The Reaction of Barley Cultivars against Pathotypes of Barley and Wheat Stripe Rust at Seedling and Adult Plant Stages

پژوهش های حفاظت گیاهان ایران

Article 5, Volume 32, Issue 1 - Serial Number 39, March 2018, Pages 71-82   

Document Type: Research Article

DOI: 10.22067/jpp.v32i1.60206

Author

safarali safavi^*

Ardabil Agricultural and Natural Resources Research Center

Abstract

Introduction: Stripe (yellow) rust of barley (Hordeum vulgare L.), caused by Puccinia striiformis f. sp. hordei, is one of the most important diseases of barley in many parts of the world which can cause yield losses due to severe epidemics. Stripe rust of barley is become increasingly important in some parts of Iran due to the cultivation of susceptible cultivars and or appearing new pathotypes. Yield losses of 30 to 70 % occurred in these regions. New, more effective fungicides are available to control yellow rust, but the most efficient, economical, and environmentally friendly approach, is to grow resistant cultivars. It should be noted that the majority of designated Yr-genes are race-specific and therefore become ineffective in combating current pathogen populations due to development of new races. The average lifetime of the genes conferring race-specific resistance is estimated to be five years on the global basis. An alternative procedure for wheat breeders is the use of quantitative resistance. Two types of quantitative resistance, including high-temperature adult-plant (HTAP) resistance and slow rusting resistance, have been intensively investigated. Although several studies have been carried out for assessment of different barley genotypes to yellow rust in Iran, no research has been reported on the screening of barley cultivars for different pathotypes of barley and wheat yellow rusts.
Materials and Methods: In order to, resistance evaluation, seedling and adult plant reactions of 30 barley cultivars were evaluated to yellow rust. The seedling reaction was evaluated in a greenhouse using four barley pathotypes (PSH-51, PSH-84, PSH-85, and PSH-89) and one wheat pathotype (6E150A+, Yr27). The seedling reactions were recorded under greenhouse conditions, based on a 0-to-9 scale. Race population was considered avirulent on the differential set when there were either no symptoms (IT 0) or there were necrotic or chlorotic flecks (IT 1), necrotic or chlorotic blotches without sporulation (IT 2), or necrotic or chlorotic blotches with only a trace to slight sporulation (IT 3 to 4). Race population was considered to be virulent if it caused moderate to abundant sporulation, with or without necrosis or chlorosis (IT 5, 6, 7, 8, or 9).
Adult plant resistance was also evaluated by measuring final rust severity (FRS) and coefficient of infection (CI) under natural infection conditions with two times artificial inoculations. Artificial inoculation, during 2010-2011 cropping years, was carried out by barley and wheat yellow rust inoculum. Percent severity was recorded three times, starting when check reached 50% severity according to the modified Cobb,s scale, and reaction based on Roelfs et al. (1992). The coefficient of infection (CI) was calculated by multiplying disease severity (DS) and constant values of infection type (IT). The constant values for infection types were used based on; resistant (R) =0.1, moderately resistant (MR)=0.25, moderate or moderately resistant to moderately susceptible (M)=0.5, moderately susceptible (MS)=0.75, susceptible (S)=1.
Results and Discussion: Results of evaluations for resistance parameters showed that cultivars Eram, D10, Shori-5, Topper, Afzal, Goharjow and Torsh had susceptible reaction at seedling stage and the high values of FRS and CI, therefore were selected as susceptible cultivars. The cultivars Emir, Asterix and Makouee were resistant both at the seedling and adult plant stages, so they carry race-specific resistance genes against all used-pathotypes. Six cultivars showed resistant at seedling at least against pathotype PSH-85, but moderate or susceptible reactions at adult plant stage in Ardabil. The thirteen cultivars (entries; 4, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 26), was susceptible at the seedling at least against one pathotype, but had moderate (MR, M or MS) reactions at adult plant stages.
Conclusions: The results of the current study showed that the cultivars had a diversity of reactions, ranging from complete resistance to full susceptibility. Most of the evaluated cultivars exhibited moderate or low performance under high disease pressure shown by the susceptible check. The resistance of all categories (including complete resistance, to partial resistance) to stripe rust was observed. The cultivars having the low level of different parameters supposed to be having gene/s for varying degrees of slow rusting resistance or high-temperature adult-plant (HTAP) resistance. Cluster analysis of barley cultivars based on adult plant resistance parameters and seedling infection types revealed different groups/clusters which indicate considerable diversity for a level of resistance of these barley cultivars.

Keywords

Adult plant resistance; barley; Pathotypes; Puccinia striiformis; Seedling resistance

References

1- Ali S., Shah S.J.A., and Ibrahim M. 2007. Assessment of wheat breeding lines for slow yellow rusting (Puccinia striiformis West. tritici). Pakistan Journal of Biological Sciences, 10: 3440-3444.

