Discrimination of Species and Hybrid Detection in Myriophyllum Spp.: an Introduction to Biodiversity Conservation and Invasion Avoidance

پژوهشهای زراعی ایران

Article 6, Volume 11, Issue 4 - Serial Number 32, January 2014, Pages 583-592   

Document Type: Research Article

DOI: 10.22067/gsc.v11i4.32655

Authors

R Ghahramanzadeh^* ; S. H Marashi; S Malekzadeh Shafaroudi; R Smulders; C Van De Wiel; F Shahriari

Abstract

Minimizing economical loss through introduction of invasive alien species (IAS) in local ecosystem is one of the most important issues in biosecurity. The hybridization potential between non-indigenous and native species has raised concerns due mainly to introgression, which can cause extirpation of native species through gene contamination. In the present study, 71 samples belonging to 12 species from Myriophyllum genus were assessed in Plant Breeding group of Wageningen University. Internal transcribed spacer (ITS) was used for identification of invasive species from related native and possible hybrid plants. The result showed that based on universal application, high sequence divergence and species discrimination, ITS is a powerful sequence for the identification of invasive species from related non-invasive foreign and native species. In contrast to morphological data, ITS grouped suspected hybrid plants in to M. heterophyllum and demonstrated that they have not resulted from hybridization. These observations suggest that multiple introduction and genetic recombination among different introduced genotypes or genetic pools could be reasons of non-flowering in suspected hybrid plants. Results showed that molecular markers enable to distinguish invasive plant species from their most closely related congeners. This could be helpful with enforcing a ban on important of such invasive which can help to plant ecosystem and biodiversity stability.

Keywords

Biosecurity; Ecosystem stability; Invasive species; Hybridization