertilization increase common bean grain yield in Mozambique. Revista Brasileira de Engenharia Agricola e Ambiental 22(5): 308-314.

10. Creus, C., Pereyra, M., Casanovas, E., Sueldo, R., and Barassi, C. 2010. Plant Growth-Promoting effect of Rhizobacteria on abiotic stressed plants. Azospirillum-grasses model. The Americas Journal of Plant Science and Biotechnology 4(1): 49-59.

11. Elias, F., Muleta, D., and Woyessa, D. 2016. Effects of phosphate solubilizing fungi on growth and yield of Haricot bean (Phaseolus vulgaris L.) plants. Journal of Agricultural Science 8(10): 204-218.

12. Fanay, H.R., Pieri, A., and Narouy Rad, M.R. 2013. The effect of different amounts of phosphorus fertilizer on grain yield and some agronomic characteristics of oil Hindi mustard (Brassica juncea L.) under drought stress. Journal of Environmental Stresses in Crop Sciences 6(2): 147-157. (In Persian).

13. Farzana, Y., and Radizah, O. 2005. Influence of Rhizo bacterial inoculation on growth of the sweet potato cultivar. Journal of Biological Sciences 1(3): 176-179.

14. Gangwar, R.K., Bhushan, G., Singh, J., Upadhyay, S.K., and Singh, A.P. 2013 Combined effects of plant growth promoting rhizobacteria and fungi on mung bean (Vigna radiata L.). International Journal of Pharmaceutical Sciences and Research 4: 4422-4426.

15. Ghasemi, S., Siavoshi, K., Choukan, R., and Khavazi, K. 2011. Effect of biofertilizer phosphate on grain yield and its components of maize (Zea mays L.) cv. KSC704 under water deficit stress conditions. Seed Plant Prod Journal 27-2(2): 219-233.

16. Gupta, G., Parihar, S. S., Ahirwar, N.K., Snehi, S.K., and Singh, V. 2015. Plant Growth Promoting Rhizobacteria (PGPR): current and future prospects for development of sustainable agriculture. Microbial & Biochemical Technology 7: 096-102.

17. Habibzadeh, Y., Mamaghani, A.R., and Kashani, A. 2007. Effects of different densities on grain yield and yield components and protein in three cultivars of Mung bean (Vigna radianta L.) in Ahvaz region. Agricultural Scientific Journal 30(3): 1-13.(In Persian).

18. Kamboj, N., and Malik, R.S. 2018. Influence of phosphorus and boron application on yield, quality, nutrientn content and their uptake by Green Gram (Vigna radiate L.). International Journal of Current Microbiology and Applied Sciences 7(3): 1451-1458.

19. Kanwal, S., Bano, A., and Malik, R. 2015. Effects of arbuscular mycorrhizal fungi on wheat growth, physiology, nutrition and cadmium uptake under increasing cadmium stress. International Journal of Agronomy and Agricultural Research 7(5): 30-42.

20. Karimi, K., Bolandnazar, S., and Ashoori, S. 2013. Effect of bio-fertilizer and arbuscular mycorrhizal fungi on yield, growth characteristics and quality of Green Bean (Phaseolous vulgaris L.). Journal of Agricultural Science and Sustainable Production 23(3): 157-167. (In Persian with English Summary).

21. Kaur, N., Sharma, P., and Sharma, S. 2015. Co-inoculation of Mesorhizobium sp. and plant growth promoting rhizobacteria Pseudomonas sp. as bio-enhancer and bio-fertilizer in chickpea (Cicer arietinum L.). Legume Research 38: 367-74.

22. Khajeh, M., Gholipour, S., Amiri, A., Yadollahi Dehcheshmeh, P., Mighani, H., and BarajeFard, M. 2016. The impact of biological and chemical phosphorus fertilizers on quality of mung been Gohar variety. Journal of Agricultural Research 8(1): 1-14. (In Persian with English Summary).

23. Moustaine, M., Elkahkahi, R., Benbouazza, A., Benkirane, R., and Achbani, E.H. 2017. Effect of Plant Growth Promoting Rhizobacterial (PGPR) inoculation on growth in tomato (Solanum lycopersicum L.) and characterization for direct PGP abilities in Morocco. International Journal of Environment, Agriculture and Biotechnology 2(2): 590-596.

24. Mrkovacki, N., and Milic, V. 2001. Use of Azotobacter chroococcum as potential useful in agricultural application. Annals of Microbiology 51: 145-158.

25. Naidu, N., Grosoiah, V., Satyanarayna, A., and Raja Rajeswari, V. 1993. Variation in developmental and morpho-physiological traits under different environments and their relation to grain yield of green gram (Vigna radiata L.). Indian Journal of Agricultural Science 63(8): 473-478.

26. Nasrollahzadeh Asl, A. 2017. Effects of nitrogen and phosphate biofertilizers on morphological and agronomic characteristicsof Sesame (Sesamum indicum L.). Open Journal of Ecology 7: 101-111.

