Gas Production and In vitro Digestibility of Various Sources of Non-fiber Carbohydrates and Rumen Degradable Protein

پژوهشهای علوم دامی ایران

Article 4, Volume 11, Issue 4 - Serial Number 40, December 2020, Pages 413-424   

Document Type: Ruminant Nutrition

DOI: 10.22067/ijasr.v11i4.69793

Authors

Eshagh Esmaili doabi¹; Javad Bayat Koohsar¹; Farkhondeh Rezaii^{* 2}; Yousef Mostafalou¹; Ashoor Mohammad Gharebash¹

¹Department of Animal Science, Faculty of Agriculture, Gonbad Kavos University, Gonbad Kavos, Iran

²Agriculture Department, Payame Noor University, Shahrood, Iran

Abstract

Introduction :Carbohydrates are generally classified as either non-structural or structural carbohydrates. Structural carbohydrates include cellulose, hemicellulose and lignin, and non-fiber carbohydrates (NFC) are present inside the plant cells and are usually more digestible than carbohydrates found in plant cell walls. Dietary non-fiber carbohydrates are the main source of energy for high-producing dairy cows, which provide 30 to 45 percent of the dry matter intake. They are a very diverse group of non-fiber carbohydrates, which include starch, sugars, fructans, and pectin substances. Although the total amount of non-fiber carbohydrates is referred to as a single value, different types of NFC have different effects on digestibility and production properties. The dietary profile of NFC has the potential to alter the supply of metabolizable nutrients to the animal because NFC differ in digestion and fermentation characteristics. Sucrose is rapidly fermented in the rumen compared with corn starch. In vitro fermentation of sucrose, starch, and pectin resulted in different organic acid profiles, digestion characteristics, and maximal microbial protein yields. In addition, these effects may be affected by other feed components, such as the amount of rumen degradable protein. Some researchers have been showed that the impact of starch and sugars on low quality forage utilization may interact with the amount of supplemental rumen degradable protein.
This experiment was conducted to investigate the effect of various sources of non-fiber carbohydrates (starch, sucrose, pectin, dextrose) in combination with different levels of rumen degradable protein on gas production and digestibility of fermentation parameters in in vitro condition.
Materials and Methods: Soybean meal treated with heating at 160°C-60 min and untreated soybean meal were selected as low rumen degradable protein (RDP) and high RDP sources, respectively. Four types of non-fiber carbohydrates (dextrose, sucrose, starch and pectin) supplemented with two levels of rumen degradable protein (RDP) in a 2×4 factorial arrangement by gas production and batch culture. A basal diet including: alfalfa (30%), corn silage (10%), barley grain (25%), wheat barn (9%), salt (0.5%), mineral-vitamin supplement (0.5%), soybean meal (15%) and non- fiber carbohydrate source (10%) was prepared. This study was conducted in two experiments. In the first experiment, the gas production parameters for two levels of high and low rumen degradable protein and four types of non-fiber carbohydrates (starch, sucrose, pectin and glucose) using the gas production technique. In the second experiment, the digestibility of the first experimental treatments were measured in an in vitro batch culture method. In the first experiment, the gas pressure was recorded with the pressure gauge at 0, 2, 4, 6, 8, 12, 24, 36, 48, 72, 96 h after incubation. In the second experiment treatments were incubated in batch culture conditions for 24 hours and the degradability parameters were determined using equations.
Results and Discussion: the obtained results showed that sucrose had highest gas production potential, organic matter digestibility (OMD), metabolizable energy (ME) and short chain fatty acids
 (SCFAs) than other NFC type in low RDP level. There was a significant difference in digestibility of organic matter and dry matter between treatments (P<0.05). The highest and lowest ammonia nitrogen concentrations were obtained from treatments with starch in combination with high and low RDP, respectively. Treatments had a significant effect on partitioning factor and production of microbial mass (P<0.05). Among the low-degradability protein-based treatments, pectin treatment had the highest amount of microbial production and among the treatments with high degradability protein content, starch treatment had the highest production of microbial mass and partitioning factor. Overall, the results showed that when the RDP level is low, sucrose has a better performance in terms of fermentation parameters, whereas when using the high RDP, there was no significant difference between different types of carbohydrates. It seems that these interactions should be considered in regulating diets.
Conclusion: The results of this experiment showed that different types of non-fiber carbohydrates and levels of rumen degradable protein had a different responses to fermentation parameters. When using low protein levels, it seems that there is a greater difference between different sources of non-fiber carbohydrates, while at high levels of protein degradation there is no significant difference between the different sources of non-fiber carbohydrates in terms of digestibility. Based on these results, it can be concluded that the non-fiber carbohydrate sources, along with the degradable protein supplement, has the ability to change the characteristics of ruminal fermentation, the source of food and the production.
 

