Effects of Cycocel on Morphological Traits, Nitrogen and Potassium Content of Basil Plants under Water Stress Conditions

پژوهشهای زراعی ایران

Article 6, Volume 14, Issue 2 - Serial Number 42, July 2016, Pages 343-353   

Document Type: Research Article

DOI: 10.22067/gsc.v14i2.39851

Authors

A Rezaei Estakhroeih^{* 1}; B Babaei²

¹Shahid Bahonar University of Kerman

²University of Zabol

Abstract

Introduction
Basil (Ocimum basilicum) is an annual plant which belongs to the Lamiaceae family. It used as a drug, spice and fresh vegetable. Drought stress is one of the important limiting factors of plant growth. Water stress has significant effects on morphological and biochemical characteristics of purple Basil. As the soil water content decreases, the plant height, stem diameter, number and area of leaves, leaf area index (LAI), herb yield and leaf chlorophyll contents (a,b and total chlorophyll) decrease, as well. However, the amounts of anthocyanin and proline increase. Cycocel (CCC) which chemically called chlormequat chloride is an alkylating agent and a quaternary ammonium salt.. Cycocel is used as plant growth regulator. Application of Cycocel increases the number of siliques/ plant, seed yield and dry matter produced of oilseed rape. Cycocel application decreases the plant height and increases the yield level and protein percentage of seed in faba bean. This research was performed to investigate the effect of Cycocel on morphological characteristics, the percentage of nitrogen and potassium of basil plants under drought stress conditions
Materials and Methods
This research has been conducted in the research station of Shahid Bahonar University of Kerman with 56o 58' E longitude, 30o 15' N latitude, and 1753.8 altitudes. According to the regional information from 1952 to 2005, the average temperature was 17.1 o C, the average rainfall was 154.1 mm, the average annual relative humidity is 32%, and the climate of Kerman according to De Martonne method is semiarid. A split plot experiment based on RCBD with three replications was employed. Three levels of irrigation, including 50 (severe stress) (I3), 75 (mild stress) (I2) and 100 (full irrigation) (I1) percent of crop water requirement were assigned to the main plots while five levels of Cycocel application (zero (control), 500, 1000, 1500, 2000 milligrams per litre) were assigned to the sub-plots. Cultivation was carried out in March and harvested in late June. In this study the plant height, fresh and dry weight of root and shoot, root to shoot dry weight ratio, percentage of nitrogen and potassium, leaf relative water content and leaf area index were measured. For its statistical calculation, SAS (v. 9.1) software and for the comparison of the averages the Duncan’s test was used. The level of possibility used in the analysis was five percent.
Results and Discussion
The result of the analysis of variance of measured traits has shown in Table 1. Data analysis of variance (Table 1) shows different levels of irrigation, Cycocel and irrigation with Cycocel interaction were significant at the level of one percent on plant height. Effects of different irrigation treatments and Cycocel concentration on fresh and dry weight of shoot were significant at one and five percent, respectively. However, the effect of irrigation and Cycocel interaction on shoot dry weight was not significant. Root fresh and dry weight were influenced by irrigation levels (Table 1). The effect of irrigation and Cycocel interaction on root dry weight, percent of nitrogen and dry weight of root to shoot ratio was significantat one percent. The effect of irrigation on root to shoot dry weight ratio was not significant. Moreover, the effects of Cycocel treatments on root to shoot dry weight ratio were significant at the five percent level. The effect of irrigation, Cycocel on percent of nitrogen and potassium was significant and potassium decreased with the increasing of water stress.
The highest percentage of potassium (2.26%) belonged to the full irrigation and the concentration of 2000 milligrams per litre Cycocel and the lowest belonged to the interaction of severe stress and zero, 500 and 1000 milligrams per litre Cycocel. Irrigation and Cycocel had a significant influence on leaf relative water content at the level of one percent.
Conclusions
According to the results which obtained through this study, in basil plant, root fresh and dry weight, shoot fresh and dry weight, leaf area index, leaf relative water content and plant height reduced by increasing drought stress. Root-shoot dry weight ratio increased in response to increasing stress in all the plants which treated by Cycocel . Cycocel treatments reduced plant height, root fresh and dry weight, shoot dry weight and LAI. Leaf relative water content and shoot fresh weight increase with increasing the concentrations of Cycocel. The interaction of 2000 milligram Cycocel per litre with irrigation has the greatest effect on the traits.

Keywords

basil; Cycocel; Drought stress; Minerals; morphological traits

References

1- Asadollahi, A., Mirza, M., Abbaszadeh, B., Azizpour, S., and A. Keshavarzi. 2013. Comparison of Essential oil from Leaves and Inflorescence of three Basil (Ocimum basilicum L.) Populations under Drought Stress. International Journal of Agronomy and Plant Production 4(10):1764-1767.

2- Bakhtiari, B., Liaghat, A., KHalili, A., and Kjanjani, M. J. 2010. Evaluation of Two Hourly Combination Models for Estimation of Grass Reference Evapotranspiration (Case study: Climate of Kerman). Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil Science 13(50):13-26. (In Persian with English abstract).

3- Costa Franca, M. G. 2000. Differences in growth and water relations among Phaseolus vulgaris cultivars in response to induced drought stress. Environmental and Experimental Botany 43:227-237.