2- Ali S., Shah S.J.A., Khalil I.H., Rahman H., Maqbool K., and Ullah W. 2009b. Partial resistance to yellow rust in introduced winter wheat germplasm at the north of Pakistan. Australian Journal of Crop Science, 3: 37-43.

3- Ali S., Shah S.J.A., and Rahman H. 2009a. Multi-location variability in Pakistan for partial resistance in wheat to Puccinia striiformis f. sp. tritici. Phytopathologia Mediterrania, 48: 269-278.

4- Bariana H. S., and McIntosh R. A. 1995. Genetics of adult plant resistance in four Australian and the French cultivar Hybrid de Bersee. Plant Breeding, 114: 485-491.

5- Boyd L.A. 2005. Centenary review: can Robigus defeat an old enemy? -Yellow rust of wheat. The Journal of Agricultural sciences, 143: 233-243.

6- Broers L.H.M., Cuesta-Subias X., and Lopez-Atilano R.M. 1996. Field assessment of quantitative resistance to yellow rust in ten spring bread wheat cultivars. Euphytica, 90:9-16.

7- Brown W. M. J., Hill J. P., and Velasco V. R. 2001a. Barley yellow rust in North America. Annual Review of Phytopathology, 39: 367-384.

8- Brown W. M., Hill J. P., and Velasco V.R. 2001b. Identification of barley rust resistance in the Americas: A case study of successful international cooperation. Abstracts of First Regional Yellow Rust Conference for Central & West Asia and North Africa. 8- 14 May 2001, Karaj, Iran. Pages 8-9.

9- Chen X. M. 2005. Epidemiology and control of stripe rust (Puccinia striiformis f. sp. tritici) on wheat. Canadian Journal of Plant Pathology, 27: 314-337.

10- Chen X. M., and Line R.F. 2003. Identification of genes for resistance to Puccinia striiformis f. sp. hordei in 18 barley genotypes. Euphytica, 129: 127-145.

11- Chen X. M., Line R. F., and Leung H. 1995. Virulence and polymorphic DNA relationships of Puccinia striiformis f. sp. hordei to other rusts. Phytopathology, 85: 1335-1342.

12- Dadrezaie S. T., Lababidi S., Nazari K., Mohammadi E., Afshari F., Alo F., Shams-Bakhsh M., and Safaei N. 2013. Molecular genetic diversity in Iranian populations of Puccinia triticina, the causal agent of wheat leaf rust. American Journal of Plant Science, 4: 1375-1386.

13- Dehghani H., and Moghaddam M. 2004. Genetic analysis of latent period of stripe rust in wheat seedlings. Journal of Phytopathology, 122: 325-330.

14- Dubin H. J., and Stubbs R. W. 1986. Epidemic spread of barley stripe rust in South America. Plant Disease, 70: 141-144.

15- Esfandiari E. 1947. Les rouilles de cereales en Iran [Cereal rusts in Iran]. Applied Entomology and Phytopathology, 4: 67–76.

16- Flor H.H. 1956. The complementary genetic systems in flax and flax rust. Advanced Genetics, 8: 29-54.

17- Golegaonkar P. G., Wellings C. R., Singh D., and Park R.F. 2013. Genetic and molecular analysis of resistance to a variant of Puccinia striiformis in barley. Journal of Applied Genetics, 54: 1-9.

18- Gulati S. C., Varma N. S., Jdin K. B. L., and Chaudhary H. B. 1988. Sources of resistance to yellow rust (Puccinia striiformis West.) in barley. Indian Journal of Genetics and Plant Breeding, 48: 271-274.

19- Hei N., Shimelis H. A., Laing M., and Admassu B. 2015. Assessment of Ethiopian wheat lines for slow rusting resistance to stem rust of wheat caused by Puccinia graminis f. sp. tritici. Journal of Phytopathology, 163: 353-363.

20- Herrera-Fossel S.A., Singh R.P., Huerta-Espino J., Crossa, J., Djurle A., and Yuen J. 2007. Evaluation of slow rusting resistance components to leaf rust in CIMMYT durum wheats. Euphytica, 155: 361-369.

21- Holtz M. D., Kumar K., Zantinge J. L., and Xi K. 2013. Virulence phenotypes of Puccinia striiformis in Alberta from 2009-2011. Canadian Journal of Plant Pathology, 35: 241-250.

22- Holtz M. D., Kumar K., Zantinge J. L., and Xi K. 2014. Genetic diversity of Puccinia striiformis from cereals in Alberta, Canada. Plant Pathology, 63: 415-424.

