The Effect of Temperature and Water Potential on Seed Germination of Asian spiderflower (Cleome viscose L.): As Invasive Weed in Soybean Fields in Golestan Province

پژوهش های حفاظت گیاهان ایران

Article 4, Volume 30, Issue 2 - Serial Number 35, June 2016, Pages 292-303   

Document Type: Research Article

DOI: 10.22067/jpp.v30i2.44481

Authors

M. Shirdel¹; Asieh siahmarguee^{* 2}; Masomeh Yones-Abadi³

¹Islamic Azad University of Gorgan

²Gorgan University of Agricultural Sciences and Natural Resources

³Agricultural Research and Natural Resources Center of Golestan Province

Abstract

Introduction: Cleome viscose Linn. with a common name as “Asian spiderflower”, belongs to the Capparidacea family. It is an annual, sticky herb found as a common weed all over the tropical regions of the world. It is a very competitive weed of annual crops. This plant is currently introduced as an invasive plant in soybean fields of Golestan province. There is no management recommendation to control Asian spider flower. Thus large quantities of soybean destroyed by this weed every year. Germination and emergence are the two most important stages in the life cycle of plants. Environmental factors such as temperature, light, pH, planting depth and soil moisture are known to affect seed germination and emergence of weeds. An understanding of the germination biology of Asian spider flower would facilitate the development of better management strategies for this weed. Therefore, the purposes of this research were to study the effects of temperature and water potential on Asian spider flower seed germination.
Material and Methods: To evaluate the effect of temperature and water potential on seed germination and determination of seed germination cardinal temperatures of Asian spider flower, an experiment was conducted as Factorial Experiment in Completely Randomized Design with 4 replications in Agricultural Research and Natural Resources Center of Golestan Province during 2013. Treatments were included temperatures with seven levels (15, 20, 25, 30, 35, 38 and 400C) and water potentials with six levels (0, -2, -4, -6, -8 and -10 bar). Germination was monitored daily until germination discontinued and the number of the germinated seeds was recorded. Seeds were observed twice daily and considered germinated when the radical was approximately >2mm long. To quantify the response of germination rate to temperature and to determine the cardinal temperatures for germination original beta, and modified beta, segmented and dent models were used. Water potential data showed a sigmoid trend and a three-parameter logistic model was fitted to data (Equation 1).
Y=Gmax/ [1+(X/X50)b]
where, G is the total germination (%) at concentration x, Gmax is the maximum germination (%), x50 is the osmotic potential required for 50% inhibition of the maximum germination and Grate indicates the slope of the curve in x50. Statistical Analysis System (SAS) was used for analyzing the data.
Results and Discussion: Results showed temperature, water potential and interaction between them significantly affected germination percentage and germination rate, the time required to reach 5% (D05), 10% (D10), 50% (D50), 90%(D90) and 95% (D95) seed germination of Asian spiderflower. The results showed that by increasing the temperature to 30 °c, seed percentage and germination rate increased and then decreased. Water potential reductions declined seed germination percentage and germination rate. Comparing different models to determine seed germination cardinal temperatures, indicated that the segmented model described germination rate changes to temperature better that the others. Based on the output of this model, germination base, optimum and ceiling temperatures of the plant were 15.46, 33.21 and 39.64 0C, respectively. The result revealed that increasing the PEG concentration, increased the base temperature and decreased optimum and ceiling temperatures. The Logistic model fitted with germination percentage of seeds in different water potentials to different temperatures. Parameters of the model showed the maximum seed germination percentage occurred in the 25 to 35 0C. Highest and lowest X50 parameter (potential required for 50% inhibition of the maximum germination) observed at 30 and 38 0C, respectively. The results showed that the germination ability of Asian spiderflower occurred at less potentials water in the optimum temperature range.
Conclusion: The results showed that high temperatures were required for seed germination of Asian spider flower. Although water potential has reduced the germination of the weed, we can say this is somewhat drought tolerant plants. Based on the segmented model, cardinal temperature for germination, including base, optimum and ceiling temperatures of the plant were 15.46, 33.21 and 39.64 0C, respectively. Check the seed germination, percentage changes in water potentials at different temperatures showed that the desired temperature the plant's ability to withstand in adverse environmental condition increases. Results of this experiment can be concluded that environmental factors affected the germination of Asian spider flower, and this information could help to predict the spread of Asian spider flower in new areas.

