The Effect of Myrtle (Myrtus communis) Leaves on Digestibility, Some Blood and Rumen Metabolites and Protozoa Morphology in Arabi Sheep

پژوهشهای علوم دامی ایران

Article 4, Volume 10, Issue 3 - Serial Number 35, September 2019, Pages 353-365   

Document Type: Ruminant Nutrition

DOI: 10.22067/ijasr.v10i3.63856

Authors

kolsom salehpour¹; Tahereh Mohammadabadi^{* 2}; Mohammad Reza Ghorbani¹

¹Department of Animal Science, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran

²Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Khuzestan, Iran.

Abstract

Introduction: Myrtle is from Myrtaceae family with scientific name, Myrtus communis. Myrtle leaves mainly containing terpynolen, cineol, linalool, terpineol and acetate lynalyl. Also including the active ingredients as phenolic acids such as galic acid, vanillic acid and ferulic acid, tannins such as galotannin and flavonoids such as, myrcetin, catechin and quercetin. Many plants synthesis secondary metabolites that have antimicrobial activity, regulation of ruminal fermentation and subsequently improve nutrient intake. Information on the use of myrtle leaves in ruminant nutrition is rare, so this experiment was designed to investigate the effect of myrtle leaves on digestion, some blood and rumen parameters and protozoa species in Arabi sheep.
Materials and methods: At first step, the appropriate level of myrtle leaves between the levels 0.2, 0.4 and 0.6 % with diet 70:30 concentrate to forage was determined by in vitro methods (gas test and tilly and terry method). Rumen fluid was collected from sheep before the morning feeding. About 200 mg sample (1.0 mm screen) incubated in 100 ml vials with 35 mL buffered rumen fluid under continuous CO2 reflux for 2, 4, 6, 8, 10, 12, 16, 24, 48, 72 and 96 h, at 39°C. Cumulative gas production data were fitted to the exponential equation Y=B (1−e−ct). Partitioning factor, microbial biomass and truly digested organic matter was calculated. For determination of partitioning factor at the end of each incubation period, the content of vials was transferred into an Erlenmeyer flask, mixed with 20 mL neutral detergent fiber solution, boiled for 1 hour, filtered, dried (in oven at 60 °C for 48 h) and ash. Digestibility of dry matter and NDF of samples were determined using tilly and terry method. Rumen fluid was collected from animals, and were mixed with McDougall buffer in a ratio 1:4. After gasifying with CO2, tubes were incubated at 39 ˚C. After 48 h of fermentation, 6 mL of 20% HCl solution and 5 mL pepsin solution were added and the incubated for 48 h, simulating post-ruminal degradation. After incubation, the residual substrates of each tube were filtered and used to determine digestibility of DM and NDF.
At the second step of experiment, the appropriate amount of myrtle leaves; 0.4 %; was used in feeding 8 Arabi sheep (23±1.5 kg) for 30 days. In the end of experiment, feed intake, digestibility, protozoa morphology, some blood metabolites and rumen parameters were measured. Data were subjected to analysis as a completely randomized design using the General Linear Model (GLM) procedure of SAS. version 9.1. The Duncan multiple range test was used to compare means at P< 0.05.
Results and Discussion: The results of in vitro step showed that the best fermentation, gas production and digestibility were for 0.4% the myrtle leaves. On the base of the result of in vivo step, using of myrtle leaves had not any effect on dry matter and organic matter intake (P>0.05). Treatment containing myrtle leaves had the highest protein intake in compared with control treatment (P<0.05). Digestibility of crude protein and NDF in diet with myrtle in comparison to control significantly increased (P<0.05). But there was no significant difference between the dry matter, organic matter and ADF digestibility of sheep’s (P>0.05). Protozoa population was significantly not affected by inclusion myrtle leaves in the diet (P>0.05). Ammonia levels in diets treated with myrtle (8.29) significantly reduced in compared with the control (16.5) (p<0.05). The myrtle leaves treatment decreased triglyceride and blood glucose of sheeps (P<0.05). Factors such as period of adaptation to medicinal herbs and interaction with other dietary components and their amounts in the diet, can influence the amount of dry matter intake. Essential oils according to chemical structure, resources and activities have different effects on rumen fermentation and animal performance. The negative effect of active ingredients of medicinal plants on ammonia-producing bacteria caused to decrease of rumen ammonia production, also reducing protozoa and swallowing of bacteria that can be other reason for ammonia decrease. Polysaccharides, flavonoids, glycoproteins, polypeptides, steroids, alkaloids and pectin in plants such as myrtle can justify the hypoglycemic properties of these plants. Medicinal plants and their extracts are effective in reducing blood cholesterol and other lipids. It is reported essential oil reduces pH of intestinal tract through production of degrading enzymes of bile, also inhibit the activity of HMG-COA enzyme.
Conclusion: According to the results, it seems 0.4 % myrtle leaves has beneficial effects on digestibility and can reduce blood glucose and triglyceride; therefore, maybe it can be used as an herbal supplement in Arabi sheep diet.

