The Effect of Thyme and Savory Essential Oils on Quality and Vase life of Cut Narcissus Flowers (Narcissus tazetta L. cv. Shahla)

علوم باغبانی

Article 3, Volume 30, Issue 3, September 2016, Pages 574-580   

Document Type: Research Article

DOI: 10.22067/jhorts4.v30i3.50097

Authors

Iman Baninaeim^{* 1}; Davood Samsampoor²

¹Hormozgan University

²1Department of Horticulture Sciences, Faculty of Agriculture and Natural Resource, University of Hormozgan, Bandar Abbas, Iran

Abstract

Introduction: Narcissus is a genus of hardy, spring-blooming, bulbous plants in the family Amaryllidaceae. Numerous studies have demonstrated positive effects of various chemical additives (e.g. biocides, surfactants, ethylene inhibitors, wound healing enzyme inhibitors) on the postharvest, water relations and longevity of cut flowers. Cut flowers can have limited commercial value because of their dehydrating during vase life that decreased water uptake. Petal senescence is part of a developmental continuum in cut flowers and proceeded by tissue differentiation, growth and development of seeds and coordinated by plant hormones. Senescence can be studied at cellular, tissue, organ or organization level as a genetically programmed event. The vase life of cut narcissus flowers is often very short. The development of senescence symptoms is caused by vascular occlusion, which inhibits water supply to the flowers. Petal senescence was marked by the loss of turgor in petal tissue followed by complete wilting. The development of occlusions is thought to be caused by various factors, such as bacteria, air emboli and physiological responses of stems to cutting. However, despite anecdotal evidence of positive effects, improving postharvest water relations of cut flowers by various physical stem-end treatments is little researched.
Materials and Methods: The Narcissus flowers harvested from Khafr city of Fars province, in February 2015. The Thyme plants harvested in September 2014 and Savory plants harvested in December 2014 and then submitted to hydrodistillation in a Clevenger-type apparatus for 3 hours. This study was carried out in a completely randomized design with 3 replications. The treatments included control (distilled water), two levels of Savory essential oil (50 and 100 ppm) and two levels of Thyme essential oil (100 and 200 ppm). 2% sucrose were added to control and other of treatments . The cut flowers were also kept at temperature of 20±2 ºC with air humidity (RH) of 70±5%. Different parameters including flower weight, uptake of preservative solution, and wilting of flowers were measured every 2 days (0, 2, 4, 6 and 8) and antioxidant enzyme activity (APX) were measured in days of 0, 4 and 8 during storage period, and at the end of experiment, the vase life were measured. The results analyzed by SAS software and drawing tables and diagrams done by Excel software.
Results and Discussion: The results showed that Savory essential oil 50 ppm treatment was effective in improving the flower weight of cut narcissus by increasing uptake of preservative solution and also reducing wilting of flowers and antioxidant enzyme activities. At 200 ppm of thyme essential oil observed reductionin flower weight and uptake of preservative solution, but increasing in wilting of flowers and antioxidant enzyme activities. At 50 ppm of savory essential oil with average days 12.26, the vase life of cut flowers increased significantly as compared to control (10.36) and at 200 ppm of thyme essential oil treatment with average days 8.53, observed decrease invase life of cut flowers compared to control. Impaired water uptake is typically caused by cut stem occlusions due to microbial, physiological and physical plugging of xylem vessels. Essential oils constituents and derivatives have a long history of application as antimicrobial agents in areas of food preservation and medicinal antimicrobial production. physical treatments damage xylem vessels, allow increase of microbes and increase nutrient supply for microbes, which occlude stems. Recently many works carried out about application essential oils as antimicrobial agents under in vitro and in vivo condition and indicated that essential oils could increase postharvest quality of many horticultural crops such as tomato, table grape and kiwifruit (1, 15 and 24). In addition to improving water uptake, other approaches to maintaining a positive postharvest water balance for cut flowers and foliage include minimizing water loss though reduction in leaf area, keeping them in an environment conducive to less water loss (viz. low temperature and high RH) and providing compatible osmotica (e.g. sucrose) in vase and/or pulsing solutions. The loss of membrane integrity has been shown to cause an increase in the permeability and leakage during senescence in various flowers.
Conclusions: Results of this study showed that savory essential oil treatments increased quality and vase life of narssisus cut flowers compared to control At higher concentrations of thyme (200 ppm) the flowers remained closed, wilted quickly and senesced before controls.

Keywords

Ascorbate peroxidase; Catalase; Fresh weight

References

1. Abdolahi, A., Hassani, A., Ghosta, Y., Bernousi, I., Meshkatalsadat, MH. 2010. In vitro efficacy of four plant essential oils against Botrytis cinerea Pers. Fr. and Mucor piriformis A. Fischer. Journal of Essential Oil Bear Plants, 13: 97-107.

