Effects of Plant Density and Nitrogen on Physiological Growth Indices, Yield Components and Yield of Black Seed (Nigella sativa L.) as a Medicinal Plant

بوم شناسی کشاورزی

Article 5, Volume 12, Issue 4 - Serial Number 46, December 2021, Pages 635-650   

Document Type: Scientific - Research

DOI: 10.22067/agry.2020.37577

Authors

Abdollah Mollafilabi^{* 1}; Hossein Moodi²

¹Research Institute of Food Science and Technology, Mashhad, Iran

²Department of Agronomy, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Abstract

Introduction
There is an increasing interest to produce medicinal plants as the demand for these natural products is also increasing in the world. Since the middle of the twentieth century, after identifying the negative side effects of chemical drugs, medicinal plants have been replaced by chemical drugs in many cases.  Due to the climatic diversity, Iran has a high potential for the production of medicinal plants, however, only a very small portion of the world’s market for medicinal plants is allocated to Iran. Considering the possibility of negative effects of chemicals on the quantity and quality of medicinal plants, it is necessary to use ecological principles. On the other hand, it seems that the cultivation of medicinal plants along with other crops would reduce weed and pest population due to the allelopathic properties of these plants. Cultivation of medicinal and aromatic plants has several advantages like higher net returns per area unit, low incidence of pests and diseases, improvement of degraded and marginal soils, longer shelf life of final products and foreign exchange earning potential. Black seed (Nigella sativa L.) is a widely used medicinal plant throughout the world which belongs to Ranunculaceae family. This experiment was carried out to evaluate physiological growth indices, yield and yield components of black seed affected by nitrogen rate and plant density under Mashhad climate conditions.
Materials and Methods
This experiment was conducted at the Agricultural Research Station of the Ferdowsi University of Mashhad, located in 10 km south-east of Mashhad (59° 36ˊ East, 36° 15ˊ N, 985 meters above sea level) during the 2016-2017 growing season. The experiment was conducted as factorial layout based on a randomized complete block design with two factors and four replications. Treatments were four plant densities (60, 120, 180 and 240 plants.m^-2) and four nitrogen rates (0, 50, 100 and 150 kg N per ha as urea). Studied traits were leaf area index (LAI), dry matter accumulation (DM), crop growth rate (CGR), relative growth rate (RGR), plant height, yield components (such as number of branches per plant, number of follicles per plant, number of seeds per follicle and 1000-seed weigh), seed yield, straw yield and biological yield. SAS software was used to analyze the data and the comparison of means was done using Duncan multiple range test at 5% probability level. Charts were also drawn using Excel software.
Results and Discussion
The results indicated that the plant density and nitrogen rates affects leaf area index (LAI), dry matter accumulation (DM), crop growth rate (CGR), relative growth rate (RGR), plant height, yield components (such as number of branches per plant, number of follicles per plant, number of seeds per follicle and 1000-seed weigh), seed yield, straw yield and biological yield of black seed. The highest and lowest leaf area index was observed in 100 kg N and control, respectively. The fast period of vegetative growth, leaf area index and dry matter accumulation were observed at flowering stage with a small decline afterwards until physiological maturity. Also, crop growth rate reached to its peak in flowering stage followed by a decreasing trend afterwards. In addition, number of branches decreased sharply at high densities. The highest seed yield was observed from 150 kg N per ha (895.7 kg.ha^-1) and the lowest was for control (689.9 kg.ha^-1). Biological yield had high correlation with straw yield (r²=0.99) and seed yield (r²=0.97).
 
Conclusion
Agronomic management strategies had significantly effect on growth, yield, and yield components of black seed. Generally, plant density and nitrogen rates are two effective techniques for agronomic management of the medicinal plants. Further, investigations on quantity and quality of medicinal plants including black seed in association with agronomic operations will provide additional information.

