Effects of Ca, Mg and GA3 Spray on Bunch Stem Necrosis Disorder in Grape (Vitisvinifera cv. Bidaneh Sefid)

علوم باغبانی

Article 5, Volume 32, Issue 1, July 2018, Pages 109-122   

Document Type: Research Article

DOI: 10.22067/jhorts4.v32i1.61849

Authors

Hamed Doulati Baneh^{* 1}; marzieh Mohammadzade²; Farokh Ghani Shayeste¹

¹Research and Education center of west Azarbaijan Agricultural and Natural Resources Research Center

²University of Urmia

Abstract

IntroductionLate-season Bunch Stem Necrosis (BSN) is observed as a necrosis of the cluster stem (rachis) that leads to shriveling of berries on the affected portion of the cluster. The BSN symptoms include dark, necrotic lesions on the rachis or individual pedicels that may spread and eventually girdle the affected part of the cluster rachis. Berries distal to a lesion cease normal development, and the unripe berries either abscise or remain on the cluster in a withered condition. Frequently only the cluster tip or a shoulder is affected, while the rest of the cluster develops normally. Symptomatic and non-symptomatic clusters may be borne on the same vine. BSN has been correlated with numerous factors; however, no universal cause and effect relationships have been demonstrated. No pathogens are believed to cause this condition. Instead, certain weather conditions and vine nutrition seem to be associated with its occurrence. Low temperatures and high humidity around bloom or excessive rainfall after veraison may be related to its development. Imbalances between calcium and potassium as well as low levels of nitrogen in vines are other possible causes. Unaffected portions of clusters develop normal fruit quality.ʽBidaneSefidʼ or ʽKeshmeshiʼ is an important grape cultivar in Iran and is frequently affected by bunch stem necrosis disorder around country. The purpose of this study was to determine if mineral nutrition was associated with BSN of ʽBidaneSefidʼ grape cultivar under Urmia growing conditions.
Materials and MethodsTo evaluate the effects of foliar sprays of Ca, Mg and GA3 on reduction of BSN incidence on ʽBidaneSefidʼ cultivar, and to examine the relationships between specific nutrients and the incidence of BSN this field experiment was performed based on randomized complete blocks design with eight treatments and three replicates on 2011in a vineyard around Urmia city. Vines were 8-year-old and were trained as bi lateral cordon. At the end of full bloom and a week after that the vines were sprayed two and five times with 2% MgSO4 and CaCl2 fertilizers alone and combined. GA3 also was sprayed at 70% of flowering with 20 ppm and replicated at fruit set with 40 ppm. Symptoms rate of BSN on bunches and minerals nutrient content(N, K, Ca, Mg and Ca+Mg/K) of leaves and rachis were measuredin veraison phenological stage.
Results and DiscussionResults showed that in veraison stage the most and the least BSN incidence rate was recorded in control and the vines that were sprayed two times with 2% MgSO4, respectively. Leaf Ca content in vines treated two times with 2% CaCl2 and those sprayed five times with MgSO4+CaCl2 was more than other treatments. Maximum amount of Mg was recorded in leaves of vines sprayed by 2% MgSO4, 5 times during growing season. These vines showed the least BSN rate, too. The most contentof K/Ca+Mg were in the leaves of control and GA3 treated vines and the least contentof those was in vines treated five times with MgSO4+CaCl2. Rachis tissue analysis for nutrient elements in veraisonstage did not consistently reveal any relationship between N, K, Mg, Ca and Mg+Ca/k and bunch stem necrosis in bunches of studied vines of ʽBidaneSefidʼ cultivar in Urmia region. There are conflicting reports regarding the association of essential nutrients and the incidence of BSN. A high ratio of potassium to magnesium and/or calcium in affected tissues, and also the application of calcium and/or magnesium fertilizers effectively reduced the incidence of BSN in Europe. In California, BSN was not reduced by applications of calcium and magnesium. An increase in the incidence of BSN was reported with application of nitrogen fertilizers. Magnesium and calcium appeared to be involved in the disorder. The results illustrate that BSN-prone vineyards should be individually examined for nutrient imbalance or other stresses that may be contributing to BSN.
ConclusionsResults of this study suggest thecontrol of BSN incidence by fertilizer treatments. Foliar applications ofMg and Ca fertilizers appeared to have effect on BSN control and two times foliar applications of magnesium starting just before veraison minimize the problem. No apparent relationship was found between the petiole and/or rachis K/(Mg + Ca) ratio and BSN incidence. External application of GA3 reduced the BSN incidence in comparison to control.

Keywords

Abscission; Berry; disorder; Grapevine; Nutrition

References

1- Baldacchino-Reynaud C. 2000. Stalk necrosis of Muscat de Hambourg, Arboriculture Fruitiere, 540: 19-23.

2- Boselli M., Scienza A., Dorotea G. and Volpe B. 1995. Possibilities of avoiding stalk necrosis by controlling mineral nutrition, Vignevini, 10:35–38.

3- Brendel G., Stellwaag-Kittler F. and Theiler R. 1983. Pathological-physiological criteria of stalk necrosis, Mitteilungen Klosterneuburg, 33:100–104.

