Effect of Different Diets of Flour Moth on its Parasitoid Wasp Fitness, Trichogramma brassicae (Hym.:Trichogrammatidae)

پژوهش های حفاظت گیاهان ایران

Article 2, Volume 30, Issue 4 - Serial Number 34, December 2017, Pages 709-717   

Document Type: Research Article

DOI: 10.22067/jpp.v30i4.53205

Authors

Paria Soltaninejad¹; Asghar Shirvani^{* 1}; Maryam Rashki²

¹Shahid Bahonar University of Kerman

²Graduate University of Advanced Technology, Kerman

Abstract

Introduction: Parasitoid wasp, Trichogramma brassicae (Bezdenko) (Hym.: Trichogrammatidae) has excellent position in mass production technology, scope of application, wide geographical distribution and unmatched talent to adapt to different climatic conditions that can be easily reared on Mediterranean flour moth (MFM), Ephestia kuehniella Zeller (Lep.: Pyralidae). The parasitoid use is in order to control Chilo suppressalis (Walker) (Lep.: Pyralidae), Helicoverpa armigera (Lep.: Noctuidae), Ostrinia nubilalis (Hübner) (Lep.: Pyralidae), Ectomyelois ceratoniae (Zeller) (Lep.: Phycitidae) and Cydia pomonella (Lep.: Tortricidae) (38). The studies have been shown that the quantity and quality of the host diet affect growth period, size of adults, adult longevity, fecundity and sex ratio of the parasitoid. The objective of current research was to evaluate the effect of four different MFM diets on the fitness of second and forth generations of the parasitoid wasp, T. brassicae.
Materials and Methods: The four diets ((I) wheat flour, wheat bran, corn flour, bread yeast and glycerin; (II) wheat flour, wheat bran, barley flour, bread yeast and glycerin; (III) barley flour, bread yeast and glycerin and (IV) wheat flour, barley flour and corn flour) were sterilized at 51 °C for 24 hours. After cooling at ambient temperature, the diets were contaminated with the eggs of MFM and then were maintained at 25±1° C, 60±5 % RH and a photoperiod of 14 L: 10 D. The MFM adults emerged after 35 to 40 days and their eggs were collected daily to use for investigation of the parasitoid biology. For rearing the parasitoid wasps on the MFM eggs obtained from each diet, some cards containing the parasitoid pre-pupae were put inside cages (25 × 25 × 25 cm) and reared for one generation. The adults were fed honey (20%) for one day and then were provided with one-day-old sterile MFM eggs. To prevent egg hatching and sterile them, they were kept at 15°C for four h. The tests were conducted with 10 replicates for each treatment at 25±1°C, 60±5 % RH and a photoperiod of 14 L: 10 D. Each glass tube (8cm diameter, 11 cm height) as an experimental unit was consisted of 40 and 20 wasps for the second and fourth generation of the parasitoid and was provided with the card carried 200 and 100 eggs of MFM, respectively. The number of emerged parasitoid wasps along with the number of parasitized eggs on each card w recorded daily till the death of the adult wasp. Also, the number of emerged wasps and females were counted.
Results and Discussion: The results showed that the lowest parasitism rate was occurred in the treatment I (9.8±0.011 %) for the second generation of the parasitoid. But, in the fourth generation, the treatment II and III presented the highest (11.05±1.23 %) and lowest parasitism rate (5.58±0.70%), respectively. In the previous report, the parasitism rate of T. brassicae fed on eggs of Sitotroga cerealella was assessed higher than that in the present study, which occurs probably because of the differences in insect host.
The results obtained from the second generation showed that the variation in the diets had significant effect on the percentage of parasitoid emergence and the highest percentage of the parasitoid emergence was observed in treatment I (64.5±4.78 %) but there was no significant difference between the treatment I and III. The percentage of adult emergence of Trichogramma pretiosum Riley (28) and Trichogramma maidis Pint, et Voeg. (13) reared on the MFM eggs were higher than the present research. The difference may be related to the parasitoid density and the wasp species. However, the variation in diets had no significant effect on the percentage of parasitoid emergence in fourth generation and sex ratio in both generations. In second generation, there were no significant differences among the treatments in daily oviposition of the female parasitoid but in fourth generation, various diets had significant effect. The highest and lowest daily oviposition were found for treatment II (0.77±0.07) and IV (0.42±0.06), respectively. The treatment III and II meaningfully caused the longest parasitoid lifetime in the second (12.31 ± 0.09 days) and forth generation (12.36±0.03 days), respectively. Nevertheless, no significant variation in lifetime of the parasitoid between the other treatments was found in the second and forth generations, separately. Several biotic and abiotic factors can influence T. brassicae lifetime such as temperature, adult feeding, humidity, the quality of the host egg and the size of parasitoids. Also, the proportion of wasps surviving at time x (lx) were almost similar among all treatments in the second and forth generations, separately. The survival rate fluctuations of T. brassicae reared on eggs of Ch. suppressalis, were similar on different rice varieties and after eight days all adults were dead which was similar to our results.
Conclusion: By referring to the results, treatment II, included wheat flour, wheat bran, barley flour, bread yeast and glycerin (ratios: 41.5: 2: 43.5: 3: 10%, respectively) is proposed to optimize mass rearing of the parasitoid wasp, T. brassicae as an important biological control agent. The treatment II caused to increase the life span and parasitism rate of T. brassicae and is suitable for mass production of the parasitoid to promote the quality of biological control programs.

