Molecular Identification of Turnip Mosaic virus (TuMV) in Hoary Mustard (Herschfeldia incana) From Iran

پژوهش های حفاظت گیاهان ایران

Article 7, Volume 29, Issue 2 - Serial Number 31, July 2015, Pages 231-237   

Document Type: Research Article

DOI: 10.22067/jpp.v29i2.31558

Authors

M. A. Sabokkhiz^{* 1}; B. Jafarpour¹; F. Shahriari Ahmadi¹; S. Tarighi¹; M.R. Safarnejad²

¹Ferdowsi University of Mashhad

²Iranian Research Institute of Plant Protection

Abstract

Turnip mosaic virus (TuMV) is amember of the Potyvirus genus within the Potyviridae family and is one the most important viruses infecting Brassicaceae plants. In April 2012, suspicious symptoms of a viral disease such as mosaic, stunting and malformation were observed on Herschfeldia incana. The collected samples were tested using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers corresponding to TuMV coat protein gene. Amplified fragment (986bp) was first purified and then directly sequenced. Analysis of its CP nucleotide and amino acid sequence revealed 85.42-89.58 % and 91.64-95.12% similarity to those of 31 TuMV isolates from other countries respectively. Phylogenetic tree was constructed by MEGA6 software using neighbor joining method. The results showed that the TuMV isolate and 3 Iranian isolates have been clustered into the basal-B group.

Keywords

Hirschfeldia incana (L.) Lagr.-Foss; molecular identification; Phylogenetic analysis; Turnip mosaic virus (TuMV)

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