اثر نیتریک اکساید و آربوسکولار میکوریزا بر برخی صفات فیزیولوژیک گیاه شیرین‌بیان تحت تنش شوری

علوم باغبانی

مقاله 5، دوره 33، شماره 1، خرداد 1398، صفحه 53-64   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jhorts4.v33i1.68893

نویسندگان

آرزو صفرزاده¹؛ گیتی برزین^{* 1}؛ داریوش طالعی²

¹دانشگاه آزاد اسلامی واحد اسلامشهر

²دانشگاه شاهد مرکز تحقیقات گیاهان دارویی

چکیده

قارچ آربوسکولار میکوریزا به عنوان یک کود زیستی در تأمین نیاز غذایی گیاهان و کاهش اثرات تنش­های محیطی بر گیاهان می­تواند مفید باشد. از طرف دیگر، نیتریک اکساید در بسیاری از تنش­های محیطی و غیر محیطی از جمله تنش خشکی و شوری نقش دارد. این پژوهش به منظور بررسی اثر نیتریک اکساید (در دو سطح صفر و 2/0 میلی­مولار) در همزیستی قارچ آربوسکولار میکوریزا بر برخی صفات فیزیولوژیک گیاه شیرین‌بیان تحت سطوح مختلف تنش شوری (صفر، 50، 100، 150 و 200 میلی­مولار) در شرایط گلدانی، به صورت فاکتوریل در قالب طرح بلوک­های کامل تصادفی با سه تکرار اجرا گردید. نتایج نشان داد که اثر متقابل تنش شوری، مایکوریزا و نیتریک اکساید بر میزان فلاونوئید، محتوی پرولین، میزان مالون دی آلدئید و فعالیت آنزیم­های آنتی‌اکسیدان کاتالاز، پراکسیداز و سوپر اکسید دیسموتاز در سطح احتمال یک درصد معنی­دار بود. شوری باعث افزایش میزان مالون دی آلدئید، محتوی پرولین و فعالیت آنزیم­های آنتی‌اکسیدانت (کاتالاز، پراکسیداز و سوپراکسید دیسموتاز) شده است. همزیستی قارچ مایکوریزا در ترکیب با نیتریک اکساید و یا به تنهایی، باعث کاهش میزان فلاونوئید و افزایش محتوی پرولین در هر سطوح از تنش شوری شد. نیتریک اکساید اثر معنی­دار قابل توجهی بر صفات اندازه­گیری شده نداشت ولی در ترکیب با قارچ مایکوریزا مؤثرتر واقع شد. به طور کلی، تنش شوری کلرید سدیمی بر صفات فیزیولوژیکی گیاه شیرین‌بیان اثرات منفی معنی داری داشت(05/0≥p)، ولی کاربرد نیتریک اکساید به همراه قارچ مایکوریزا آربوسکولار بطور معنی داری اثرات منفی ناشی از تنش را تعدیل کرد.

کلیدواژه‌ها

آنزیم های آنتی‌اکسیدانت؛ پراکسیداسیون؛ پرولین؛ فلاونوئید؛ کود زیستی

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