بررسی اثر تلقیح میکوریزی و پرایمینگ بذر بر برخی خصوصیات کمی و کیفی عدس (.Lens culinaris L)

آب و خاک

مقاله 3، دوره 30، شماره 3 - شماره پیاپی 47، شهریور 1395، صفحه 817-828   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jsw.v30i3.40474

نویسندگان

محسن آذرنیا¹؛ عباس بیابانی¹؛ عبدالطیف قلی زاده^{* 1}؛ حمیدرضا عیسوند²؛ ابراهیم غلامعلی پور علمداری³

¹دانشگاه گنبد کاووس

²دانشگاه لرستان

³دانشگاه گنبدکاووس

چکیده

به منظور ارزیابی عکس العمل عدس به پرایمینگ بذر (بدون پرایمینگ بذر، هیدروپرایمینگ، 100 پی‌پی‌ام اسید جیبرلیک، 100 پی‌پی‌ام اسید سالیسیلیک، 100 پی‌پی‌ام اسید جیبرلیک + 100 پی‌پی‌ام اسید سالیسیلیک) و تلقیح میکوریزایی خاک (عدم تلقیح به عنوان شاهد، تلقیح با گلوموس اینترارادیسز و تلقیح با گلوموس موسه )، آزمایش فاکتوریل بر پایه طرح بلوک کامل تصادفی در چهار تکرار در مزرعه تحقیقاتی دانشگاه گنبد کاووس در سال 93-1392 به اجرا درآمد. صفت‌های مورد اندازه‌گیری شامل؛ طول ریشه، تعداد گره تثبیت کننده نیتروژن، غلظت فسفر اندام هوایی، غلظت فسفر دانه، جذب فسفر دانه، جذب فسفر اندام هوایی، عملکرد دانه و عملکرد بیولوژیک بودند. تجزیه واریانس داده‌ها نشان داد که تیمارهای مختلف تلقیح میکوریزی، پرایمینگ و اثر متقابل آن‌ها تاثیر معنی‌داری بر صفات مورد مطالعه نظیر طول ریشه، تعداد گره‌های تثبیت کننده نیتروژن، غلظت فسفر اندام هوایی، غلظت فسفر دانه، میزان جذب فسفر اندام هوایی، میزان جذب فسفر دانه، عملکرد زیستی و عملکرد دانه داشتند. در این مطالعه بیشترین طول ریشه (5/39 سانتی‌متر)، تعداد گره تثبیت کننده نیتروژن (114)، جذب فسفر اندام هوایی (1/12 کیلوگرم در هکتار) در کاربرد توام تلقیح با گلوموس اینترارادیسز + 100 پی‌پی‌ام جیبرلین بدست آمد. درحالی که بیشترین غلظت فسفر اندام هوایی (24/0 درصد) و جذب فسفر دانه (8/22 کیلوگرم در هکتار) در کاربرد توام میکوریزای گلوموس موسه و هیدروپرایمینگ مشاهده شد. پرایمینگ هورمونی با اسید سالیسیلیک نسبت به شاهد و سایر تیمارهای پرایمینگ هورمونی سبب افزایش معنی‌داری در عملکرد دانه‌ای و بیولوژیک شد. هیدروپرایمینگ در سه سطح کود زیستی اثر مثبت معنی‌داری بر عملکرد دانه داشت که با اسید سالیسیلیک غیر معنی‌دار ولی با سایر تیمارهای پرایمینگ اختلاف معنی‌داری داشت.

کلیدواژه‌ها

اسید جیبرلیک؛ جذب فسفر؛ گره‌های تثبیت کننده نیتروژن؛ گلوموس موسه؛ هیدروپرایمینگ

مراجع

1- Abdel-Fattah G.M., and Asrar A.W.A. 2012. Arbuscular mycorrhizal fungal application to improve growth and tolerance of wheat (Triticum aestivum L.) plants grown in saline soil. Acta Physiologiae Plantarum 34:267–277.

2- Alahdadi I., Tajik M., Iran-nejad H., and Armandpisheh O. 2009. The effect of biofertilizer on soybean seed vigor and field emergence. Journal of Food, Agriculture & Environment Vol.7 (3&4): 420 – 426.

3- Alimadadi A., Jahansouz M.R., Besharati H., Tavakkol-Afshari R., and Tavakkoli M. 2010. Effect phosphate solubilizing micro-organisms, mycorrhiza and seed priming on the nodulation in chickpea crop (Cicer arietinum L.). Journal of Soil Research (Soil Science and Water) 24(1): 43-53. )in Persian with English abstract(.

