بررسی نسبی تولید آنزیم لیپاز و کیتیناز قارچ‌های جدا شده از نماتد سیستی چغندرقند در شرایط آزمایشگاهی

پژوهش های حفاظت گیاهان ایران

مقاله 1، دوره 34، شماره 1 - شماره پیاپی 47، فروردین 1399، صفحه 1-13   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jpp.v34i1.63886

نویسندگان

اعظم سلیمانی؛ عصمت مهدیخانی مقدم^* ؛ حمید روحانی

دانشگاه فردوسی مشهد

چکیده

در این تحقیق فعالیت آنزیمی لیپاز و کیتیناز قارچ­های همراه نماتد سیستی چغندرقند (Heterodera schachtii) جمع­آوری شده از استان خراسان رضوی مورد مطالعه قرار گرفت. به این منظور در سال زراعی 95-94، حدود 300 جدایه قارچی از سیست، ماده و تخم و لارو نماتد سیستی چغندرقند جداسازی گردید. جدایه­های مذکور پس از خالص­سازی، مورد بررسی­های ماکروسکوپی، میکروسکوپی و مولکولی قرار گرفتند و با استفاده از کلیدهای معتبر شناسایی شدند. از میان آن­ها شش گونه شامل Simplicillium lanosoniveum، Penicillium chrysogenum، Colletotrichum gloeosporioides، Neonectria macrodidyma، Fusarium oxysporum و Alternaria chlamydospora شناسایی شدند. در بررسی نسبی فعالیت آنزیم لیپاز، قارچ C .gloeosporioides بیشترین میزان تولید این آنزیم را نشان داد و در بررسی نسبی فعالیت کیتیناز، بیشترین تولید این آنزیم مربوط به قارچ P .chrysogenum بود.

کلیدواژه‌ها

چغندر قند؛ نماتد سیستی؛ قارچ؛ لیپاز؛ کیتیناز

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