Keywords

Leaf area index; Number of follicles per plant; Seed yield; Straw yield

References

Akbarinia, A., Daneshian, J., and Mohammadbeigi, F., 2006. Effect of nitrogen fertilizer and plant density on seed yield, essential oil and oil content of Coriandrum sativum L. Iranian Journal of Medicinal and Aromatic Plants 22(4): 410-419. (In Persian with English Summary)

Amirmoradi, S., and Rezvani Moghaddam, P., 2011. Effect of plant density and time of nitrogen application on morphological, phenological characteristics, yield and yield components of black cumin (Nigella sativa L.). Journal of Horticulture Science 25(3): 251-260. (In Persian with English Summary)

Atta, M.B., 2003. Some characteristics of (Nigella sativa L.) seed cultivated in Egypt and its lipid profile. Food Chemistry Journal 83(1): 63-68.

Babayan, V.K., Kootungal, D., and Halaby, G.A., 1978. Proximate analysis, fatty acid and, amino acid composition of (Nigella sativa L.) seed. Journal of Food Science 43: 1315–1319.

Baloch, A.W., Soomro, A.M., Javed, M.A., Ahmad, M., Bughio, H.R., and Bughio, M.S., 2002. Optimum plant density for high yield in rice (Oriza sativa L.). Asian Journal of Plant Science 1(2): 114-116.

Blackman, V.H., 1919. The compound interest law and plant growth. Annals of Botany 33: 353-360.

Dadvand Sarab, M.R., Naghdi Abadi, H.A., Nasri, M., Makizadeh, M., and Omidi, H., 2008. Variations of essential oil contents and yield of Basil (Ocimum basilicum L.) affected by plant density and nitrogen. Medicinal Plants 3(27): 60-70. (In Persian with English Summary)

Das, A.K., Sadhu, M.K., and Som, M.G., 1991. Effect of N and P levels on growth and yield of black cumin (Nigella sativa L.). Horticulture Journal 4: 41-47.

Erkan, N., Ayranci, G., and Ayranci, E., 2008. Antioxidant activities of rosemary (Rosmarinus officinalis L.) extract, blackseed (Nigella sativa L.) essential oil, carnosic acid, rosmaric acid and sesamol. Food Chemistry 110: 76–82.

 Eydizadeh, K., Mahdavi Damghani, A., Sabahi, H., and Soufizadeh, S., 2010. Effects of Integrated application of biofertiliser and chemical fertilizer on growth of maize (Zea mays L.) in Shushtar. Journal of Agroecology 2(2): 292-301. (In Persian with English Summary)

Gardner, F.P., Pearce, R.B., and Mitchell, R.M., 1985. Physiology of Crop Plants. Iowa State University Press, 327 pp.

Ghilavizadeh, A., Taghi Darzi, M., and Rejali, F., 2012. Influence of plant growth promoter bacteria and plant density on yield and yield components and seed yield of ajowan (Carum copticum). International Journal of Agriculture and Crop Sciences 4(17): 1255-1260.

Ghobadi, M., Jahanbin, S., Motalebi Fard, R., and Parvizi, K., 2011. The effect of biological phosphate fertilizers to yield and yield components of potato. Sustainable Agriculture and Production Science 21(2): 117-130. (In Persian with English Summary)

Ghosh, D.K., Roy, K., and Malic, S.C., 1981. Effect of fertilizers and spacing on yield and other characters of black cumin (Nigella sativa L.). Indian Agriculture 25: 191-197.

Habibzadeh, Y., Mamghani, R., and Kashani, A., 2006. Effects of plant density on grain yield and some morpho-physiological traits in three mungbean (Vigna radiate L.) genotypes under Ahvaz conditions. Iranian Journal of Crop Science 8(1): 66-78. (In Persian with English Summary)

Hamisi, M., Sefidkon, S., Nasri, M., and Lebaschi, M.H., 2012. Effects of different amounts of nitrogen, phosphor and bovine fertilizers on essential oil content and composition of Tanacetum parthenium L. Iranian Journal of Medicinal and Aromatic Plants 28(3): 299-410. (In Persian with English Summary)

Hokmalipour, S., and Seyedsharifi, R., 2014. Effect of seed inoculation with plant growth promoting rhizobacteria (PGPR) affected by different levels of nitrogen and phosphorus fertilizers on the yield and some physiological parameters of barley (Hordeum vulgar L.). Iranian Journal of Field Crops Research 12(4): 822-833. (In Persian with English Summary)