4- Alimadadi A., Jahansouz M.R., Besharati H., Tavakkol-Afshari R., and Tavakkoli M. 2011. Evaluating the effects of biofertilizers and seed priming on chickpea (Cicer arietinum L.) seed quality. Journal of Food, Agriculture & Environment Vol.9 (2) :362 – 365.

5- Amer G.A., and Utkhede R.S. 2000. Development of formulation of biological agents for management of root rot of lettuce and cucumber. Canadian Journal of Microbiology, 46: 809–816.

6- Andre S., Galiana A., Le Roux C., Prin Y., Neyra M. and Duponnois R. 2004. Ectomycorrhizal symbiosis enhanced the efficiency of inoculation whit two Bradyrhizobium strains and Acacia holosericea growth. Mycorrhiza. 15 (5): 357-364.

7- Antunes P. M., Deaville D. and Goss M. J. 2005. Effect of two AMF life strategies on the tripartite symbiosis whit Bradyrhizobium japonicum and soybean. Mycorrhiza. Issue: Online First, Published online: 16 December 2005.

8- Arif M., Tariq Jan M., Mian I.A., Khan S.A., Philip H., and Harris, D. 2014. Evaluating the Impact of Osmopriming Varying with Polyethylene Glycol Concentrations and Durations on Soybean. international journal of agriculture & biology. 16(2):359–364.

9- Ashraf M., Karim F., and Rasul E. 2002. Interactive effects of gibberellic acid (GA3) and salt stress on growth, ion accumulation and photosynthetic capacity of two spring wheat (Triticum aestivum L.) cultivars differing in salt tolerance. Plant Growth Regulator., 36(1): 49-59.

10- Atkinson S., Berta G., and Hooker J.E. 1994. Impact of mycorrhizal colonization on root architecture, root longevity and the formation of growth regulators. In Gianinazzi S. and H. Schüepp (Eds.), Impact of Arbuscular Mycorrhizas on Sustainable Agriculture and Natural Ecosystems (pp. 47-60). Basel, Switzerland: Birkhäuser Verlag.

11- Azarnia M., and Eisvand H.R. 2013 (a). Effects of Hydro and Hormonal Priming on Yield and Yield Components of Chickpea in irrigated and rain-fed conditions. 6 (4): 1-18. )in Persian with English abstract(.

12- Azarnia M., and H.R. Eisvand. 2013 (b). Priming is a method for improve seed quality for increase growth and yield crop. Research achievements for field and horticulture crops. 2: 277-287. )in Persian with English abstract(.

13- Badr E. A., Wali A.M., and Gehan A.A. 2013. Effect of Sowing Dates and Biofertilizer on Growth Attributes, Yield and its Components of Two Faba Bean (Vicia faba L.) Cultivars. World Applied Sciences Journal 28 (4): 494-498.

14- Beck D. P., Materon L. A. and Afandi F. 1993. Practical Rhizobium-legume technology manual. ICARDA, Aleppo, Syria.

15- Benton J.J. 2001. Laboratory guid for conducting soil test and plant analysis. 363 pp.USA. CRC Press.

16- Berta G., Fusconi A. and Hooker J. E. 2002. Arbuscular mycorrhizal modifications to plant root systems: scale, mechanisms and consequences. In Gianinazzi, S., H. Schüepp, J. M. Barea and K. Haselwandter (Eds.), Mycorrhiza Technology in Agriculture, from Genes to Bioproducts (pp. 71-85). Basel, Switzerland: Birkhäuser Verlag.

17- Bethlenfalavy G.J., Schreiner R.P., Mihara K.L., and McDaniel H. 1996. Mycorrhizae, biocides, and biocontrol. 2. Mycorrhizal fungi enhance weed control and crop growth in a soybean-cocklebur association treated whit the herbicide bentazon. Applied Soil Ecology. 3 (3): 205-2014.

18- Bhattacharjee S., and Sharma G.D. 2012. Effect of Dual Inoculation of Arbuscular Mycorrhiza and Rhizobium on the Chlorophyll, Nitrogen and Phosphorus Contents of Pigeon Pea (Cajanus cajan L.). Advances in Microbiology. 2, 561-564.

19- Bianciotto V., Andreotti S., Balestrini R., Bonfante P., and Perotto S. 2001. Extracellular polysaccharides are involved in the attachment of Azospirillum brasilense and Rhizobium leguminosarum to arbuscular mycorrhizal structures. European Journal of Histochemistry, 45: 39-49.