Keywords

Biological control; Life span; Parasitism; Wheat flour

References

1- Abroun P., Mousavi S.Gh., Ashouri A., and Gishani H. 2013. Effect of different quality of Ephestia kuehniella on the parasitism of Trichogramma brassicae. p. 1-8. The 1st National Conference on Stable Agriculture and Natural Resources. (in Persian).

2- Ahmadpour S., Iranipour Sh., and Asgari Sh. 2013. Effects of superparasitism on reproductive fitness of Ooencyrtus fecundus (Hym.: Encyrtidae), egg parasitoid of Sunn pest, Eurygaster integriceps (Hem.: Scutelleridae). p. 2. Conference of Biological Control in Agriculture and Natural Resources, University of Tehran, 27-28 August. 2013. (in Persian with English abstract).

3- Akbarzadeh Shoukat G. 2006. Study of efficacy of walnut fruit worm egg parasitoid wasp Trichogramma embryophagum in laboratory condition. p. 20. In Manzari, S. (Ed.) p. 418. Proceedings of the 17th Iranian Plant Protection Congress, Vol. I, Pests. (in Persian).

4- Attaran M.R., Shojaii M., and Ebrahimi E. 2000. Effects of hosts and feeding on the longevity and the number of eggs deposited by Trichogramma brassicae. p. 173. Proceedings of the 14th Iranian Plant Protection Congress. I, Pests.

5- Babendreier D., Kuske S., and Bigler F. 2002. Overwintering of the egg parasitioid Trichogramma brassicae (Hym.: Trichogrammatide) in Northern Switzerland. Biological Control, 52:37-45.

6- Bidar F., Naseri B., Razmjo J., and Fathi S.A.A. 2013. Performance Nutrition of the Mediterranean flour moth, Ephestia kuehniella Zeller On the different varieties of barley. p. 37-39. Second national congress and conventional organic agriculture. (in Persian).

7- Bonte M., and DeClercq P. 2008. Developmental and reproductive fitness of Orius laevigatus (Hemiptera: Anthocoridae) reared on factitious and artificial diets. Journal of Economic Entomology, 101 (4):1127-1133.

8- Bigler F. 1994. Quality control in Trichogramma production. Biological control with egg parasitoid. p. 93-111. CAB International, UK.

9- Cerutti F., and Bigler F. 1995. Quality assessment of Trichogramma brassicae in the laboratory. Entomologia Experimentalis et Applicata, 75:19-26.

10- Chirane J., and Lauge G. 1996. Loss of parasitization efficiency of Trichogramma brassicae (Hym.: Trichogrammatidae) under high temperature conditions. Biological Control, 7:95-99.

11- Ebrahimi A. 2004. Study and distinguishing the species in the genus Trichogramma Westwood in Iran. Debates about use of natural enemies for biological control of pests. p. 1-71. Press Publishing Centre Sepehr, (in Persian).

12- Etzel L.K., and Legner E.F. 1999. Culture and colonization. In: Bellows, T.S., Fisher, T.W. (eds). Handbook of biological control. p. 125-198. Academic Press.

13- Farazmand A., Irani pur Sh., Saber M., and Mashhadi Jafarlu M. 2007. Comparison of some biological parameters of Trichogramma brassicae Bez biological control agent against the Indian meal moth Plodia interpunctella (Lep.: Pyralidae) and flour moth Ephestia kuehniella (Lep.: Pyralidae). Agricultural Science, 17 (3):175-187. (in Persian).