27. Noonari, S.H., Kalhoro, S.H., Mahar, A.A., Raza, S., Ahmed, M., Shah, S.F.A., and Baloch, S.U. 2016. Effect of different levels of phosphorus and method of application on the growth and yield of wheat. Natural Science 8: 305-314.

28. Parsa Motlagh, B., Mahmoudi, S., Sayari Zahan, M.H., and Taghizadeh, M. 2011. Effect of mycorrhiza and P fertilizer on the concentration of photosynthetic pigments and nutrients of beans (Phaseolus vulgaris L.) under salinity stress conditions. Journal of Agroecology 3(2): 233-244. (In Persian).

29. Rahimi, M.M., and Hashemi, A.R. 2015. Yield and yield components of Vetch (Vigna radiata) as affected by the use of vermicompost and phosphate bio-fertilizer. Journal of Crop Ecophysiology 10(2): 529-540. (In Persian with English Summary).

30. Rajabzadeh Motlagh, F. 2011. Evaluation application of arbuscular mycorrhiza, nitrogen fixing bacteria and nitrogen fertilizer on yield and yield component of Phaseolus vulgaris. MSc. Thesis. Faculty of Agriculture, Shahrood University of Technology, Iran.

31. Rostamikia, Y., Tabari Kouchaksaraei, M., Asgharzadeh, A., and Rahmani, A. 2016. The effect of Plant Growth-Promoting Rhizobacteria on growth and physiological characteristics of Corylus avellana seedlings. Ecopersia 4(3): 1471-1479.

32. Saxena, J., Saini, A., Kushwaha, K., and Arino, A. 2016. Synergistic effect of plant growth promoting bacterium Psedomonas flurescens and phosphate solubilizing fungus Aspergillus awamori for growth enhancement of chickpea. India Journal of Biocheistry and Biophysics 53: 135-143.

33. Seema, K., Mehta, K., and Singh, N. 2018. Studies on the effect of Plant Growth Promoting Rhizobacteria (PGPR) on growth, physiological parameters, yield and fruit quality of strawberry cv. Chandler. Journal of Pharmacognosy and Phytochemistry 7(2): 383-387.

34. Singh, R., Singh, P., Singh, V., and Yadav, R.A. 2018. Effect of phosphorus and PSB on yield attributes, quality and economics of summer greengram (Vigna radiata L.). Journal of Pharmacognosy and Phytochemistry 7(2): 404-408.

35. Singh, Z. 2016. Growth and yield of Desi chickpea (Cicer arietinum L.) in relation to phosphorus and plant growth promoting rhizobacteria. MSc. Thesis in Agronomy. 81 p.

36. SoleimaniFard, A., Naseri Rad, H., Naseri, R., and Pieri, A .2013. Effect of growth promoting bacteria on phenology, yield and yield components of corn hybrids (Zea mays L.). Journal of Crop Ecophysiology 7(1): 71-90. (In Persian).

37. Srivastava, A.K., Singh, T., Jana, T.K., and Arora, D.K. 2011. Induced resistance and control of charcoal rot in chickpea (Cicer arietinum) by Pseudomonas fluorescence. Canadian Journal of Botany 7: 787-795.

38. Stefan, M., Munteanu, N., Stoleru, V., and Mihasan, M. 2013. Effects of inoculation with Plant Growth Promoting Rhizobacteria on photosynthesis, antioxidant status and yield of runner bean. Romanian Biotechnological 18(2).

39. Suhag, M. 2016. Potential of biofertilizers to replace chemical fertilizers. International Advanced Research Journal in Science, Engineering and Technology 3(5): 163-167.

40. Suryantini. 2016. Effect of phosphorus, organic and biological fertilizer on yield of Mungbean (Vigna Radiata L.) under two cropping patterns. Nusantara Bioscience 8(2): 273-27.

41. Tohidinia, M.A., Mazaheri, D., Bagher-Hosseini, S.M., and Madani, H. 2014. Effect of biofertilizer Barvar-2 and chemical phosphorus fertilizer application on kernel yield and yield components of maize (Zea mays cv. SC704). Iranian Journal of Crop Sciences 15(4): 295-307. (In Persian with English Summary).

42. Wang, X., Pan, Q., Chen, F., Yan, X., and Liao, H. 2011. Effects of co-inoculation with arbuscular mycorrhizal fungi and rhizobia on soybean growth as related to root architecture and availability of N and P. Mycorrhiza. 21: 173-81.

43. Zhang, Sh., Wang, L., MA, F., and Zhang, X. 2015. Can arbuscular mycorrhiza and fertilizer management reduce phosphorus runoff from paddy fields? Journal of Environmental Sciences (33): 211-218.

44. Ziane, H., Hamza, A.M., Beddiar, A., and Gianinazzi, S. 2017. Effects of arbuscular mycorrhizal fungi and fertilization levels on industrial tomato growth and production. International Journal of Agriculture and Biology 19(2): 341-347