20- Biswas J.C., Ladha J.K., and Dazzo F.B. 2000. Rhizobia inoculation improves nutrient uptake and growth of low land rice. Soil Science Society of America Journal, 64: 1644–1650.

21- Cardoso Irene M., and Kuyper T. W. 2006. Mycorrhizas and tropical soil fertility. Agric. Ecosys. Envirron. 116: 72-84.

22- Casenave E.C., and Toselli M.E. 2007. Hydropriming as a pre-treatment for cotton germination under thermal and water stress conditions. Seed Science Technology, 35:88–98.

23- Cheng Z. and Bradford K. J. 1999. Hydrothermal time analysis of tomato seed germination responses to priming treatments, Journal of Experimental Botany. 33, 89-99.

24- Demir Kaya M., Okcu Gamze Atak M., Cikili Y., and Kolsarici O. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Europe Journal Agronomy, 24: 291-295.

25- Dodd J. 2000. The role of arbuscular mycorrhizal fungi in agro-natural ecosystems. Outlook on Agriculture. 29 (1): 63-70.

26- Eisvand H.R., Tavakkol-Afshari R., Sharifzadeh F., Madah Arefi H., and Hesamzadeh Hejazi S. M. 2008. Improvement of physiological quality of deteriorated tall wheat grass (Agropyron elongatum Host) seeds by hormonal priming for control and drought stress conditions. Iranian Journal of Field Crop Science. 1 (39) :53-65. )in Persian with English abstract(.

27- Eisvand H.R., Alizadeh M.A., and Fekri A. 2010. (a). How hormonal priming of aged and non aged seeds of bromgrass affects seedling physiological characters. Journal of New seeds.11: 52-64.

28- Eisvand H. R., Tavakkol-Afshari R., Sharifzadeh F., Madah Arefi H., and Hesamzadeh Hejazi S.M. 2010. (b). Effects of hormonal priming and drought stress on activity and isozyme profiles of antioxidant enzymes in deteriorated seed of tall wheatgrass (Agropyron elongatum Host). Seed Science and Technology. 38,280-297.

29- Eisvand H.R., Azarnia M., Nazarian Firozabadi F., Sharafi R. 2011. (a). Effect of Priming by gibberllin and abcsisic acid on emergence and some seedling physiological characters of Chickpea (Cicer arietinum L.) under dry and irrigated conditions. Iranian Journal of Field Crop Science. 42(4): 789-797. )in Persian with English abstract(.

30- Eisvand H.R., Shahrosvand S., Zahedi B., Heidari S., and Afroughe SH. 2011. (b). 'Effects of hydro‐priming and hormonal priming by gibberellin and salicylic acid on seed and seedling quality of carrot (Daucus carota var. sativus)'. Iranian Journal of Plant Physiology. 1 (4), 233‐239.

31- FAO. 2013. FAO Year Book. FAO Publication, (http://faostat.fao.org/site/).

32- GHassemi-Golezani K., Farshbaf-Jafari S., and SHafagh-Kolvanagh J. 2011. Seed Priming and Field Performance of Soybean (Glycine max L.) in Response to Water Limitation. (Available online at www.notulaebotanicae.ro). Notulae Botanicae Horticulture Agro-botanici Cluj-Napoca. 39(2):186-189.

33- GHassemi-Golezani K., Jabbarpour-Bonyadi Z., Shafagh-Kolvanagh J. Nikpour-Rashidabad N. 2013. Effects of Water Stress and hydro-priming duration on field performance of lentil. International Journal of Farming and Allied Sciences. 2(21):922-925.

34- Ghollarata M. 2005. The impact of mycorrhizal inoculation on berseem clover yield and nutrient uptake with different soil salinity and phosphorus levels. MSc. Thesis. Shahrekord univ. Iran.

35- Harrier L.A., and Watson C.A. 2004. The potential role of arbuscular mycorrhizal (AM) fungi in the bioprotection of plants against soil-borne pathogens in and/or other sustainable farming systems. Pest Management Science. 60 (2):149-157.

36- Harris D. B.S.R., aghuwanshi G.S., Gangwar S.C., Singh K.D., Joshi A., Rashid H., and Hollington P.A. 2001. participatory evaluation by farmers of on farm seed priming in wheat in india Nepal and Pakistan .Experimental Agriculture, 37:403-415.

