Electromagnetic Field Effects on Production of Salicylic Acid and Phenylalanine ammonia lyase in Tobacco Infected with Potato Virus X (PVX)

پژوهش های حفاظت گیاهان ایران

Article 8, Volume 31, Issue 4 - Serial Number 38, December 2018, Pages 653-661   

Document Type: Research Article

DOI: 10.22067/jpp.v31i4.60067

Authors

amin radmard titkanlo^{* 1}; Saeid Nasrollahnezhad; rahim ahmadvand²; abas rezai asl¹; Farva sadat Mostafavi³

¹Gorgan University of Agricultural Sciences & Natural Resources

²Seed and Plant Improvement Institute

³University of Zabol

Abstract

Introduction: Potato virus x (PVX) is one of the most devastating viruses in the fields of tobacco and no effective method to control the virus has been provided yet. Magnetism is known as one of the environmental stresses that can directly or indirectly affect the plants, especially plants under stress. The magnetic fields can have beneficial affects plants.
Materials and Methods: In the present study to investigate the effects of electromagnetic fields on PVX control, an experiment based on completely randomized design with eleven treatments (10, 50 and 90 mT in a period of 60, 90 and 120 minutes on tobacco seedlings infected with the virus and two control, and the other one is free from analog infected with the virus) in tobacco seedlings carried on six repeats.. After inoculation at the four-leaf stage and after 14 days, the amount of turbidity virus infection rate is calculated and then ensure equality of tobacco seedlings, where the electromagnetic device. 30 days after applying an electromagnetic field analysis of the results of ELISA test two samples were tested with antisera Chndhmsanh of PVX. The amount of salicylic acid and the enzyme phenylalanine ammonia in systemic acquired resistance and plant defense mechanisms are involved, the treatments were measured, for evaluation of HPLC as salicylic acid and phenylalanine ammonia-based assessment of trans-cinnamic acid was used.
Results and Discussion: Statistical analysis ELISA test results revealed that there is a significant difference between treatments 0/05. So that the treatment was a no pollution treatment and 11 who were infected but had not been exposed to the electromagnetic field had a significant difference compared to other treatments, among the treatments that were exposed to the electromagnetic field treatment 8 (100 mT, 120 minutes) showed the lowest OD in ELISA and later treatment 7 (50 mT and 60 minutes) was the lowest OD, the two treatments were observed in less than two OD. If the treatment number 11, OD (2/85) and in pollution-free treatment that was an OD (0/35) was observed. The OD also in other treatments were not exposed between 2.00 to 2.46 were visible. The results showed that the treatment 11 (infected control) actually infected treatment that has not been exposed, and treatment 8 is the most desirable treatment for a reduction in the OD, the difference in the OD are available 0/98. The results suggest that the electromagnetic field decreases the amount of OD in tobacco seedlings are infected with PVX. Researchers said electromagnetic field growth rate and the number of spores grown in three species are affected. In another study, it was found that the impact on dry and wet mycorrhiza. The electromagnetic field decreases witches' broom disease infected lemon trees that were probably due to the decline phytoplasmas in plant tissues. Another study on the antibacterial effects of electromagnetic fields on Serratia marcescens was conducted, which resulted in the inhibition of bacterial growth. The results of salicylic acid and PAL determine the tobacco crop treatments, there are significant differences in the level of 0/05. So that treatment (7) (50 mT, 60 min) the highest salicylic acid and treatment 1 (control non-infected) showed the lowest rates. Also, reviews PAL treatment and treatment (7) most (1) (see non-polluting) had the lowest. In fact, with the arrival of the pathogen to the host salicylic acid and phenylalanine ammonia will increase. But the electromagnetic stress in some treatments of both traits, from infected plants, should take over. However, some treatments less than 11 treatments (infection control) is (but treatment Salicylic acid (4) was less than healthy plant). Interestingly, in both treatments was the highest for both traits in treatments of 7, 6 and 5, respectively. Pre-plant treatments with salicylic acid caused a significant increase in the PAL activity, which indicates the role of these enzymes in plant defense mechanism, the researchers concluded that electromagnetic frequency is very low, possibly because of the resistance of tobacco to TMV and increased Ca2+ signaling pathway involving PAL and ODC hormones in plants.
Conclusions: Comparing results of ELISA plants that had been under different electromagnetic fields the survey showed that 11 infected untreated control treatment without exposure possession was the highest infection. While treatment 8 (100 mT and 120 minutes) and 7 (50 mT for 60 minutes) showed the least amount of turbidity virus. Also in measuring hormone salicylic acid and PAL significant difference between treatments in both studies showed that treatment trait No. 7 in both traits were seen as the highest value. These results suggest a positive effect in reducing turbidity electromagnetic virus, an increase of salicylic acid and phenylalanine ammonia enzymes, hormones and enzymes that are involved in plant defense mechanisms as well.

