Detection of Cauliflower mosaic virus by Serological and Molecular Methods in Northern and Razavi Khorasan Provinces of Iran

پژوهش های حفاظت گیاهان ایران

Article 2, Volume 29, Issue 1 - Serial Number 30, March 2015, Pages 9-15   

Document Type: Research Article

DOI: 10.22067/jpp.v29i1.48536

Authors

A. Entezari^* ; B. Jafarpour; M. Mehrvar

Ferdowsi University of Mashhad

Abstract

Cauliflower mosaic virus is a type member of Caulimovirus from Caulimoviridae family that has double stranded DNA genome and is one of the most destructive viruses of cruciferous plants. In order to detect cauliflower mosaic virus in some areas of Iran under the cultivation of cauliflower, 343 symptomatic leaf samples of cauliflower were collected from Northern and Razavi Khorasan provinces of Iran in summer 2009-2010.To detect CaMV infection, collected plants were tested by DAS-ELISA. Polymerase chain reaction (PCR) using CaMV-specific primers (CMcp-F1\CMcp-R1) for ORF IV genes of CaMV was used for confirmation of infection. A 1400 bp fragment were amplified by PCR and then sequenced. Iranian isolates were compared with other isolates of CaMV deposited in the gene bank. The phylogenic trees of the coat protein genes of CaMV were drown by MEGA5 software using neighbor joining method. The results showed that the Iranian isolates clustered with Chinese and Hungarian isolates in a different clade from North- American isolates.

Keywords

Cauliflower mosaic virus; Coat protein; DAS-ELISA; PCR

References

جعفرپور ب. و جعفرپور ب. 1382. ویروس‌شناسی گیاهی کاربردی (ترجمه). انتشارات دانشگاه فردوسی مشهد. صفحه 87 و صفحات 309-308.

2-Agama K., Beach S., Schoelz J., and Leisner S.M. 2002. The 5' third of Cauliflower mosaic virus gene VI conditions resistance breakage in Arabidopsis Ecotype Tsu-0. Phytopathology, 92:190-196.

3-Altschul S.F., Madden T.L., Schaffer A.A., Zhang J., Zhang Z., Miller W., and Lipman J. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic acids Res, 25: 3389-3402.

4-Buchen-Osmond C. 2006. Cauliflower mosaic virus: Description. CMI/AAB USA. www.ictvdb.org/ICTVdB/ 00.015.0.01.003.htm, visited: 2010/11/7.

5-Cecchini E., Al-Kaff N.S., Bannister A., Giannakou M.E., McCallum D.G.,Maule A.J., Milner J.J., and Covey S.N. 1998. Pathogenic interactions between variants of cauliflower mosaic virus and Arabidopsis thaliana. Journal of Experimental Botany, 321(49):731-737.

6-Chenault K.D., and Melcher U. 1994. Phylogenetic relationships reveal recombination among isolates of Cauliflower mosaic virus. Journal of molecular evolution, 39:496-505.

7-Cheng R.H., Olson N.H., and Baker T.S. 1992. Cauliflower Mosaic Virus: A 420 Subunit (T = 7), Multilayer Structure. Virology, 186: 655-668.

8-Farzadfar Sh., Ahoonmanesh A., Mosahebi G.H., Ohshima K., Koohi-Habibi M., Pourrahim R., and Golnaraghi A.R. 2007. Partial biological and molecular characterization of Cauliflower mosaic virus isolates in Iran. Plant pathology journal, 6(4): 291-298.

9-Franck A., Guilley H., Jonard G., Richards K., and Hirth L. 1980. Nucleotide sequence of Cauliflower mosaic virus DNA. Cell , 21: 285-294.

10-Hoh F., Uzest M., Drucker M., Plisson-Chastang C., Bron P., Blanc S., and Dumas Ch. 2010. Structural Insights into the Molecular Mechanisms of Cauliflower Mosaic Virus Transmission by Its Insect Vector. Jornal of virology, 84(9)p: 4706–4713.

11-Leisner S.M., Neher D.A. 2002. Third Position Codon Composition Suggests Two Classes of Genes Within the Cauliflower Mosaic Virus Genome. J. theor. Biol, 217:195-201.

12-Pappu H.R., and Druffel K.L. 2009. Use of conserved genomic regions and degenerate primers in a PCR-based assay for the detection of members of the genus Caulimovirus. Journal of virological methods, 157:102-104.

13-Qiu S.G., and Schoelz J.E. 1992. Three Regions of Cauliflower Mosaic Virus Strain W260 are Involved in Systemic Infection of Solanaceous Hosts. Virology, 190:773-782.

14-Raikhy, G., Hallan, V., Kulshrestha, S., and Zaidia, A. 2007. Polyclonal antibodies to the coat protein of Carnation etched ring virus expressed in bacterial system: Production & use in immunodiagnosis. Phtopathology, 155:616-622.

15-Saunders K., Lucy A.P., and Covey S.N. 1990. Susceptibility of Brassica species to cauliflower mosaic virus infection is related to a specific stage in the virus multiplication cycle. Journal of General Virology, 71: 1641-1647.

16-Shahraeen N. 2012. An overview of oilseed rape (canola) virus diseases in Iran. International Research Journal of Microbiology, 3(1):024-028.

17-Spence N.J., Phiri N.A., Hughes S.L., Mwaniki A., Simons S., Oduor G., Chacha D., Kuria A., Ndirangu S., Kibata G.N., and Marris G.C. 2007. Economic impact of Turnip mosaic virus, Cauliflower mosaic virus and Beet mosaic virus in three Kenyan vegetables. Plant pathology, 56: 317-323.

18-Woolston C.J., Covey S.N., Penswick J.R., and Davies J.W. Aphid transmission polypeptide are specified by a defined region of the Cauliflower mosaic virus genome. Gene, 23: 15-23.