37- Iqbal M., and Ashraf M. 2006. Wheat seed priming in relation to salt tolerance, growth, yield and level of free salicylic acid and polyamines. Annals of Botany. 43(4): 250-259.

38- Kaur S., Gupta A.K., and Kaur N. 2005. Seed priming Increases Crop yield possibly by Modulating enzymes of Sucrose metabolism in chickpea. Journal of Agronomy and Crop Science. 191: 81-87.

39- Khoshkhouy M., Sheibani B., and Roohani Tafazzoli A. 1999. Gardening Principles (Principles of Garden Supplies). Shiraz University Press. 566 pages. (in Persian).

40- Kormanik P.P., and McGraw A.C. 1982. Quantification of vesiculararbuscular mycorrhizae in plant roots. In: Schenck NC (ed)Methods and Principles of Mycorrhizal Research. The American Phytopathological Society. St. Paul, pp 37–45

41- Kurle J.E., and Pfleger E.I. 2005. Arbuscular- mycorrhizal fungus spore population responded to conversions between low-input and conventional management practices in a corn-soybean rotation. Agronomy Journal. 56 (3): 467-476.

42- McDonald M.B. 2000, Seed priming, Black, M., Bewley J.D., (Eds.), Seed Technology and Its Biological Basis, Sheffield Academic Press, Sheffield, UK, 287–325.

43- Mohammadi M., Moghadam H., Majnon Hossini N., Ahmadi A., and Khavazi K. 2011. the effect of phosphorus bio-fertilizer and chemical fertilizer on yield and yield components of two cultivar of lentil in different irrigation. 42 (4): 845-855. )in Persian with English abstract(.

44- Mosse B. 1986. Mycorrhiza in a sustainable agriculture. Biological Agriculture and Horticulture, 3: 191-209.

45- Page A.L. 1982. Methods of soil analysis. Part 2. 2nd ed. American Society of Agronomy, Madison, WI. 1159 pp.

46- Parera C.A., and Cantliffe D.J. 1994. Pre-sowing seed priming. Hort Rev 16:109-139.

47- Parsa M. and Bagheri A. 2008. Pulses. Jihad-e Daneshgahi Mashhad Publisher. (in Persian).

48- Patade V.Y., Bhargava S. and Suprasanna P. 2009. Halopriming imparts tolerance to salt and PEG induced drought stress in sugarcane. Agriculture Ecosystem Environmental. 134:24–28.

49- Poshtvareh R., Mirshekari B., and Mohammadi S. 2011. Sustainable Production of Corn (Zea mays) by Seed Inoculation with Mycorrhiza Strains. Middle-East Journal of Scientific Research 10 (2): 164-167.

50- Sadeghian S.Y., and Yavari N. 2004. Effect of warer-deficit stress on germination and early seedling growth in sugar beet. Journal of Agronomy and Crop Science. 190: 138-144.

51- Sannazzaro A.I., Ruiz O.A., Alberto E.O., and Menendez A.B. 2006. Alleviation of salt stress in lotus glaber byGlomus intradices.Plant Soil. 285:279-287.

52- Senaratna T., Touchell D., Bunn E., and Dixon K. 2000. Acetyl salicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regulate. 30: 157-161.

53- Subramanian K.S., and Charest C. 1997. Nutritional, growth, and reproductive responses of maize (Zea mays L.) to arbuscular mycorrhizal inoculation during and after drought stress at tasselling. Mycorrhiza. 7 (1): 25-32.

54- Varma A., and Hock B. 1999. Mycorrhiza: Structure, Function, Molecular Biology and Biotechnology. Springer microbiology book, Berlin. ISBN: 3-540-63981-0.

55- Watson C.A., and Harrier L. A. 2003. The role of arbuscular mycorrhizal fungi in sustainable cropping system. Advance Aagronomy. 79: 185-225.

56- Zaidi A., Khan M.S., and Amil M. 2003. Interactive effects of Rhizotrophic microorganisms on yield and nutrient uptake of chickpea (Cicer arietinum L.). European Journal of Agronomy, 19: 15-21.

57- Zaidi A., and Khan M.S. 2006. Co-inoculation Effects of Phosphate Solubilizing Microorganisms and Glomus fasciculatum on Green Gram-Bradyrhizobium Symbiosis. Turkish Journal of Agriculture and Forestry. 30: 223-230.

58- Zaki R.N., and Radwan T.E. 2011. Improving wheat grain yield and its quality under salinity conditions at a newly reclaimed soil by using different organic sources as soil or foliar applications. Journal of Applied Sciences Research. 7: 42-55.