Keywords

Myrtle leaf; Arabi sheep; digestibility; Protozoa; blood factors

References

1. Abarghouei, M. J., and Y. Roozbahan. 2013. The effect of grape pomace on gas production parameters of the rumen protozoa population of rumen fluid, using sheep's. Journal of Animal Science, 44 (4): 384-375.

2. Afshar Hamidi, B., R. Pirmohammadi, H. Mansoori, and M. Fajri. 2013. The effects of adding thymus plant to lactating goats rations on digestibility parmeters and milk yield performance. Animal Sciences Journal (Pajouhesh & Sazandegi),101: 29-36.

3. Aidi Wannes, W., B. Mhamdi., J. Sriti, and B. Marzouk. 2010. Changes in essential oil composition of Tunisian Myrtus communis var. italica during its vegetative cycle. Journal Essential Oil Research, 22: 13-18.

4. Ammar, H., S. Lopez, and J. S. Gonz´alez. 2005. Assessment of the digestibility of some Mediterranean shrubs by in vitro techniques Journal of Animal Feed Science and Technology, 119: 323-331.

5. Ammar, H., A. Z. M. Salem, and S. Lopez. 2010. Impact of PEG 6000 on in vitro gas production kinetics of some Mediterranean shrubs in sheep. In: Porqu eddu C. (ed.), Rios S. (ed.). The contributions of grasslands to the conservation of Mediterranean biodiversity. Zaragoza: CIHEAM / CIBIO / FAO / SEEP, 92: 209 -213.

6. AOAC. 2001. Association of Official Analytical Chemists. Official Methods of Analysis. Washington, D. C. USA.

7. Barazandeh, M. M. 2001. Identification of essential oil components of myrtle (Myrtus communis. L.). Iran journal medicinal Aromatic, 6: 115-127.

8. Benchaar, C., A. V. Chaves, G. R. Fraser, Y. Wang, K.A. Beauchemin, and T. A. McAllister. 2007. Effects of essential oils and their components on in vitro rumen microbial fermentation. Canadian Journal of Animal Science, 87: 413-419.

9. Benchaar, C., H. V. Petit, R. Berthiaume, T. D. Whyte, and P. Y. Chouinard. 2006. Effect of addition of essential oils and monensin premix on digestion, ruminal fermentation, milk production and milk composition in dairy cows. Journal of Dairy Science, 89: 4352-4364.

10. Bouyeri Kosar, K., H. Shahdadi Jahromy, T. Mohammadabadi, and R. A. Shakeri. 2012. Fermentation parameters and gas production (Myrtle) north and south of Fars Khuzestan in ruminant nutrition. Animal Science Sixth Congress of Iran, Tabriz University.

