Survey on Chitinase Production by Several Isolates of Trichoderama and its Biological Control effect on Tomato Root-knot Nematode Meloidogyne javanica

پژوهش های حفاظت گیاهان ایران

Article 9, Volume 29, Issue 1 - Serial Number 27, March 2015, Pages 123-133   

Document Type: Research Article

DOI: 10.22067/jpp.v29i1.32918

Authors

M. Kavari^* ; E. Mahdikhani Moghadam; H. Rouhani

Ferdowsi University of Mashhad

Abstract

Chitin is one of the most important compounds of true fungi cell wall. This compound is used greatly in structural of nematodes egg shell including Meloidogyne javanica. After chitin dissection by chitinase enzyme, convert to its sub units N-Acetyl Glucosamine. Species of Trichoderama genus due to high levels production of many hydrolytic enzymes including Chitinase, Protease and B-1,3-Glucanases, is used as a biocontrol agent of plant pathogens. In the current study, chitinase activity levels of 15 isolates of Trichoderama spp. was investigated to their biocontrol effects on tomato root knot nematode. Among this 15 isolates, T.BI, T6 and T65 respectively with 19.2, 18.3 and 17 U/ml enzyme activity were known as the most active isolates. Also T16, T12N and T12 respectively with 5.5, 5.4 and 3.7 U/ml enzyme activity were known as the weakest isolates. These results was in agreement with the results of greenhouse experiments and T.BI, T65 and T6 isolates were reported as the highest effective in biological control of m.javanica.

Keywords

Trichodema; Biological control; Enzyme Activity; Tomato root-knot nematode

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