Keywords

Degradable protein; Gas production; Non-fiber carbohydrates; digestibility

References

Amanzougarene, Z., S. Yuste, A. Vega, and M. Fondevila. 2017. In vitro fermentation pattern and acidifiation potential of different sources of carbohydrates for ruminants given high concentrate diets. Spanish Journal of Agricultural Research, 15(2): 2171-9292.

2- AOAC. 2005. Official Methods Of Analysis. 18th ed. Vol. 1. No. 1. Association of Official Analytical Chemists Washing Town, D.C.

3- Barrios-Urdaneta, A., M. Fondevila, and C. Castrillo. 2003. Effect of supplementation with different proportions of barley grain or citrus pulp on the digestive utilization of ammonia-treated straw by sheep. Journal of Animal Science, 76: 309-317.

4- Blummel, M., H. P. S. Makkar, and K. Becker. 1997. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition, 77: 24-34.

5- Broderick, G. A. and J. H. Kang. 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, 63: 64–75.

6- Broderick, G. A. and W. J. Radloff. 2002. Effects of replacing dietary high moisture corn with dried molasses on production of dairy cows. U.S. Dairy Forage Research Center 2000-2001. Research Report, pp. 106-109.

7- Chamberlain, D. G., S. Robertson, and J. J. Choung. 1993. Sugars versus starch as supplements to grass silage: Effects on ruminal fermentation and the supply of microbial protein to the small intestine, estimated from the urinary excretion of purine derivatives, in sheep. Journal of Science of Food and Agriculture, 63: 189–194.

8- Chester-Jones, H., M. D. Stern, H. M. Metwally, J. G. Linn, and D. M. Ziegler. 1991. Effects of dietary protein-energy interrelationships on Holstein steer performance and ruminal bacterial fermentation in continuous culture. Journal of Animal Science, 69: 4956- 4966.

9- Hall, M. B. 2002. Working with sugars (and molasses). In Proceedings from the 13th Annual Florida Ruminant Nutrition Symposium, Gainesville, FL.

10- Hall, M. B. and C. Herejk, 2001. Differences in yields of microbial crude protein from in vitro fermentation of carbohydrates. Journal of Dairy Science, 84: 2486–2493.

11- Hall. M. B., C. C. Larson, and C. J. Wilcox. 2010. Carbohydrate source and protein degradability alter lactation, ruminal, and blood measures. Journal of Dairy Science, 93: 311–322.

Heldt, J. S., R. C. Cochran, G. L. Stokka, C. G. Farmer, C. P. Mathis, E. C. Titgemeyer, and T. G. Nagaraja. 1999. Effects of different supplemental sugars and starch fed in combination with degradable intake protein on low-quality forage use by beef steers. Journal of Animal Science, 77: 2793–2802.

12- Huhtanen, P. 1988. The effects of barley, unmolassed sugar-beet pulp and molasses supplements on organic matter, nitrogen and fibre digestion in the rumen of cattle given a silage diet. Journal of Animal Feed Science and Technology, 20 (4): 259-278.

13- Kim, K. H., J. Choung, and D. G. Chamberlain. 1999. Effects of varying the degree of synchrony of energy and nitrogen release in the rumen on the synthesis of microbial protein in lactating dairy cows consuming a diet of grass silage and a cereal-based concentrate. Journal of Science of Food and Agriculture, 79: 1441–1447.

