Effects of Seed Priming on Root Characteristics of Two Barley (Hordeum vulgare L.) Cultivars in Different Levels of Salinity Stress by using Gel Chamber Technique

پژوهشهای زراعی ایران

Article 11, Volume 13, Issue 1 - Serial Number 34, April 2015, Pages 101-108   

Document Type: Research Article

DOI: 10.22067/gsc.v13i1.23869

Authors

H. R Khazaei; A Nezami; B Saadatian^* ; O Armand Pishe

Ferdowsi University of Mashhad

Abstract

The study was conducted to evaluate the effect of seed priming on root growth of two barley cultivars (Hordeum vulgare L.) under salinity stress was performed in phytogel. The treatments included two cultivars of barely (Yusuf and Maquie), levels of seed priming (control, hydro-priming and priming with 4.35 g l-1 of urea) and four levels of NaCl salinity stress (0, 0.2, 0.3 M). The experiment was conducted in Factorial experiment based on a completely randomized design (CRD) with three replications. The results indicated that salinity and seed priming were significantly effects on length, volume, density, fresh and dry weight and rate of root development of barley. Also, the effect of cultivar was significantly in volume, fresh weight and density. Salinity stress reduced length, volume, density, fresh and dry weight and rate of root development. However, the most of barely traits which were affected by seed priming were improved in 0, 0.1 and 0.2 salt Molar levels and priming with urea had the most positive impact on root traits. In the highest salinity level, none of seed priming treatments had not significant effect on root traits. The most effective of Hydro-priming was obtained in the absence of stress. In most of salinity treatments Yusuf cv. in compared with Maquie cv, had more fresh weight and volume root was higher fresh and In general, seed priming could be used as a solution to improve root development of barley seedlings until 0.2 Molar NaCl salinity stress.

Keywords

barley; Hydro-priming; Osmopriming; Phytogel; Sodium Chloride; Urea

References

1- دیانتی تیلکی، ق.، ب. شاکرمی، م. طبری، و ب. بهتری. 1390. اثر NaCl پرایمینگ بر مولفه های جوانه زنی و رشد اولیه بذرهای گونه Festuca ovina L. در شرایط تنش شوری. فصلنامه تحقیقات مرتع و بیابان ایران. 18: 462-452.

2- عبدالرحمنی، ب.، ک. قاسمی گلعذانی، م. ولی زاده، و. فیضی اصل، و ع. توکلی. 1390. اثر پرایمینگ بذر بر روند رشد و عملکرد دانه جو (Hordeum vulgare L.) رقم آبیدر در شرایط دیم. مجله به زراعی نهال و بذر. 27: 129-111.

3- عبدالرحمنی، ب.، ک. قاسمی گلعذانی، م. ولی زاده، و. فیضی اصل، و ع. توکلی. 1388. اثر پرایمینگ بذر بر قدرت رویش و عملکرد دانه جو رقم آبیدر در شرایط دیم. مجله علوم زراعی ایران. 11: 252-237.

4- کافی، م.، ا. برزوئی، م. صالحی، ع. کمندی، ع. معصومی، و ج. نباتی. 1388. فیزیولوژی تنش های محیطی در گیاهان. انتشارات جهاد دانشگاهی مشهد. 502 صفحه.

5- مکی زاده تفتی، م.، ر. فرهودی، و م. راستی فر. 1390. بررسی تاثیر اسموپرایمینگ بر جوانه زنی گیاه بادرنجبویه (Melissa officinalis L.) تحت تنش شوری. فصلنامه تحقیقات گیاهان دارویی و معطر ایران. 27: 586-573.

6- Afkari, A. 2010. The effects of NaCl priming on salt tolerance in sunflower germination and seedling grown under salinity conditions. African J. Biotech, 9: 1764-1770.

7- Ahmadvand, G., F. Soleymani, B. Saadatian, and M. Pouya. 2012. Effects of seed priming on germination and emergence traits of two soybean cultivars under salinity stress. International Res. J. Applied and Basic Sci, 3: 234-241.