Keywords

Electromagnetic; Systemic acquired resistance; PVX

References

1- Abdollahi F., Niknam V., Ghanati F., Masroor F., and Noorbakhsh S. N. 2012. Biological effects of weak electromagnetic field on healthy and infected lime (Citrus aurantifolia) trees with Phytoplasma. The Scientific World Journal, 2012.

2- Abdkhani S., Sloki M., Shiri Y., and Yaos A. 2015. The effect of hormone gibberellic acid, jasmonic acid and salicylic acid on gene expression Fnylalanynamvnyalyaz (PAL) at different growth stages. Ocimum basilicum L. Journal of biotech crops, 21: 4-30.

3- Agrios GN. 2005. Plant Pathology (fifth edition). San Diego, California: Elsevier Academic Press. p 237–242.

4- Baure´us Koch CLM., Sommarin M., Persson BRR., Salford LG., and Eberhardt JL. 2003. Interaction between low frequency magnetic fields and cell membranes. Bioelectromagnetics 24:395–402.

5- Bowles D. J. 1990. Defense-related proteins in higher plants. Annual review of biochemistry. 59:873-907.

6- Campbell M.W., and Ellis B.E. 1992. Fungal-elicitor mediated responses in pine cell culture.1.Induction of phenyl propanoid pathway. Plantae 186:409- 417.

7- Cheng G. W., and Breen P. J. 1991. Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. Journal of the American Society for Horticultural Science. 116:865-869.

8- Chandra A., Saxena R., Dubey A., and Saxena P. 2007. Change in phenylalanine ammonia lyase activity and isozyme patterns of polyphenol oxidase and peroxidase by salicylic acid leading to enhance resistance in cowpea against Rhizoctonia solani. Acta Physiologiae Plantarum. 29:361-367.

9- Clark M. F., and Adam A. 1977. Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of general virology. 34:475-483.

10- Davis MS. 1996. Effects of 60 Hz electromagnetic fields on early growth in three plant species and a replication of previous results. Bioelectromagnetics 17:154–161.

11- De Souza A., Garci D., Sueiro L., Gilart F., Porras E., and Licea L. 2006. Pre-sowing magnetic treatments of tomato seeds increase the growth and yield of plants, Bioelectromagnetics, 27:247-257.

12- Durrant WE., and Dong X. 2004. Systemic acquired resistance. Annu Rev Phytopathol 42:185–209.

13- Fischer G., Tausz M., Kock M., and Grill D. 2004. Effects of weak 16.23 Hz magnetic fields on growth parameters of young sunflower and wheat seedlings. Bioelectromagnetics 25:638–641.

14- Galland P., and Pazur A. 2005. Magnetoreception in plants. J Plant Res 118:371–389.

15- Gonzalez-Lamothe R., El Oirdi M., El Rahman T. A., Sansregret R., Bathily H., Bouarab K., and Daayf F. 2009. Cross talk between induced plant immune systems. Molecular Plant-Microbe Interactions, 163.

16- Ghanati F., Morita A., and Yokota H. 2005. Effects of aluminum on the growth of tea plant and activation of antioxidant system. Plant and soil, 276(1-2), 133-141.

17- Grazia T., Francesco B., Lisa L., Patrizia T., G. Lorenzo C., and Lucietta B. 2007. Extremely Low Frequency Weak Magnetic Fields Enhance Resistance of NN Tobacco Plants to Tobacco Mosaic Virus and Elicit Stress-Related Biochemical Activities. Bioelectromagnetics 28: 214-223.

18- Hamidzade S., Kahriz J. S. and Ebadi A. 2014. How to change the content of carbohydrates, total protein Nzym‌Hay planned oxidant effect of salicylic acid in cell death in two wheat cultivars. Crop production, 2: 97-112. (in Persian with English abstract)

19- Hi-Ping L., Rainer F., and Yu C. L. 2001. Molecular evidence for induction of phenylalanine ammonia lyase during Puccinia graminis infection and elicitation in wheat. Can. J. Plant Phatol. 23:286-291.