14- Garcia P., and Tavares J. 1994. Parasitic capacity, longevity and development of Trichogramma maidis (Hym.: Trichogrammatidae) at three temperature regimes. Trichogramma and Other Egg Parasitoids, 4:71-74.

15- Garcia P.V., Wajnberg E., Oliveira M.L.M., and Tavares J. 2001. Is the parasitization capacity of Trichogramma cordubensis influenced by the age of the females?. Entomologia experimentalis et applicata, 98:219-224.

16- Gomez L.L.A., Diaz A.E., and Lastra L.A. 1995. Selection of strains of Trichogramma exiguum for controlling sugarcane borers (Diatraea spp.) in the Cauca valley, Colombia. Less Colloques de l’ INRA, 73:75-78.

17- Haile A.T., Hassan S.A., Sithanantham S., and Ogol C. 2002. Comparative life table analysis of Trichogramma bournieri Pintureau and Babault and Trichogramma sp. nr. mwanzai Schulten and Feijen (Hym.: Trichogrammatidae) from Kenya. Journal of Applied Entomology, 126:287-292.

18- Haghani M., and Fathipour Y. 2003. The effect of the type of laboratory host on the population growth parameters of Trichogramma embryophagum Hartig (Hym, Trichogrammatidae). Journal of Agricultural Sciences and Natural Resources, 10(2):117-123. (in Persian with English abstract).

19- Hassan S.A., and Zhang W.Q. 2001. Variability in quality of Trichogramma brassicae (Hym.: Trichogrammatidae) from commercial suppliers in Germany. Biological Control, 22:115-121.

20- Hegazi E.M., Khafagi W.E., and Hassan S.A. 2000. Studies on three species of Trichogramma: foraging behavior for food or hosts. Journal of Applied Entomology, 124:145-149.

21- Hohmann C.L., Luck R.F., Oatman E.R., and Platner G.R. 1989. Effects of different biological factors on longevity and fecundity of Trichogramma platneri Nagarkatti (Hymenoptera; Trichogrammatidae). Annals of the Entomological Society of Brasil, 18:61- 70.

22- Karimian Z., and Sahragard A. 2000. Investigation on biology of Trichogramma brassicae the egg parasitoid of major pest of rice in Guilan province. p. 22. Proceedings of the 14th Iranian Plant Protection Congress, Vol. I, Pests.

23- Klemm U., and Schmutterer H. 1992. Selection of effective egg parasitoids of the genera Trichogramma and Trichogrammatoidea (Hym.: Trichogrammatidae) and diamondback moth control in Taiwan. Mitteilungen der Deutschen Gesellschaft fur Allgemeine und Angewandte Entomologie, 8:82-86.

24- Knutson A. 1998. The Trichogramma Manual, Texas Agricultural Extension Service.

25- Kuhlmann U., and Mills N.J. 1999. Comparative analysis of the reproductive attributes of three commercially produced Trichogramma spp. (Hym.: Trichogrammatidae). Biocontrol Science and Technology, 9: 335-346.

26- Karimi Malati A., and Hatami B. 2010. Effect of feeding and male presence on some biological characteristics of female Trichogramma brassicae (Hymenoptera: Trichogrammatidae). Jornal of entomological society of Iran, 29(2):1-11.

27- Leatmia J.A., Laing J.E., and Corrigan J.E. 1995. Production of exclusively male progeny by mated honey-fed Trichogramma minutum (Hym.: Trichogrammatidae). Journal of Applied Entonology, 119:561-566.

28- Lundgren G.E., and Bomgren S.A. 2002. Comparison of Trichogramma brassicae (Hym.: Trichogrammatidae) augmentation with organic and synthetic pesticides for control of cruciferous Lepidoptera. Biological Control, 31:1231-1239.

29- Lundgren J.G., and Heimpel G.L. 2002. Quality assessment of three species of commercially produced Trichogramma and the first report of thelytoky in commercially produced Trichogramma. Biological control, 26:68-73.

30- Nogueira De Sa L.A., and Parra J.R.P. 1994. Biology and parasitism of Trichograrnma pretiosum Riley (Hym., Trichogrammatidae) on Ephestia kuehniella (Zeller) (Lep., Pyralidae) and Heliothis zea (Boddie) (Lep., Noctuidae) eggs. Journal of applied entomology, 118:38-43.

