شناسایی و بررسی مولکولی ویروس موزاییک معمولی لوبیا (BCMV) و موزاییک نکروتیک معمولی لوبیا (BCMNV) در استان مازندران

پژوهش های حفاظت گیاهان ایران

مقاله 16، دوره 30، شماره 1 - شماره پیاپی 34، فروردین 1395، صفحه 143-150   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v30i1.41631

نویسندگان

زهره مرادی¹؛ محسن مهرور^{* 1}؛ احسان نظیفی²

¹دانشگاه فردوسی مشهد

²دانشگاه مازندران

چکیده

طی سال های 1392 و 1393، تعداد 50 نمونه برگ لوبیا دارای علائم از مزارع استان مازندران جمع آوری و RNA کل آنها استخراج شد. طی آزمون RT-PCR با استفاده از یک جفت آغازگر دژنره منطبق بر ژن کدکننده پروتئین Cylindrical inclusion (CI) قطعه ای به طول bp680 در 13 نمونه تکثیر گردید. تکثیر اختصاصی نمونه های آلوده با آغازگرهای CIF/R، نشان از آلودگی به یک پوتی ویروس داشت. محصول PCR مربوط به دو جدایه پس از همسانه سازی در حاملpTG19-T ، توالی یابی شد. آنالیز بلاست توالی ها نشان داد که قطعات تکثیر شده در واکنش PCR متعلق به ویروس موزاییک نکروتیک معمولی لوبیا (Bean common mosaic necrosis virus, BCMNV) (از لوبیا سفید) و ویروس موزاییک معمولی لوبیا (Bean common mosaic virus, BCMV) (از لوبیا چشم بلبلی) است. آنالیز فیلوژنتیکی جدایه های تعیین ترادف شده در ناحیه ژنی CI نشان داد که جدایه ی تعیین توالی شده BCMV-MAZ (از ساری) در سطح نوکلئوتیدی و اسید آمینه ای به ترتیب دارای بیشترین شباهت (8/96% و 7/98%) با جدایه آمریکا (RU1M) و کمترین شباهت (5/79% و 6/91 %) با جدایه کره جنوبی (Habin1) می باشد. همچنین جدایه BCMNV-MAZ (از جویبار) بیشترین شباهت (8/97%) را با جدایه های آمریکایی NL-3 K،NL5 و کمترین شباهت (9/96%) را با NL8 در سطح نوکلئوتیدی دارد. در سطح اسید آمینه ای نیز 7/97 % شباهت با سایر جدایه های مورد بررسی دارد. این اولین گزارش از وجود ویروس های مذکور در استان مازندران به روش مولکولی است.

کلیدواژه‌ها

BCMV؛ BCMNV؛ RT-PCR؛ آنالیز فیلوژنتیک

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