مقایسه روش های استخراج آر.آن.ای برای شناسائی ویروس برگ بادبزنی مو

پژوهش های حفاظت گیاهان ایران

مقاله 14، دوره 30، شماره 1، خرداد 1395، صفحه 127-133   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v30i1.40075

نویسندگان

زهرا غلامپور؛ محمد زکی عقل^*

فردوسی

چکیده

وجود مقادیر زیاد ترکیبات فنلی و پلی ساکاریدها در بافتهای مو از معضلات استخراج اسید نوکلئیک با کیفیت بالا از این گیاه است. به منظور ردیابی موثر ویروس برگ بادبزنی مو در تاکهای آلوده چهار روش استخراج RNA با یکدیگر مقایسه و روش استخراج اسید نوکلئیک از این گیاه بهینه سازی شد. کیفیت اسید نوکلئیک استخراج شده به روشهای اسپکتروفوتومتری و واکنش زنجیره ای پلی مراز با نسخه برداری معکوس تعیین شد. در بین روشهای مورد مقایسه اختلاف زیادی در نتایج مشاهده شد به نحوی که بعضی از این روشها قادر به شناسائی ویروس در بافت آلوده نبودند. در بین روشهای مورد بررسی، استفاده از CTAB-PVPP در بافر استخراج همواره بیشترین غلظت و بالاترین کیفیت اسید نوکلئیک را در پی داشت و ردیابی ویروس در اغلب نمونه ها امکان پذیر بود.

کلیدواژه‌ها

استخراج نوکلئیک اسید؛ مقایسه؛ ویروس برگ بادبزنی مو

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