2. Amini, Z., Haddad, R., and Moradi, F. 2008. Effect of Water stress on Actions of Antioxident enzymes Reproductive growth stages of Barley (Hordeum vulgare L.). Science and Technology of Agriculture and Natural Resources, 46:65-74. (in Persian)

3. Armitage, M.A. 2003. Speciality cut flower. Timber Press, 586.

4. Bayat, H., Azizi, M., Shoor, M., Vahdati, N. 2010. Effect of ethanol and essential oils on extending vase life of carnation cut flower (Dianthus caryophyllus cv. yellow candy). Fifth National Conference New Ideas in Agriculture. (in Persian with English abstract)

5. Bleeksma, H.C., and Van Doorn, W.G. 2003. Embolism in rose stems as a result of vascular occlusion by bacteria. Postharvest Biology. Technology, 29:334-340.

6. Cavar, S., Maksimovic, M., Solic, M., Jerkovic- Mujkic, A., Besta, R. 2008. Chemical composition and antioxidant and antimicrobial activity of two Satureja essential oils. Food Chemistry, 111: 648-653.

7. Chamani, A., Khalighi, A., Joise, D., Irongh, D., Zamani, Z., Mostofi, Y., and Kafie, M. 2005. Effect of Silver thiosolfat and 1- MCP on Physicochemical properties cut rose flowers "Frest red". Jornal of Science and Technology of Agriculture. 3:159-170. (in Persian)

8. Gulluce, M., Sokmen, M., Daferera, D., Agyar, G., Ozkan, H., Kartal, N., et al. 2003. In vitro antibacterial, antifungal and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agriculture Food Chemistry, 51: 3958-3965.

9. Hasanpoor asil, M., Alikarimi, H., and Musanejad, S. 2014. Effect of Essential oils, Silver nanoparticles and Chemicals on Vase life of cut Gladiolus Flowers (Gladiolus grandiflora L.). Iran Science of Agriculture. 4:449-460. (in Persian)

10. He, S., Joyce, D.C., Irving, D.E., and Faragher, J.D. 2006. Stemend blockage in cut Grevillea ‘Crimson Yul-lo’ inflorescences. Postharvest Biology and Technology, 41: 78–84.

11. Hosseini darvishani, S.S., and Chamani A. 2013. Exploring the possibility of improving the shelf life of cut rose flowers "Red old" by Silver nanoparticles and Chemicals. Jornal of Science of Agriculture. Iran. 1:31-41.

12. Ichimura, K., Taguchi, M., and Norikoshi, R. 2006. Extension of the vase life in cut roses by treatment with glucose, isothiazolinonic germicide, citric acid and aluminum sulphate solution. Japan Agricultural Research Quarterly, 40 (3): 263–269.

13. Jalili MR, Hassani, A., Ghosta, Y., Abdollahi, A., Pirzad, A., Sefidkon, F. 2011. Improving postharvest quality of table grape cv 'Rish Baba' using Thymus Kotschyanus and Carum copticum essential oils. Journal of Food Safety, 31: 132-139.

14. Kulisic, T., Radonic, A., Katalinic, V. 2004. Use of different methods for testing antioxidative of oregano essntial oil. Food Chemistry, 85: 633 -640.

15. Mortazavi, S.N., Naderi, R., Khalighi, A., Babalar, M., and Allizadeh, H. 2007. The effect of Cytokinin and Calcium on cut flower quality in Rose (Rosa hybrida cv. Illona). Journal of Food Agriculture and Environment, 5:3 & 4:1459-0263.

16. Nakano, Y., and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22: 867–880.

17. Shahnia, M., Khaksar, R. 2013. Antimicrobial effects and determination of minimum inhibitory concentration (MIC) methods of essential oils against pathogenic bacteria. Iranian Journal of Nutrition Sciences & Food Technology. Vol. 7, 5: 949-955. (in Persian with English abstract)

18. Shahsavari, N., Barzegar, M., Sahari, M., Naghdibadi, H. 2008. Antioxidant activity and chemical characterization of essential oil of Bunium persicum. Plant Food Hum Nutr, 63: 183-188.

19. Sharma, P., and Dubey, R.S. 2005. Drought induces oxidative stress and enhances the activities of antioxidant enzymes in growing rice seedlings. Plant Growth Regulation, 46: 209-221.

20. Shirzad, H., Hassani, A., Ghosta, Y., Abdollahi, A., Finidokht, R., Meshkatalsadat, M.H. 2011. Investigation the possibility use of natural plant extracted compounds to reduce postharvest gray mould (Botrytis cinerea Pers. Fr.) of kiwifruits (Actinidia deliciosa) during storage. Journal of Plant Protect Research, 51: 1-6.

21. Sobhani, M.A. 2000. Effect of Chemical treatments on Extending Vase life of cut Chrysanthemums flowers. A Thessis M.Sc. Gilan University.

22. Souri, E., Amin, G., Dehmobed-Sharifabadi, A., Nazifi, A., Farsam, H. 2004. Antioxidative activity of sixty plants from Iran. Iranian Journal of Phars Research, 3(1): 55-9.

23. Voda, K., Boh, B., Vrta-cnik, M., Pohleven, F. 2003. Effect of the antifungal activity of oxygenated aromatic essential oil compounds on the whiterot Trametes versicolor and the brown-rot Coniophora puteana. International. Biodeter. Biodegr., 51: 51-59.

24. Ye, Z., Rodriguez, R., and Tran, A. 2000. The development transition of flowering represses ascorbate peroxidase activity and induces enzymatic lipid peroxidation in leaf tissue in Arabidopsis thaliana. Plant science, 158: 115-127.