11. Busquet, M., S. Calsamiglia, A. Ferret, and C. Kamel. 2006. Plant extracts affect in vitro rumen microbial fermentation. Journal of dairy science, 89: 761-771.

12. Busquet, M., S. Calsamiglia, A. Ferret, M. D. Carro, and C. Kamel. 2005. Effect of garlic oil and four of its compounds on rumen microbial fermentation. Journal of Dairy Science, 88: 4393-4404.

13. Çabuk, M., M. Bozkurt, A. Alçiçek., Y. Akbaş, and K. Küçükyılmaz. 2006. Effect of an herbal essential oil mixture on growth and internal organ weight of broilers from young and old breeder flocks. South African Journal of Animal Science, 36: 35-41.

14. Chiofalo, V., L. Liotta, R. Fiumano, E. B. Riolo, and B. Chiofalo. 2011. Influence of dietary supplementation of rosmarinus officinalis L. on performance of dairy ewes organically managed. Journal of small Ruminant Research, 104:122-128.

15. Ghasemi Pirbalouti, A., and A. Aghaee. 2011. Chemical Composition of Essential Oil of Pistacia khinjuk Stocks Grown in Bakhtiari Zagross Mountains, Iran. Electronic Journal Biology, 7(4): 67-69.

16. Ghasemi, A. 2009. Medicinal and Aromatic Plants of Iran (identifying and studying their effects). Azad University sharkord unit. 491 pages.

17. Greathead, H. 2003. Plants and plant extracts for improving animal productivity. Proceedings of the Nutrition Society, 62: 79-290.

18. Hristov, A. N., J. K. Ropp, S. Zaman, and A. Melgar. 2008. Effects of essential oils on in vitro ruminal fermentation and ammonia release. Animal Feed Science and Technology, 144: 55-64.

19. Macheboeuf, D., D. P. Morgavi, Y. Papon, J. L. Mousset, and M. Arturo-Schaan. 2008. Dose response effects of essential oils on in vitro fermentation activity of the rumen microbial population. Animal Feed Science and Technology, 145: 335-350.

20. Mehrabadi, M., A. R. Vakilii, M. Daneshmesgaran, and R. Valizadeh. 2015. The effects of adding different amounts of garlic and eucalyptus on rumen fermentation parameters in vitro. Journal of Modern Agricultural Science and Environment.

21. Mirazadi, Z., B. Pilehvar, M. H. Meshkat Alsadat, and R. Karamian. 2011. Site quality and Essential oil composition of Myrtus Communis L. (case study: Cham moord site in Lorestan province). Journal of Agricultural Biotechnology, 3(2): 71-79.

22. Mozaffarian, V. 2004. Iran trees and shrubs. Publication of Farhang Moaser. The first edition, 964 pages.

23. Noori Norozi, H. 2013. Evaluation of chemical composition, digestibility and fermentation mallow (Mallva sylvesteri) and its effect on rumen fermentation Arabic. Master thesis, Ramin Agriculture and Natural Resources University of Khuzestan.

24. Nourian sarvar, A., and Y. Roozbahan. 2012. Borage extracts on ruminal fermentation, protozoa population and reduces methane production in vitro. Journal of Animal Science, 43) 2(: 287-296.

25. Nourian sarvar, A., and Y. Roozbahan. 2013. Effect of Angelica on rumen fermentation parameters and methane production in vitro. Journal of Animal Science, 44(4): 395-385.

26. Nourian sarvar, A., M. M. Moeni, and F. Vosooghi. 2015. The effect of essential oils Eucalyptus on fermentation parameters and sheep methane production in vitro. Journal of Research in Animal Nutrition, 3: 26-19.

27. NRC. 2007. Nutritional Requirements of Small Ruminant. National Academy Press. Washington, D.C.

28. Nuraliev, I. N. and G. A. Avezov. 1992. The efficacy of quercetin in alloxan diabetes. EKS Perimentalanaia Klinicheskaia Farmakologia, 55: 42-44.