Keywords

Asian spiderflower; Invasive plant; Soybean; temperature; Water potential

References

1- Alimagham S.M., and Ghaderifar F. 2011. Effects of temperature and salinity on the germination components of king officer (Melilotus officinalis). The second Conference on Science and Seed Technology. Islamic Azad University, Branch of Mashhad. (in Persian)

2- Azarvan H. 2011. Time and dose effects of the herbicide Bentazon application on weed management of beans. M.S.C thesis, Islamic Azad University, Takestan branch. (in Persian)

3- Baigi Z., Jafarnezhad A., and Alimoradi L. 2013. Effect of water potential and temperature levels on germination characteristics of rye (Secale cereal L.). The 5th Iranian Weed Science Congress. Tehran University. Pp: 101-104. (in Persian)

4- Chauhan B.S., Gill G., and Preston C. 2006. Factors affecting seed germination of threehorn bedstraw (Galium tricornutum). Weed Science, 54: 471_477.

5- Chauhan B.S., GILL G., and Preston C. 2006. Factors affecting seed germination of annual sowthistle (Sonchus olevaceus) in southern Australia. Weed Science, 54: 854-860.

6- Edalat M., and Kazemeini S.A. 2013. Estimating cardinal temperatures of tumble mustard (Sisymbrium altissimum) and slender foxtail (Alopecurus myosuroides) seed germinations. The 5th Iranian Weed Science Congress. Tehran University. Pp: 280-283. (in Persian)

7- Ghaderifar A., Alimagham S.M., Rezaee Moghadam H.V., and Haghighi M. 2012. Effects of environmental factors on germination and emergence of rye crops in wheat fields as wild plant. Electronic Journal of Crop Production, 5(4): 133-121. (in Persian with English abstract)

8- Golmohammadzadeh S., Zaefarian F., and Rezvani M. 2013. Effects of burial depths, temperature and light on two papaver species germination. The 5th Iranian Weed Science Congress. Tehran University. Pp: 186-189. (in Persian)

9- Jami-Al_Ahmadi M., and Kafi M. 2007. Cardinal temperature for germination of Kochia scoparia (L). Journal of Arid Environments, 68: 308-314. (in Persian with English abstract)

10- Jansen P.C.M. 2004. Cleome Viscosa L. Internet Record From Protabase. Grubben, G.J.H. & Denton, O.A. (Editors). PROTA (Plant Resources Of Tropical Africa / Ressources Vegetales De l’Afrique Tropicale), Wageningen, Netherlands. (28 April 2008).

11- Kamkar B., Koochaki A., Nassiri Mahallati M., and Rezvani-Moghaddam P. 2006. Cardinal temperatures for germination in three millet species (Panicum miliaceum, Pennisetum glaucum and Setaria italic). Asian Journal Plant Science, 5 (2): 316-319.

12- Kazerooni-Monfared A., Takasi S., Banaeeian M., Ghanbari A., Rahimian Mashadi M., and Pernilson K. 2013. Effects of temperature and osmotic potential on the seed germination of Orobanche aegyptiaca. Journal of Applied Research of Plant Ecophysiology, 1(1): 33-50. (in Persian with English abstract)

13- Kebreab E., and Murdoch A.J. 2000. The effect of water stress on the temperature range for germination of Orobanche saegyptiaca seeds. Seed Science Research, 10: 127-133.

14- Michel B.E., and Kaufmann M.R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51: 914-916.

15- Mighani F. 2003. Allelopathy, from Concept to Application. Parto Publication. 256 p.