Hosseinpour, M., Pirzad, A., Habibi, H., and Fotokian, M.H., 2011. Effect of biological nitrogen fertilizer (Azotobacter) and plant density on yield, yield components and essential oil of anise. Journal of Sustainable Agriculture and Production Science 2(1): 69-86. (In Persian with English Summary)

Hussain, A., Nadeem, A., Ashraf, I., and Awan, M., 2009. Effect of weed competition periods on the growth and yield of black seed (Nigella sativa L.). Pakistan Journal of Weed Science Research 15: 71–81.

Jahan, M., Amiri, M.B., and Ehyaei, H.R., 2012. Absorption and radiation use efficiency of sesame affected by biological fertilizers in a low-input agroecosystem. Iranian Journal of Field Crops Research 2(10): 435-447. (In Persian with English Summary)

Khan, S.A., and Chattergee, B.N., 1982. Fertilizer use by Nigella sativa L. in West Bengal. Indian Journal of Agricultural Sciences 52: 389-387.

Lebaschi, M.H., and Sharifi, E., 2004. Application of physiological growth indices for suitable harvesting of Hypericum perforatum. Pajouhesh and Sazandegi (65): 65-75. (In Persian with English Summary)

Manaffee, W.F., and Kloepper, J.W., 1994. Applications of plant growth promoting rhizobacteria in sustainable agriculture. In C.E. Pankburst, B.M. Doube, V.V.S.R. Gupta and P.R. Grace, (Eds.) p. 23-31. CSLRO, Publication. East Melbourne: Australia.

McMaster, G.S., and Wilhelm, W.W., 1998. Soil biota management in sustainable farming by shoots. Is soil temperature better than air temperature for predicting winter wheat phenology. Agronomy Journal 90(5): 602-607.

Mehta, B.K, Pandit, V., and Gupta, M., 2009. New principle from seeds of Nigella sativa. Natural Product Research 23: 138–148.

Mohammadian, M., Rezvani Moghaddam, P., Zarghani, H., and Yanegh, A.J., 2013. Morphological and physiological evaluation of intercropping of three elite sesames. Iranian Journal of Field Crops Research 7(2): 685-693. (In Persian with English Summary)

Morteza, E., Akbari, G.A., Modares Sanavi, S.A.M., Aliabadi Farahani, H., Foghi, B., and Abdoli, M., 2010. The effects of sowing date and planting density on some of the growth characteristics of Valerian (Valeriana officinalis L.) and didrovaltrate content. Iranian Journal of Medicinal and Aromatic Plants 25(4): 495-503. (In Persian with English Summary)

Mosavi, S.G.R., Segatoleslami, M.J., and Pooyan, M., 2012. Effect of planting date and plant density on yield and seed yield components of Plantago ovata L. Iranian Journal of Medicinal and Aromatic Plants 27(4): 699-681. (In Persian with English Summary)

Mosavi, S.H., Fathi, G., and Dadgar, M.A., 2005. Planting date and density on growth, yield components and yield of red beans. Fist Pulse National Conference, 20-21^st November. Ferdowsi University of Mashhad, Mashhad, Iran. (In Persian)

Mostafavi, M.J., 2014. The effect of chemical and biological fertilizers on quantitative and qualitative yield of sesame (Sesamum indicum L.) in Mashhad climate condition. MSc. Thesis, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. (In Persian with English Summary)

Oozooni Doji, A., Esfahani, M., Samee Zadeh Lahiji, H., and Rabiee, M., 2008. The effect of planting pattern and plant density on the growth and radiation use efficiency of two cultivars of without petals and with petals rapeseed. Iranian Journal of Field Crop Science 9(4): 382-400. (In Persian with English Summary)

Pirzad, A.R., 2007. Effect of irrigation and plant density on some physiological traits of german chamomile (Matricaria chamomilla L.). Ph.D. Thesis, University of Tabriz, Tabriz, Iran 195 pp. (In Persian with English Summary)

Pop, G., Pirsan, P., Mateoc-sirb, N., and Mateoc, T., 2007. Influence of technological elements on yield quantity and quality in marigold (Calendula officinalis L.) cultivated in cultural conditions of Timisoara. 1^st International Scientific Conference on Medicinal, Aromatic and Spice Plants: Nitra, p. 20-23.

