امکان القای مقاومت در گوجه فرنگی علیه Rhizoctonia solani و برخی مکانیسم های آن

پژوهش های حفاظت گیاهان ایران

مقاله 3، دوره 30، شماره 3، آذر 1395، صفحه 441-448   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v30i3.44805

نویسندگان

فریبا نیک رفتار؛ پریسا طاهری^* ؛ سعید طریقی

دانشگاه فردوسی مشهد

چکیده

به منظور بررسی امکان القای مقاومت در گیاه گوجه فرنگی رقم حساس موبیل علیه قارچ Rhizoctonia solani، از ویتامین ها و هموسرین لاکتون ها استفاده شد. تیمار تیامین 20 میلی مولار بهترین تأثیر را در القای مقاومت علیه این بیمارگر داشت. جهت بررسی نقش پراکسیداز در مقاومت القایی ناشی از تیامین، از سدیم آزید به عنوان بازدارنده پراکسیداز استفاده شد و در زمان های 0، 24، 48 و 72 ساعت بعد از مایه زنی قارچ، میزان ترکیبات فنلی در نمونه های تیمار شده و شاهد اندازه گیری شد. بین محتوای فنل کل در نمونه های مختلف و زمان های متفاوت مورد بررسی، تفاوت قابل توجهی مشاهده شد. در نمونه های تیمار شده با سدیم آزید میزان ترکیبات فنلی نسبت به دیگر نمونه ها کمتر بود. جلوگیری از فعالیت این آنزیم منجر به کاهش تولید ترکیبات فنلی و کاهش میزان مقاومت به بیمارگر در برگ های تیمار شده با تیامین و سدیم آزید گردید. بر اساس نتایج این تحقیق، پراکسیداز و ترکیبات فنلی نقش مهمی در مقاومت القایی ناشی از تیامین در گوجه فرنگی علیه R. solani ایفا می کنند.

کلیدواژه‌ها

القای مقاومت؛ پراکسیداز؛ گوجه فرنگی؛ محتوای فنل کل؛ ویتامین B1

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