8- Anwar, S., M. Shafi, J. Bakht, M. Tariq Jan, and Y. Hayat. 2011. Effect of salinity and seed priming on growth and biochemical parameters of different barely genotypes. African J. Biotech, 10: 15278-15286.

9- Bengough, A. G., D. C. Gordon, H. Al-Menaie, R. P. Ellis, D. Allan, R. Keith, W. T. B. Thomas, and B. P Forster. 2004. Gel observation chamber for rapid screening of root traits in cereal seedlings. Plant and Soil, 262: 63-70.

10- Bose, B, and T. Mishra. 1992. Response of wheat seed to pre sowing seed treatments with Mg (NO3). Annual of Agric Res, 13: 132-136.

11- Bradford, K. J. 1986. Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Horti Sci, 21: 1105-1112.

12- Cavusoglu, K, and K. Kabar. 2010. Effects of hydrogen peroxide on the germination and early seedling growth of barley under NaCl and high temperature stresses. EurAsian J. Bio Sci, 4: 70-79.

13- Cayuela, E., F. Perez-Alfocea, and M. C. Caro. 1996. Priming of seeds with NaCl induces physiological change in tomato plants growth under salt stress. Plant Physiol, 96: 231-236.

14- Chen, K., A. Fessehaie, and R. Arora. 2011. Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and desiccation in Spinacia oleracea L. cv. Bloomsdale: Possible role in stress tolerance. Plant Sci, 182: 420-430.

15- Chiu, K. Y., S. J. Chuang, and J. M. Sung. 2006. Both anti-oxidation and lipid-carbohydrate conversion enhancements are involved in priming-improved emergence of Echinacea purpurea seeds that differ in size. Scientia Horti, 108: 220-226.

16- Demir, I., and H. A. Van De Venter. 1999. The effect of priming treatments on the performance of watermelon (Citrillus lanatus (Thunb.) Matsum. and Nakai) seeds under temperature and osmotic stress. Seed Sci and Technol, 27: 871-875.

17- Ghana, S. G, and W. F. Schillinger. 2003. Seed priming winter wheat for germination, emergence, and yield. Crop Sci, 43: 2135-2141.

18- Hopper, N. W., J. R. Overholt, and J. R. Martin. 1979. Effect of cultivar, temperature and seed size on the germination and emergence of soybeans (Glycine max (L.) Merr.). Ann. Botany, 44: 301-308.

19- Jafar, M. Z., M. Farooq, M. A. Cheema, I. Afzal, S. M. A. Basra, M. A. Wahid, T. Aziz, and M. Shahid. 2011. Improving the Performance of Wheat by Seed Priming Under Saline Conditions. J. Agron and Crop Sci, 14: 1-8.

20- Kaya, M. D., G. Okcu, M. Atak, Y. Cıkılı, and O. Kolsarıcı. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annus L.). Eur. J. Agron, 24: 291-295.

21- Maguire, J. D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigour. Crop Sci, 2: 176-177.

22- Munns, R. 2002. Comparative physiology of salt and water stress. Plant Cell and Environ, 25:239-250.

23- Patade, V. Y., S. Bhargava, and P. Suprasanna. 2009. Halopriming imparts tolerance to salt and PEG induced drought stress in Sugarcane. Agri Ecosys. and Environ, 134: 24-28.

24- Soltani, A., M. Gholipoor, and M. E. Zeinali. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environ. Exp. Botany, 55: 195-200.

25- Wang, H. Y., C. L. Chen, and J. M. Sung. 2003. Both warm water soaking and solid priming treatments enhance anti-oxidation of bitter gourd seeds germinated at sub-optimal temperature. Seed Sci. and Technol, 31: 47-56.

26- Yagmur, M, and D. Kaydan. 2008. Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatments. African J. Biotech. 7: 2156-2162.