Raee, Y., Ghasemi Golozaani, A., Javanshir, A., Aliyaari, H., and Mohammadi, A., 2008. Effect of plant density on intercropping of soybean and sorghum. Journal of Sciences and Technology of Agriculture and Natural Resources 12(45): 35-44. (In Persian with English Summary)

Rahmani, N., Jalali-Yekta, A., Taherkhani, T., and Daneshian, J., 2010. Effect of different levels of plant density and nitrogen on essential oil yield of marigold (Calendula officinalis L.). Journal of Crop Ecophysiology 2(1): 347-354. (In Persian with English Summary)

Ramadan, F.M., and Morsel, J.T., 2003. Analysis of glycolipids from black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.) and niger (Guizotia abyssinica Cass.) oilseeds. Food Chemistry 80: 197–204.

Rassam, G.A., Naddaf, M., and Sefiskon, F., 2007. Effect of planting date and plant density on yield and seed yield components of Anise (Pimpinella anisum L.). Pajouhesh and Sazandegi 75: 127-133. (In Persian with English Summary)

Rezvani Moghaddam, P.,  Seyyedi, S.M., and Azad, M., 2016. Effect of fertilizer managements on yield and yield components of black seed (Nigella sativa L.). Journal of Agroecology 8(4): 598-611. (In Persian with English Summary)

Saberali, F., Sadat Nouri, S.A., Hejazi, A., and Zand, E., 2007. Influence of plant density and planting pattern of corn on its growth and yield under competition with common lambesquarterrs (Chenopodium album). Journal of Research Production 74: 143-152.

Seyed Sharifi, R., and Nemati, A.R., 2014. Influence of rates and nitrogen fertilizer application timing on yield and some growth physiological indices of corn (Zea mays L.). Journal of Crop Production and Processing 3(10): 49-67. (In Persian with English Summary)

Sharifi Ashoorabadi, E., Matin, A., Lebaschi, M.H., and Abbaszadeh, B., 2004. Effects of nitrogen application methods on yield of Melissa (Melissa officinalis). Iranian Journal of Medicinal and Aromatic Plants Research 20(3): 369-376. (In Persian with English Summary)

Singh, S., Buttar, G.S., Singh, S.P., and Brar, D.S., 2005. Effect of different dates of sowing and row spacing on yield of fenugreek (Trigonella foenum-gracum). Journal of Medicine and Aromatic Plant Science 27(4): 629-640.

Sobhani, G., Golchin, A., and Shekari, F., 2014. Effects of different levels of nitrogen and induced-NaCl stress on yield and growth indices of tomato. Journal of Science and Technology of Greenhouse Culture (Soilless Culture Research Center) 5(3): 49-63. (In Persian with English Summary)

 Tarigholeslami, M., Zarghami, R., Mashhad, M., Bojar, A., and Oveisi, M., 2012. Effect of nitrogen fertilizer and water deficit stress on physiological indices of corn (Zea mays L.). Journal of Agronomy and Plant Breeding 8(1): 161-174. (In Persian with English Summary)

Toncer, O., and Kizil, S., 2004. Effect of seed rate on agronomic and technologic characters of Nigella sativa L. International Journal of Agriculture and Biology 6(3): 529-532.

Vahidipour, T.H., Vahidipour, H.R., Baradaran, R., and Seqhatoleslami, M.J., 2013. Effect of irrigation and nitrogen fertilizer on grain yield and essential oil percentage of medicinal plant ajowan. International Journal of Agronomy and Plant Production 4(5): 1013-1022.

Zafarian, F., Agha Alikhani, M., Rahimian Mashhadi, H., Zand, E., and Rezvani, M., 2007. Yield and yield components response of corn/soybean intercrop to simultaneous competition of redroot pigweed and jimson weed. Iranian Journal of Weed Science 3(1): 39-58. (In Persian with English Summary)