4- Forouzandeh, M., Fanoudi, M., Arazmjou, E. and H. Tbiei. 2012. Effect of drought stress and types of fertilizers on the quantity and quality of medicinal plant Basil (Ocimum basilicum L.). Indian Journal of Innovations and Developments 1(10):734-737.

5- Goldani, M. 2012. Effect of Irrigation Intervals on Some Morphophysiological Traits of Basil (Ocimum basilicum L.) Ecotypes. Journal of Iranian Field Crop Research 10(2):412-420. (In Persian with English abstract)

6- Hassani, A., Omidbaiji, R. and H, Heidari Sharifabad. 2003. Effect of different soil moisture levels on growth, yield and accumulation of compatible solutes in Basil (Ocimum basilicum). Iranian Journal of Soil and Waters Sciences, 17(2):219-229. (In Persian with English abstract).

7- Hojjati, M., Etemadi, N., B. Baninasab. 2010. Effect of Paclobutrazol and Cycocel on Vegetative Growth and flowering of rudbeckia. Journal of Horticultural Sciences 24(2): 122-127. (In Persian with English abstract).

8- Kjeldal, S. E. 1998. An investigation of several psychological factors impinging on the Perception of fresh fruits and vegetables. Unpublished PhD Thesis. University of New England, Australia. 448 p.

9- Memari, H. R., Tafazoli, E., Kamgar-Haghighi, A., Hassanpour, A. and N. Yarami. 2011. Effects of Water Stress and Cycocel as a Growth Retardant on Growth of Two Olive Cultivars. Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil Science 15(55):1-11. (In Persian with English abstract).

10- Mirzaii Abdolyosefi, A. A., Jafari Haghighi, B. and Y. Emam. 2011. Effect of planting density and chlormequat chloride on growth and yield of wheat (Triticum aestivum v. Cross Azadi). Journal of Plant Ecophysiology, 3(8):1-15. (In Persian with English abstract).

11- Moshrefi araghi, A., naderi, R., babalar, M. and M. Taheri. 2014. Effect of different spraying levels of cycocle on vegetative growth and flowering of poinsettia pot plant (Euphorbia pulcherrima Willd.). Journal of Science and Technology of Greenhouse Culture 5(17):73-84. (In Persian with English abstract).

12- Movahhedi Dehnavi, M., Ranjbar, M., Yadavi, A. R. And B. Kavusi. 2011. Effect of cycocel on proline, soluble sugars, protein, oil and fatty acids of flax (Limum usitatissimum M.) plants under drought stress in a pot trial. Environmental Stresses in Crop Sciences 3(2):129-138. (In Persian with English abstract).

13- Omidbaigi, R. 2005. Approaches to the production and processing of medicinal plants. Behnashr Publications, Mashhad. 1:347. (In Persian with English abstract).

14- Prakash, M. and K. Ramachandran. 2000. Effects of chemical ameliorants on stomata frequency and water relations in Brinjal (Solanum melongena L.) under moisture stress conditions. Journal of Agronomy and Crop Science 185(4):237-239.

15- Rabbi Angourani, H., Mortazavi, S. N., Rabiei, V. and E. Zangahani. 2013. Effect of Cycosel and Naphthalene Acetic Acid on some Vegetative Characteristics and on Essential Oil Yield in Rose - Scented Geranium (Pelargonium graveolens L.cv Bourbon). Iranian Journal of Horticuitural Sciences (Iranian Journal of Agricultur Sciences) 44(2):209-216. (In Persian with English abstract).

16- Ramroudi, M. and A. R. Khamr. 2013. Interaction effects of salicylic acid spraying and different irrigation levels on some quantity and quality traits, and osmoregulators in basil (Ocimum basilicum). Journal of applied Research of Plant Ecophysiology 1(1):19-31. (In Persian with English abstract).

17- Rezaei Estakhroeih, A. 2012. Effect of deficit irrigation and partial rootzone drying on the yield, yield components and water use efficiency of corn. PhD Thesis. University of Shahid Chamran, Ahvaz, Iran. 173 p. (In Persian with English abstract).

18- Riccardi, F., Gazeau, D. and M. Vienne. 1998. Protein changes in response to progressive water deficit in maize plant Physio 117:1253-1263.

19- Ritchie, S. W., and H. T. Nguyen. 1990. Leaf water content and gas exchange parameters of two wheat genotypes differing in drought resistance. Crop Science 30: 105-111.

20- Sharif, S., Saffari, M. and Y. Emam. 2007. The Effect of Drought Stress and Cycocel on Barley Yield (Cv. Valfajr). Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil Science 10 (4):281-291. (In Persian with English abstract).

21- Tahami Zarandi, S. M. K., Rezvani Moghaddam, P. and M. Jahan. 2014. Effects of various organic and chemical fertilizers on growth indices of Basil (Ocimum basilicum). Agroecology 5(4):363-372. (In Persian with English abstract).

22- Tolbert, N. E. 1960. (2-Chloroethyl) trimethyl-ammonium chloride and related compounds as plant growth substances. 1. Chemical structure and bioassay. Jour. Boil. Chem 235:475-479.