29. Oskoueian, E., A. Norhani, and A. Oskoueian. 2013. Effects of flavonoids on rumen fermentation activity, methane production, and microbial population. Hindawi Publishing Corporation. BioMed Research International. Volume, Article ID 349129, 8 pages. http://dx.doi.org/10.1155/2013/349129.

30. Owlad shanbeh, Y., M. Sari, M. Chaji, T. Mohammadabadi, and M. Bojarpour. 2014. Effects of Native Essence Herb Mrvtlkh (Salvia mirzayanii) on rumen microbial fermentation and nutrient digestibility and gas production system using a continuous two-stream culture. Iranian Journal of Animal Science, 6 (1): 54-65.

31. Patra, A. K. and J. Saxena. 2010. A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Phytochemistry, 71(11-12):1198–1222.

32. Patra, A. K., D. N. Kamra, and N. Agarwal. 2010. Effect of extracts of spices on rumen methanogenesis, enzyme activities and fermentation of feeds in vitro. Journal of Science Food Agriculture, 90:511-520.

33. Payandemehr, M., M. Boujarpour, M. Sari, T. Mohammadabadi, and M. Chaji. 2014. Investigating the effects of Eucalyptus essential oils on fermentation parameters in high concentrate diets using gas production technique. Sixth Congress of Animal Sciences, University of Tabriz, Iran.

34. Popovic, M., B. Kaurinovic, S. Trivic, N. Mimica-Dukic, and M. Bursac. 2006. Effect of celery (Apium graveolens) extracts on some biochemical parameters of oxidative stress in mice treated with carbon tetrachloride. Phytochemical Research, 20: 531-537.

35. Rezaei, F. 2014. Investigating the effect of Scrophularia striata Boiss on fermentative and rumen microbial digestion of Lorry- BAKHTEYARI sheep. Thesis of Masters Science Animal nutrition. Ramin Agriculture and Natural Resources University of Khuzestan.

36. Rooghani, M., D. Baluchneghad, T. Mojarad, and M. Ramazani. 2007. The effect of chronic oral administration of the aviation sector Ombo, much of global glucose and lipids Great White streptozotocin-induced diabetes in mice. Iranian Journal of Medicinal and Aromatic Plants Research, 33 (4): 467-458.

37. Rostamzadeh, H., R. Pirmohammadi, and Y. A. Alijoo. 2015. Effect of Ajowan plant essential oil (cariuincopticun) on performance and some blood parameters of Mahabadi goats at early lactation period. Animal Sciences Journal, 106: 103-110.

38. Saeedi, S., and A. Diani. 2014. The effect of feeding different levels of fennel powder in starter diets on performance of Holstein dairy calves. Sixth Congress of Animal Science, University of Tabriz.

39. Safari, H., M. Mohiti, and M. Dejamkhoei. 2014. The effect of different levels of dried powder of purslane on blood metabolites of fattening lambs. 6the Animal Sciences Congress, University of Tabriz. (In Farsi).

40. Sahraei Belverdy, M., and R. Pirmohammadi. 2014. Effects of Rosemary (Rosmarinus officinalis L.) essential oils on digestibility, blood parameters of Ghezel Sheep. Animal Sciences Journal, 103: 71-82.

41. Smith, H., E. Zoetendal, and R. I. Mackie. 2005. Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microbial Ecology, 50 (2):197-205.

42. Theodorou, M. K., B. A. Williams, M. S. Dhanoa, A. B. McAllan, and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 74: 3583-3597.

43. Vakili, A. R., B. Khorrami, M. Danesh Mesgaran, and E. Parand. 2013. The effects of thyme and cinnamon essential oils on performance, rumen fermentation and blood metabolites in Holstein calves consuming high concentrate diet. Asian-Australasian Journal of Animal Science, 26: 935-944.

44. Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods of dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74: 3583- 3597.