23- Hovmøller M. S. 2007. Sources of seedling and adult plant resistance to Puccinia striiformis f. sp. Tritici in European wheats. Plant Breeding, 126: 225-233.

24- Hovmøller M. S., Sørense C. K., Walter S., and Justesen A. F. 2011. Diversity of Puccinia striiformis on Cereals and Grasses. Annual Review of Phytopathology, 49: 197-217.

25- Jin Y. 2011. Role of Berberis spp. as alternate hosts in generating new races of Puccinia graminis and P. striiformis. Euphytica, 179: 105-108.

26- Johnson R. 1988. Durable resistance to yellow (stripe) rust in wheat and its implications in plant breeding. In: Simmonds N. W. and Rajaram S. (eds.) Breeding strategies for resistance to the rusts of wheat, p. 63-75. Mexico, D. F. CIMMYT.

27- Kilpatrick R. A. 1975. New cultivars and longevity of rust resistance, 1971-1975. U.S Agricultural Research Service, ARS-NE 64, 20 pp.

28- Kumar K., Holtz M. D., Xi K., and Turkington T. K. 2012. Virulence of Puccinia striiformis on whaet and barley in central Alberta. Canadian Journal of Plant Pathology, 34: 551-561.

29- Line R. F. 2002. Stripe rust of wheat and barley in North America: A retrospective historical review. Annual Review of Phytopathology, 40: 75-118.

30- Line R. F., and Chen X.M. 1995. Success in breeding for and managing durable resistance to wheat rusts. Plant Disease, 79: 1254-1255.

31- Line R., and Qayoum A. 1992. Virulence, aggressiveness, evolution, and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968-87. USDA-ARS Tech. Bull. 1788. 44 pp.

32- Mathur A. K., and Siradhana B. S. 1990. Screening of indigenous and exotic barley germplasm against Indian races of yellow rust. Indian Journal of Mycology and Plant Pathology, 20: 182.

33- McIntosh R. A., Wellings C. R., and Park R. F. 1995. Wheat Rusts: An Atlas of Resistance Genes. Csiro, Australia, 200 pp.

34- Milus E.A., and Line R.F. 1986. Gene action for inheritance of durable, high– temperature, adult plant resistances to stripe rust in wheat. Phytopathology 76: 435-441.

35- Moshiri Sh. 1995. Determination of resistance sources to common barley diseases in Khorasan. Proceeding of the 12th Iranian plant protection congress, 28 Aug- 1Sep, Karaj, Iran. pp: 53. (In Persian).

36- Nazari K., Torabi M., Hassanpour-Hosnei M., Kashani A., Hoshyar R., and Ahmadian-Moghaddam M. S. 2000. Evaluation of resistance to yellow rust in advanced wheat lines suitable for dryland areas at seedling and adult-plant stages. Seed and Plant, 16: 252-262. (In Persian).

37- Niks R. E., Heyzen S., Szabo L. J., and Alemu S. K. 2013. Host status of barley to Puccinia coronate from couch grass and P. striiformis from wheat and brome. European Journal of Plant Pathology, 136: 393-405.

38- Parlevliet J.E. 1979. Components of resistance that reduce the rate of epidemic development. Annual Review of Phytopathology, 17: 203-222.

39- Pathan A. K., and Park R. F. 2006. Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica, 149: 327-342.

40- Peterson R. F., Campbell A. B., and Hannah A. E. 1948. A diagrammatic scale for estimating rust intensity of leaves and stems of cereals. Canadian Journal of Research, 26: 496-500.

41- Roelfs A. P., and Huerta- Espino J. 1994. Seedling resistance in Hordeum to barley stripe rust from Texas. Plant Disease, 78: 1046-49.

42- Roelfs A. P., Singh R. P., and Saari E. E. 1992. Rust diseases of wheat: Concepts and Methods of Diseases Management. Mexico, D.F.CIMMYT.81.pp.

43- Safavi S. A., Afshari F., and Yazdansepas A. 2013a. Effective and ineffective resistance genes to wheat yellow rust during six years monitoring in Ardabil. Archives of Phytopathology and Plant Protection, 46: 774-780.

44- Safavi S. A., and Afshari F. 2012a. Quantitative resistance of some Elite wheat lines to Puccinia striiformis f. sp. tritici, Archives of Phytopathology and Plant Protection, 45:740-749.

45- Safavi S. A., Atahossaini S. M., and Torabi M. 2004. Study on resistance reaction of elite barley lines to Puccinia striiformis f. sp. hordei. Proceeding of the Second regional yellow rust conference for Central & West Asia and North Africa, 22-26 March, Islamabad, Pakistan. pp:

46- Safavi S. A., Babai-Ahari A., Afshari F., and Arzanlou M. 2012. Effect of yellow rust on yield components of barley cultivars with race-specific and slow rusting resistance to yellow rust. Archives of Phytopathology and Plant Protection, 45:1488–1498.