4- Capps E.R. and Wolf T.K. 2000. Reductions of bunch stem necrosis of Cabernet Sauvignon by increased tissue nitrogen concentration, American Journal of Enology and Viticulture, 51:319–328.

5- Christensen L.P. and Boggero J.D. 1985. A study of mineral nutrition relationships of waterberry in Thompson Seedless, American Journal of Enology and Viticulture, 36:57–64.

6- Cooper T, Castro D. and Retamales J. 1987. Factors influencing stalk necrosis in the table grape Sultanine, in Chile, Chemical control of this disorder, Progres Agricole Viticole, 104:467–471.

7- Dilmaghani Hasanlooii M.R., Hemati S., Doulati Baneh H., Agheli Mghanjouyi V. and Nikkhahi Dastjerdi Y. 2014. Effects of different potassium and magnesium ratios on cluster necrosis and fruit quality in Sefid Bidaneh grape, Iranian Journal of Horticultural Science, 45: 207-216.

8- Emami A. 1997. Plant Analysis Methods. Agricultural Research, Education and Extension Organization Publication, Iran. 128 pp (in Persian).

9- Gluhic D., Custic M.H., Petek M., Coga L., Slunjski S. and Sincic M. 2009. The Content of Mg, K and Ca Ions in Vine Leaf under Foliar Application of Magnesium on Calcareous Soils, Agriculturae Conspectus Scientificus, 74:81-84.

10- Hartmair V. and Grill F. 1965. Ein beitrag zum problem der Stiella¨hme der weintrauben, Mitteilungen Klosterneuburg, 15:10–12.

11- Haub G. 1983. Experiments on stalk necrosis control; results and experiences, Mitteilungen Klosterneuburg, 33:142–146.

12- Holzapfel B.P. and Coombe B.G. 1995. Incidence of grapevine bunch stem necrosis in South Australia: effects of region, year and pruning, Australian Journal of Grape Wine Research, 1:51–54.

13- Jordan D.T. 1989. Ammoniumin grapevines: Seasonal levels in tissue and xylem extracts, and tendril model system. Ph.D. thesis, Oregon State University, Corvallis.

14- Koblet W., Candolfi-Vasconcelos M.C. and Keller M. 1997. How do grapevines respond to altered source/sink ratios and unfavorable environmental conditions? Proceedings of the 4th international symposium on cool climate viticulture and enology, Rochester, NY 16–20 July 1996. Communications Services, New York.

15- Krasnow M., Weis N., Smith R., Benz J., Matthews M. and Shackel K. 2009. Inception, Progression, and Compositional Consequences of a Berry Shrivel Disorder, American Journal of Enology and Viticulture, 60:24-34.

16- Morrison J.C., and Iodi M. 1990. The influence of waterberry on the development and composition of Thompson Seedless grapes, American Journal of Enology and Viticulture, 41:301–305.

17- Pickering A.H. 2006. Factors affecting the predisposition of ‘Cabernet Sauvignon’ grapevines (Vitis vinifera L.) to the physiological disorder, bunch stems necrosis. Ph.D. thesis, Massey University, Palmerston North, NZ.

18- Pickering A.H., Warrington I.J., Woolley D.J., and Wunsche J.N. 2004. A reduction in vine vigour of Cabernet Sauvignon (Vitis vinifera L.) decreases bunch stem necrosis, Acta Horticulturae, 732:687–692.

19- Redl H. 1983. Development of macro- and micronutrient contents in grape stalk, berries and leaves during ripening in relation to stalk necrosis, Mitteilungen Klosterneuburg, 33:39–59.

20- Redl H. 1987. Investigations concerning the prognosis of stem necrosis under Austrian wine growing conditions, Mitteilungen Klosterneuburg, 37:109–113.

21- Ruiz S.R. and Moyano A.S. 1994. The problem of stalk necrosis in grapevines and its relationship with elevated putrescine levels and low potassium content, Agricultura Tecnica, 54:87–94.

22- Schaller K. 1977. Relationships between different nutrient fractions in the soil and the incidence of stalk withering in vines, Mitteilungen Klosterneuburg, 27:151–155.

23- Klaus Schaller, K. and Lehnhart, R. 2016. Chemical Bonds of Cations in Grapevine Berries and Stems of Some Important Cultivars of Vitis vinifera (L.) and Their Relevance for Viticulture and Enology, Bulletin UASVM Horticulture, 73(2): 196-204.

24- Scienza A.1982. Recent information on the causes of stalk withering, Vignevini, 9:15–30.

25- Scienza A. and Fregoni M. 1978. The relationship between the number of berries in the cluster and grapevine rachis desiccation, Vignevini, 5:31–37.

26- Shayesteh F. and Doulati Baneh H. 2002. Causes of berry and flowers abscission, cluster shrivel of vines in gardens around urmia. (Annual Report1387:219). Soil and Water Research Institute, Urmia, Iran (In Persian).

27- Shin K.C., Moon J.Y., Choi J.S. and Kim S.B. 1984. Studies on the causes of grape stalk necrosis, Research Reports, Office of Rural Development, South Korean Horticulture Research Report, 26:10–14.

28- Stellwaag-Kittler F. 1975. Studies to elucidate the causal mechanism of stalk withering in grapes. Mitteilungen Klosterneuburg, 25:3–18.