16- Minbashi Moini M., Rahimiyan H., Zand A., and Baghestani M. 2010. Invasion weeds, a forgotten challenge. The 3rd Iranian Weed Science Congress, Babolsar. February 2010. (in Persian)

17- Menon A., and Kulkarni A.R 1987. Ecological studies in Cleome viscosa L. seed and seed germination. Indian Botanical Reporter. 6 (1): 1-7

18- Nameni H. 2013. Effect of nitrogen and phosphorus supply on yield phonology and yield components of soybean ketool cultivar in East region of Golestan province. Msc thesis, Islamic Azad University, Branch of Gorgan.120p. (in Persian)

19- Nejadhasan B. 2014. Effect of some environmental factors on seed germination of Arugula (Eruca sativa Mill). Msc Thesis on Agronomy, Gorgan University of Agricultural Science and Natural Resources. 102 p. (in Persian)

20- Peters N.‌C.B., Atkims H.‌A., and Brain P. 2000. Evidence of differences in seed dormancy among populations of (Bromus sterilis). Weed Research, 40: 467-478.

21- Rahimi Z., and Kafi M. 2009. Effect of different levels of drought on germination characteristics of Purslane (Portulaca oleracea L.). Journal of environmental stresses in Agricultural Science, 2(1): 87-91. (in Persian with English abstract)

22- Rashed-Mohasel M.H., Kazeroni-Monfared A., and Al-Ebrahim M.T. 2009. Effects of some environmental factors on the germination of wild lettuce weed (Lactuca serriola). Journal of Plant Protection (Science and Agricultural Resources), 25(4): 350-341. (in Persian with English abstract)

23- Savari-nejad A.R., Younesabadi M., and Habibian L. 2010. Determination of the importance weeds in soybean field emphasising on invasive plants in Golestan province. 19th Plant Protection Congress of Iran. p 10. (in Persian)

24- Sohrabi S., Ghanbari A., Rashed Mohasel M.H., Nasiri Mahalati M., and Gharekhlou J. 2011. Effect of temperature, drought and salinity on the seed germination of invasive weed wildmelons (Cucumis melo). The 4th weed science congress of Iran. Khozestan, Pp: 259-261. (in Persian)

25- Soltani A., and Madah V. 2009. Simple Applied Programs for Education and Research in Agriculture. Niac Press Publication. 80 p. (in Persian)

26- Soltani A., Galeshi S., Zeinali E., and Latifi N. 2002. Germination, seed reserve utilization and seedling growth of chickpea as affected by salinity and seed size. Seed Science and Technology. 30: 51-60.

27- Soltani A., Ghaderifar F., and Soltani A. 2007. Modeling application of germination in response to temperature and water potential on seed science researches. The first Conference on Science and Seed Technology. Gorgan University of Agricultural Sciences and Natural Resources. (in Persian)

28- Soltani A., Robertson M.J., Trabi B., Yousefi M., and Sarparast R. 2006. Modeling seedling emergence in chickpea as affected by temperature and sowing depth. Agricultural Forest Meteorology. 138: 156-167.

29- Taassob-Shirazi M., Forouzesh S., Zare A., and Rahimian-Mashhsdi H. 2013. Germination phonology of invasive plant (Amsinckia menziesii). The 5th Iranian Weed Science Congress. Tehran University. Pp: 448-451. (in Persian)

30- Takasi S., Al-Ebrahim M.T., Kazeroni-Monfared A., and Rashed Mohasel M.H. 2009. Effect of temperature, light, flooding and plant in depth on germination percentage of wildlettuce weed (Lactuca serriola). The 3th Weed Science Congress, Babolsar. Pp: 33-36. (in Persian)

31- Takim F.O., and Fadayomi O. 2010. Influence of tillage and cropping systems on field emergence, growth of weeds and yield of maize (Zea mays L.) and cowpea (Vigna unguiculata L.). Austalian Journal Of Agricalture engineerng . 1 (4): 141-148.

32- Torkamani A. 2015. Investigation the effects of salinity, flooding, planting depth, pH and interaction between temperature and salinity on seed germination of Asian spiderflower (Cleome viscosa L.). M.sc thesis of weed science, Islamic Azad University, Gorgan Branch. 91p. (in Persian)

33- Zahed S., Gherekhloo J., and Bagherany N. 2013. Prostrate spurge seed germination response to salinity and drought caused by various concentrations of poly ethylene glycol 6000. The 5th Iranian Weed Science Congress. Tehran University. Pp: 476-479. (in Persian)