20- Khosh sokhan M. M., Ghanati F., Zare M. H., Abdolmaleki P., Khorami S. K., Eteadi B., and Vaez zade M. 2004. Effect of Static Magnetic Field on the metabolism of some phenolic compounds in red cabbage plants. Research and development, 70:63-69. (in Persian with English abstract)

21- Kobayashi M., Soda N., Miyo T., and Ueda Y. 2004. Effects of combined DC and AC magnetic fields on germination of hornwort seeds. Bioelectromagnetics 25:552–559.

22- Mardani H., Bayat H., and Azizi M. 2011. Effect of foliar application of salicylic acid on morphological characteristics of cucumber seedlings under drought stress. Journal of Horticultural Science, 25(3): 320-326. (in Persian with English abstract)

23- Mauch F., Mauch‐Mani B., Gaille C., Kull B., Haas D., and Reimmann C. 2001. Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase. The Plant Journal. 25:67-77.

24- Nagy P. 2003. The effect of light and low frequency magnetic fields on microscopic fungi (Doctoral dissertation, Hungarian Academy of Sciences).

25- Nagy P. 2005. The effect of low inductivity static magnetic field on some plant pathogen fungi. Journal of Central European Agriculture, 6(2): 167-172.

26- Paxton J. 1981. Phytoalexins—a working redefinition. Journal of phytopathology. 101:106-109.

27- Piacentini MP., Piatti E., Fraternale D., Ricci D., Albertini MC., and Accorsi A. 2004. Phospholipase C-dependent phosphoinositide breakdown induced by ELF-EMF in Peganum harmala calli. Biochimie 86:343–349.

28- Piatti E., Albertini M. C., Baffone W., Fraternale D., Citterio B., Piacentini M. P., and Accorsi A. 2002. Antibacterial effect of a magnetic field on Serratia marcescens and related virulence to Hordeum vulgare and Rubus fruticosus callus cells. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 132(2): 359-365.

29- Ruzic R., Berden M., and Jerman I. 1998. The effects of oscillating electromagnetic fields on plants. Summary Report, Proc. First World Congress on the Bioeffects of Electricity and Magnetism on the Natural World, Madeira, UK. pp. 1-6.

30- Ruzic R., and Jerman I. 2002.Weak magnetic field decreases heat stress in cress seedlings. Electromagnetic Biol Med 21:69–80.

31- Seo S., Okamotoc M., Iwaia T., Iwanod M., Fukuie K., Isogaid A., Nakajimaf N., and Ohashiet Y. 2000. Reduced levels of chloroplast FtsH protein in tobacco mosaic virus-infected tobacco leaves accelerate the hypersensitive response. Plant Cell 12:917–932.

32- Singh Gill S., and Tuteja N. 2010. Reactive Oxygen Species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48: 909-930.

33- Stange B. C., Rowland R. E., Rapley B. I., and Podd J. V. 2002. ELF magnetic field increase amino acid uptake into Vicia Faba L. roots and alter ion movement across the plasma membrane. Bioelectromagnetics 23:347–354.

34- Takahashi Y., Berberich T., Miyazaki A., Seo S., Ohashi Y., and Kusano T. 2003. Spermine signalling in tobacco: Activation of 222 Trebbi et al. mitogen-activated protein kinases by spermine is mediated through mitocondrial dysfunction. Plant J 36: 820–829.

35- Toiu A., Vlase L., Oniga I., and Tamas M. 2008. HPLC analysis of salicylic acid derivatives from Viola species. Chemistry of Natural Compounds. 44:357-358.

36- Toiu A., Vlase L., Oniga I., Benedec D., and Tãmaş M. 2011. HPLC analysis of salicylic derivatives from natural products. Farmacia. 59:106-112.

37- Walker J. 2005. Fusarium wilt of tomato. The American phytopathological society. Iranian, J. Agric. Sci. 36.

38- Wang J. W., Zheng L. P., Wu J. Y., and Tan R. X. 2006. Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and Taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide, 15(4), 351-358.

39- Zacheo G., Bleve-Zacheo T., Pacoda D., Orlando C., and Durbin R. 1995. The association between heat-induced susceptibility of tomato to Meloidogyne incognita and peroxidase activity. Physiological and molecular plant pathology. 46:491-507.

40- Zeighami nejad R., and Sharifi S. G. 2013. Related gene expression and enzyme activity of the pathogen in the induction of resistance to powdery mildew pumpkin by salicylic acid. Journal of Agricultural Biotechnology, 5(1): 97-110. (in Persian with English abstract)