اثر سرب بر مقدار گلومالین هیفی و ریشه ‏ای واکنش ‏پذیر با آنتی ‏بادی مونوکلونال و بردفورد در شرایط کشت درون شیشه‏ ای و گلدانی

آب و خاک

مقاله 3، دوره 30، شماره 2 - شماره پیاپی 46، تیر 1395، صفحه 605-617   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jsw.v30i2.47802

نویسندگان

الهام ملک زاده^{* 1}؛ جعفر مجیدی²؛ ناصر علی اصغرزاد¹؛ جلال عبدالعلی زاده³

¹دانشگاه تبریز

²علوم پزشکی تبریز

³دانشگاه علوم پزشکی تبریز

چکیده

گلومالین به عنوان گلیکوپروتئین شناخته شده در قارچ‏های میکوریزی شاخه گلومرومایکوتا و راسته گلومرال با دو روش بردفورد و آنتی‏بادی مونوکلونال اندازه‏گیری می‏شود. با این پیش فرض که تنش ناشی از فلز سمی منجر به افزایش بیان و تولید گلومالین به عنوان پروتئین شوک حرارتی می‏گردد، پژوهشی جهت مطالعه و مقایسه مقدار گلومالین تولیدی در شرایط کشت گلدانی در همزیستی قارچ رایزوفاگوس ایرگولاریز با گیاه شبدر سفید (Trifolium repens L.) و کشت درون شیشه‏ای در همزیستی ریشه‏های تراریخت هویج (Daucus carota L.) با همان قارچ تحت تنش سرب طراحی گردید. در کشت درون شیشه‏ای با افزایش غلظت سرب (0، 01/0، 1/0 و 1 میلی‏مولار Pb+2) مجموع درصد فراوانی هیف و اسپور کاهش یافت درحالی که پروتئین واکنش‏پذیر بردفورد و پروتئین واکنش پذیر با آنتی بادی در بخش هیفی افزایش یافت. در کشت گلدانی با افزایش غلظت سرب (0، 150، 300 و 450 میکرومولار Pb+2) درصد طول ریشه‏ کلنی‏شده نسبت به شاهد افزایش نشان داد. به طور کلی با افزایش غلظت سرب، پروتئین واکنش‏پذیر بردفورد و پروتئین واکنش پذیر با آنتی‏بادی در بخش هیفی و ریشه‏ای کشت گلدانی افزایش یافت. آنتی بادی مونوکلونال واکنش متقاطع ناچیزی با پروتئین‏های ریشه‏های غیرمیکوریزی نشان داد. بنابراین تولید گلومالین از طریق ممانعت از تغییر شکل پروتئین‏های مهم و حیاتی گیاه به عنوان پروتئین شوک حرارتی، ساز و کار حفاظتی قارچ های میکوریز آربوسکولار در همزیستی با گیاه برای کاهش تنش ناشی از سرب می‏باشد.

کلیدواژه‌ها

الایزا؛ بردفورد؛ قارچ میکوریز آربوسکولار؛ گلومالین

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