تأثیر سطوح مختلف تفاله زیتون بر فراسنجه‌های تخمیری، مقدار آنزیم‌های سلولازی وتولید پروتئین میکروبی شکمبه بره‌های نر لری

پژوهشهای علوم دامی ایران

مقاله 3، دوره 9، شماره 4، بهار 1397، صفحه 424-436  

نوع مقاله: علمی پژوهشی - تغذیه نشخوارکنندگان

شناسه دیجیتال (DOI): 10.22067/ijasr.v9i4.56420

نویسندگان

فرانک دوستی ¹؛ تقی قورچی¹؛ اصغر سپهوند²؛ بهروز دستار ¹؛ آرش آذرفر³

¹دانشگاه علوم کشاورزی و منابع طبیعی گرگان

²مرکز تحقیقات داروهای گیاهی رازی، دانشگاه علوم پزشکی لرستان، خرم آباد، ایران

³دانشکدۀ کشاورزی، دانشگاه لرستان، خرم آباد

چکیده

جهت بررسی تأثیر تفاله زیتون جیره غذایی بر فراسنجه‌های شکمبه‌ای، فعالیت آنزیم‌های سلولاز و سنتز پروتئین میکروبی تعداد 15 بره نر لری با میانگین وزن زنده 3 ± 94/27 و میانگین سن سه ماهگی در قالب طرح کاملا تصادفی با سه تیمار و پنج تکرار مورد استفاده قرار گرفتند. هر بره پرواری در قفس انفرادی به مدت 16 روز دوره عادت پذیری و 84 روز دوره پرورش نگهداری شد. تیمارهای آزمایش شامل سطوح صفر، 10 و 20 درصد تفاله زیتون در جیره غذایی بود. جیره‌های آزمایشی از نظر محتوی پروتئین و انرژی برابر بودند. نتایج نشان دادند، استفاده از تفاله زیتون دارای تأثیر معنی‌داری بر فراسنجه‌های شکمبه‌ای، فعالیت آنزیم‌های سلولاز و سنتز پروتئین میکروبی می‌باشد. استفاده از جیره حاوی 10 و 20 درصد تفاله زیتون باعث کاهش معنی‌داری در مقدار pH، غلظت آمونیاک تولیدی، مشتقات پورینی و تولید پروتئین میکروبی در شکمبه نسبت به جیره شاهد شد. فعالیت کل آنزیم کربوکسی متیل سلولاز در جیره با 10 درصد تفاله زیتون و آنزیم میکروکریستالین سلولاز در جیره با 20 درصد تفاله زیتون افزایش یافت. با توجه به نتایج این آزمایش، به نظر می‌رسد استفاده از تفاله زیتون تا سطح 20 درصد خوراک بره‌های پرواری می‌تواند موجب کاهش pH و غلظت آمونیاک شیرابه شکمبه و از طرفی افزایش فعالیت آنزیم‌های سلولتیک و پروتئین عبوری از شکمبه شود.

کلیدواژه‌ها

کربوکسی متیل سلولاز؛ مشتقات پورینی؛ میکروکریستالین سلولاز

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