14- Lykos, T., G. A. Varga, and D.Casper. 1997. Varying degradation rates of total nonstructural carbohydrates: Effects on ruminal fermentation, blood metabolites, and milk production and composition in high producing Holstein cows. Journal of Dairy Science, 80: 3341– 3355.

15- Makkar, H. P. S. 2004. Recent advances in the in vitro gas method for evaluation of nutritional quality of feed resources. Assessing Quality and Safety of Animal Feeds. FAO Animal Production and Health Series 160. FAO, Rome, pp. 55–88.

16- Makkar, H. P. S. 2005. In vitro gas methods for evaluation of feeds containing phytochemicals. Animal Feed Science and Technology, 124: 291-303.

17- Menke, K. H., L. Raab, A. Salewski, H. Steingass, D. Fritz, and W. Schneider. 1979. The estimation of the digestibility and metabolisable energy content of ruminant feeding stuffs from the gas production when they are incubated with rumen liquor in vitro. Journal of Agriculture and Food Science, 93: 217– 222.

18- Menke, K. H. and H. Steingass. 1978. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Science Development, 28: 7-12.

19- Nocek, J. E. and J. B. Russell. 1988. Protein and energy as an integrated system: Relationship of ruminal protein and carbohydrate availability to microbial synthesis and milk production. Journal of Dairy Science,71: 2070–2107.

20- National Research Council. 2001. Nutrient Requirements of dairy cattle.7th ed, National Academy Press. Washington, DC.

21- Olivera, R. M. P. 1998. Use of in vitro gas production technique to assess the contribution of both soluble and insoluble fraction on the nutritive value of forages. Msc thesis, University of Aberdeen.

22- Ordway, R. S., V. A. Ishler, and G. A. Varga. 2002. Effects of sucrose supplementation on dry matter intake, milk yield, and blood metabolites of periparturient Holstein dairy cows. Journal of Dairy Science, 85: 879-888.

23- Orskov, E. R. and I. MacDonald. 1979. The estimate of protein degradability in the rumen from incubatoin measurments wighted according to rate of passage. Journal of Agricultural Science, 92: 499- 503.

24- Rezaii, F., and J. Bayat kouhsar. 2019. Effects of microwaving, and moist and dry heating on ruminal degradability of protein and dry matter in soybean meal. Journal of Livestock Science and Technologies, 2019, 7 (1): 11-19.

25- Sannes, R. A., M. A. Messman, and D. B. Vagnoni. 2002. Form of rumen- degradable carbohydrate and nitrogen on microbial protein synthesis and protein efficiency of dairy cows. Journal of Dairy Science, 85: 900–908.

26- SAS. 2003. SAS User's Guide: Statistics, Version 9.1 Edition. SAS Institute, Cary, NC.

27- Strobel, H. J. and J. B. Russell. 1986. Effect of pH and energy spilling on bacterial versus corn starch or beet pulp fiber diet effects of digestion and intestinal amino acids in dairy cows. Journal of Dairy Science, 76: 2692-2700.

28- Theodorou, M. K., B. A. Williams, M. S. Dhanoa, A. B. McAllan, and J. France. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Journal of Animal Feed Science and Technology, 48: 185-197.

29- Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74: 3583- 3597.

30- Weisbjerg, M. R., T. Hvelplund, and B. M. Bibby. 1998. Hydrolysis and fermentation rate of glucose, sucrose and lactose in the rumen. Journal of Animal Science, 48:12–18.

31- Zhao, X. H., M. G. Jian, Z. Shan, B. F. Chuan, J. L. Chan, J. X. Lan, P. Ke, and R. Q. Ming. 2015. Effects of Degradable Protein and Non-Fibre Carbohydrates on Microbial Growth and Fermentation in the Rumen Simulating Fermenter (Rusitec). Italian Journal of Animal Science, 14 (2):3771.