47- Safavi S. A., Jahani-Jelodar Y., and Ebrahimnejad Sh. 2013b. Virulence patterns of barley yellow rust and effective resistance genes to Puccinia striiformis in three parts of Iran, Archives of Phytopathology and Plant Protection, 46: 903-910.

48- Safavi S.A., and Afshari F. 2012b. Identification of resistance to Puccinia striiformis f. sp. tritici in some elite wheat lines. Journal of Crop Protection, 1: 293-302.

49- Safavi S.A., and Mohammadzadeh J. 2013. Race non-specific resistance to yellow rust in some promising wheat lines. Cereal Research, 3: 197-209. (In Persian)

50- Safavi S.A., Babai Ahari A., Afshari F., and Arzanlou M. 2014. Virulence genes and pathotypes of Puccinia striiformis f. sp. hordei, causing yellow rust on barley in some areas of Iran. Seed and Plant Improvement Journal, 30: 733-760.

51- Saleem K., Arshad H. M. I., Shokat S., and Manzo B. 2015. Appraisal of wheat germplasm for adult plant resistance against stripe rust. Journal of Plant Protection Research, 55: 405-414.

52- Sandoval-Islas J. S., Broers L. H. M., Vivar H., and Osada K. S. 1998. Evaluation of quantitative resistance to yellow rust (Puccinia striiformis f. sp. hordei) in the ICARDA/CIMMYT barley breeding program. Plant Breeding, 117: 127-130.

53- Sandoval-Islas J.S., Broers L.H.M., Mora-Aguilera G., Parlevliet J.E., Osada K.S. and Vivar H.E. 2007. Quatitative resistance and its components in 16 barley cultivars to yellow rust, Puccinia striiformis f. sp. hordei. Euphytica, 153:295-308.

54- Schultz T. R., and Line R. F. 1992. High– temperature, adult plant resistances to wheat stripe rust and effects on yield components. Agronomy Journal, 84: 170-175.

55- Shah S. J. A., Muhmmad M., and Hussain S. 2010. Phenotypic and molecular characterization of wheat for slow rusting resistance against Puccinia striiformis Westend. f. sp. tritici. Journal of Phytopathology, 158: 393-402.

56- Singh K. V., Singh G. P., Singh P. K., and Aggarwal H. R. 2017. Assessment of slow rusting resistance components to stripe rust pathogen in some exotic wheat germplasm. Indian Phytopathology, 70: 52-57.

57- Singh R. P., Huerta-Espino J., Bhavani S., Herrera-Foessel S. A., Singh D., Singh P. K., Velu G., Mason R. E., Jin Y., Njau P., and Crossa J. 2011. Race non-specific resistance to rust diseases in CIMMYT spring wheats. Euphytica, 179:175–186.

58- Singh R.P., Huerta-Espino J., and William H.M. 2005. Genetics and breeding for durable resistance to leaf and stripe rusts in wheat. Turkish Journal of Agriculture and Forestry, 29: 121-127.

59- Stubbs R. W., Prescott J. M., Saari E. E., and Dubin H. J. 1986. Cereal Disease Methodology Manual. CIMMYT: Mexico, D. F. 46pp.

60- Tariq-Khan M., and Irfan Ul-Haque M. 2011. Elite-II synthetic hexaploid wheats as a potential source of resistance against yellow rust. Archives of Phytopathology and Plant Protection, 44:1165-1170.

61- Wan A. M., and Chen X. M. 2012. Virulence, frequency, and distribution of races of Puccinia striiformis f. sp. tritici and Puccinia striiformis f. sp. hordei identified in the United States in 2008 and 2009. Plant Disease, 96: 67-74.

62- Wang M. N., and Chen X. M. 2013. First report of Oregon grape (Mahonia aquifolium) as an alternate host for the wheat stripe rust pathogen (Puccinia striiformis f. sp. tritici) under artificial inoculation. Plant Disease, 97: 839.

63- Welling C. R., Burdon G. G., McIntosh R. A., Wallwork H., Raman H., and Murrary G. M. 2000. A new variant of Puccinia striiformis causing stripe rust on barley and wild Hordeum species in Australia. Plant Pathology, 49: 803.

64- Zhao J., Wang L., Wang Z., Chen X., Zhang H., Yao J., Zhan G., Chen W., Huang L., and Kang Z. S. 2013. Identification of Eighteen Berberis species as alternate hosts of Puccinia striiformis f. sp. tritici and virulence variation in the pathogen isolates from natural infection of barberry plants in China. Phytopathology, 103: 927-934.