31- Ozkan C. 2006. Effect of host diet on the development of the solitary egg-larval parasitoid Chelonus oculator in superparasitized Ephestia kuehniella. Phytoparasitica, 34(4):338-346.

32- Pompaon F., Fouillet P., and Bouler M. 1995. Emergence rhythms and prothandry in relation to a daily pattern of locomotor activity in Trichogramma species. The Canadian Entomologist, 25:139-146.

33- Ranjbar Aghdam H., and Mahmoudian R. 2014. Effect of Different Rice Varieties on Age Specific Life Table and Population Growth Parameters of Trichogramma brassicae, the egg parasitoid of the striped stem borer, Chilo suppressalis. Iranian Journal of Plant Protection Science, 45(1): 1-11. (in Persian with English abstract).

34- Razmjou J., Moharramipour S., Fathipour Y., and Mirhoseini S.Z. 2006. Effect of cotton cultivar on performance of Aphis gossypii (Homoptera: Aphididae) in Iran. Journal of Economic Entomology, 99:1820-1825.

35- Safa M., Yazdanian M., and Sarailoo M.H. 2014. Larval feeding from some artificial diets and its effects on biological parameters of the mediterranean flour moth. Munis Entomology and Zoology, 9(2):678-686.

36- Sedlacek J.D., Weston P.A., and Barney R.J. 1996. Lepidoptera and psocoptera. In: Subramanyam B, Hagstrum DW, editors. Integrated management of insects in stored products. New York (NY): Marcel Dekker; pp. 41-70.

37- Smith S.M., and Hubbes M. 1986. Isoenzyme patterns and biology of Trichogramma minutum as influenced by rearing temperature and host. Entomologia Experimentalis et Applicata, 42:249-258.

38- Soltani Nezhad P., Shirvani A., and Rashki M. 2014. Effect of different diets on rearing flour moth, Ephestia kuehniella Zeller. p. 875-880. First Iran's pistachio conference, 31 August-1 September 2014. (In Persian).

39- Suh C.P.C., Orr D.B., and Van Duyn J.W. 2000. Trichogramma releases in North Carolina cotton: Why releases fail to suppress Heliothine pests. Journal of Economic Entomology, 93:1137-1145.

40- Tarlak P., Mehrkhou F., and Mousavi M. 2014. Life history and fecundity rate of Ephestia kuehniella (Lepidoptera: Pyralidae) on different wheat flour varieties. Archives of phytopathology and protection. p. 25-34.

41- Tillman P.G., and Cate J.R. 1993. Effect of host size on adult size and sex ratio of Bracon melitor (Hymenoptera: Braconidae). Environmental Entomology, 22:1161-1165.

42- Uzun S. 1994. Studies on the parasitoid host relationship of Trichogramma brassicae Bez. (Hym.: Trichogrammatidae) and eggs of the Mediterranean flour moth Ephestia kuehniella Zell. under different temperatures and storage periods. Journal of Applied Entomology, 119:431- 440.

43- Vaez N., Nouri Gh., Ganbalani Iranipour Sh., Mashhadi Jafarloo M., and Asghari Zakaria R. 2009. Necessity of encountering Trichogramma brassicae Bezdenko wasps reared on alternative hosts, cereal moth Sitotroga cerealella Hubner and flour moth Anagasta kuehniella Zeller to target pest bollworm Helicoverpa armigera Hubner prior to release. Journal of Agricultural Knowledge, 19(1):317-332. (in Persian).

44- Weiru Z., and Ren W. 1989. Rearing of Orius sauteri (Hemiptera: Anthocoridae) with natural and artificial diets. Chinese journal of biological control, 5:9-12.

45- Xu J., Wang Q., and He X.Z. 2007. Influence of larval density on biological fitness of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). New Zealand Plant Protection, 60:199-202.

46- Yazdani Khorasgani A., Hosseini Bai Sh., Hadad Irani Nezhad K., and Mashhadi Jafarloo M. 2006. An investigation on some biological traits of Trichogramma brassicae Bezd. on the eggs of angoumois grain moth Sitotroga cerealella Oliv. Journal of agricultural sciences, 19(1): 271-282. (in Persian with English abstract).

47- Yu D.S.K., Hagley E.A., and Laing J.E. 1984. Biology of Trichogramma minutum Riley. Collected from apples in Southern Ontoria. Environmental Entomology, 13:1324-1329.