بررسی فنوتیپی و ژنوتیپی جدایه های Pseudomonas syringae pv. syringae عامل بلاست مرکبات در غرب مازندران و شرق گیلان

پژوهش های حفاظت گیاهان ایران

مقاله 1، دوره 30، شماره 3 - شماره پیاپی 36، مهر 1395، صفحه 359-367   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v30i3.30987

نویسندگان

سمانه سمیعی شیرکده¹؛ مرتضی گل محمدی^{* 2}؛ سید علی الهی نیا¹؛ سمانه بشیری³

¹دانشگاه گیلان

²وهشکده مرکبات و میوه‌های نیمه گرمسیری

³دانشگاه کردستان

چکیده

بیماری بلاست یکی از بیماری های شایع در مناطق گرمسیری و نیمه گرمسیری کشت مرکبات از جمله شمال ایران می باشد که عمدتاً به-وسیله جدایه های باکتری (Pss) Pseudomonas syringae pv. syringae ایجاد می شود. به منظور بررسی فنوتیپی و ژنوتیپی جدایه های Pss عامل بیماری، نمونه برداری از درختان مرکبات در غرب مازندران و شرق گیلان طی ماه های اسفند 1390 تا اوایل خرداد 1391 صورت گرفت. جدایه ها از نظر خصوصیات فنوتیپی، بیماری زایی مورد بررسی قرار گرفتند که تعداد 27 جدایه باکتری از بافت های آلوده بر اساس آزمون های فنوتیپی و بیماری زایی به‌عنوان Pss شناسایی و جدا شدند. جهت تأیید شناسایی جنس و گونه باکتری از روش مولکولی (PCR) با استفاده از جفت آغازگرهای اختصاصی باکتری مربوط به ژن اختصاصی تولید سیرینگومایسن استفاده گردید. تمامی جدایه ها با جفت آغازگر اختصاصی قطعه قابل انتظار 198 جفت بازی از ژن syrB را تولید کردند. انگشت نگاری ژنومی جدایه ها به روش rep-PCRبا استفاده از آغازگرهای ERIC و REP انجام شد. نتایج به دست آمده از دندروگرام تلفیق نقوش الکتروفورز حاصل از آغازگرهای ERIC و REP نشان داد که جدایه ها در سطح تشابه 75 درصد در 6 گروه قرار گرفتند. علی رغم وجود خصوصیات فنوتیپی مشابه، نتایج بیانگر وجود ناهمگونی از نظر ژنتیکی در بین جدایه های Pss، عامل بلاست مرکبات در غرب مازندران و شرق گیلان می باشد.

کلیدواژه‌ها

انگشت نگاری؛ تنوع ژنتیکی؛ Pss؛ rep-PCR

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