نقشه‌یابی QTL‌های کنترل‌کننده صفات فنولوژیک جو تحت شرایط نرمال و تنش خشکی

پژوهشهای زراعی ایران

مقاله 4، دوره 13، شماره 2 - شماره پیاپی 38، تیر 1394، صفحه 256-268   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.22067/gsc.v13i2.49672

نویسندگان

براتعلی فاخری^* ؛ لیلا مهراوران

دانشگاه زابل

چکیده

به‌منظور مکان‌یابی نواحی ژنومی کنترل‌کننده صفات فنولوژیک جو تحت شرایط نرمال و تنش خشکی آزمایشی در سال زراعی 90-1389 با 72 لاین هاپلوئید مضاعف به‌همراه والدین آنها (استپتو و مورکس) در مزرعه تحقیقاتی دانشکده کشاورزی دانشگاه زابل، در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در دو شرایط آبیاری نرمال و تنش خشکی اجرا گردید. صفات فنولوژیک روز تا جوانه‌زنی، روز تا پنجه‌دهی، روز تا ساقه‌دهی، روز تا سنبله‌دهی، روز تا گلدهی، روز تا شیری شدن دانه، روز تا خمیری شدن دانه، روز تا رسیدگی دانه و دوره پر شدن دانه اندازه‌گیری شد. تجزیه QTL به روش مکان‌یابی فاصله‌ای مرکب (CIM) با استفاده از نسخه 5/2 نرم افزار QTL کارتوگرافر انجام شد. نقشه لینکاژی نشانگرهای مولکولی جو مرکب از 327 نشانگر RFLP با طول 3/1226 و متوسط فاصله 75/3 سانتی‌مورگان بود. اثر ژنوتیپ برای کلیه صفات مورد مطالعه معنی‌دار (01/0P

کلیدواژه‌ها

استپتو؛ تنش کم آبی؛ مورکس؛ هاپلوئیدهای مضاعف

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