The Effects of Carboxin-thiram on Associative Relationships between Azospirillum Species and Wheat (Chamran Cultivar)

آب و خاک

Article 3, Volume 30, Issue 6 - Serial Number 50, January 2016, Pages 1941-1953   

Document Type: Research Article

DOI: 10.22067/jsw.v30i6.33522

Authors

F. rejali^* ; A. Esmaelzad; K. Saghafi; V. hemati

Soil and Water Research Institute, Agriculture Research, Education and Extension Organization

Abstract

Introduction: Biofertilizers have been identified as alternative to chemical fertilizers to increase soil fertility and crop production in sustainable farming systems. One of the most useful kind of biofertilizers include plant growth promoting rhizobacteria (PGPR). Azospirillum is an associative rhizobacteria which can be very useful for plants such as wheat. It can help plant by fixing nitrogen through biological way, causing root development, plant strength improvement in primary phases, causing germination percent increment, improving plant tolerance in stress situations (drought, salinity, soil compaction and pathogens), secreting plant promoting hormones like cytokinin, Oxin and finally yield increment will be observable. Modern agriculture largely relies on the extensive application of agrochemicals, including inorganic fertilizers and pesticides. Although pesticides are important, their effects on nontarget organisms are of great concern because this poses a risk to the entire ecological system. The fungicides may also adversely affect the soil microflora, especially the types of microorganisms that can applied to seeds as bacterial inoculants. Considering useful effects of plant growth promoting rhizobacteria especially Azospirillum on Wheat, this study was done in order to survey interaction effects between fungicide and available biofertilizers in Iran market.
Materials and Methods: Effect of carboxin tiram in 2 levels (applied, non-applied) as fungicide, on efficacy of wheat plant (Chamran Cultivar) and final crop yields under association conditions with 5 Azospirillum species (A.brasilense, A.lipoferum, A.halopraeferense, A.irakense, A.sp) using powdery and liquid formulation were studied in a greenhouse test for four months in Soil and Water Research Institute.At first some properties of used soil, including soil texture, pH, EC,organic carbon and available soil K, P, Fe, Zn, Mn and Cu were measured by laboratory methods.Nutrient Broth medium were used for bacterial inoculum production with 108 bacterial cound per ml in final suspention. Using factorial experiment in a Completely Randomized Design (CRD), 2 bacterial inoculants factors (5 inoculation level and a non-inoculation level), CarboxinThiram fungicide levels (applied, non-applied) and two inoculants formulations (liquid and powdery) with four replicates per treatment and a total of 96 experimental units (pots), the most effective contribution of different species of Azospirillum bacteria with Chamran wheat varieties were evaluated in the presence of the fungicide. Studied Parameters included number of tillers, node interface, flag length, number of grains per spike, grain weight per spike, shoots wet weight, 1000 grain weight and shoot dry weight of wheat plant. Data were analyzed with SASS and Excel softwares. The comparison was done by Tukey test.
Results and Discussion: Regarding ANOVA table (table 2), liquid and powdery formulations of Azospirillum with different species had significant effect on 8 of 11 studied traits including number of tillers, plant hight, spike length, node interface, flag length, number of spikes per square meter , grain weight in spike, shoot wet weight, shoot dry weight. Fungicide had effect on 2 traits such as number of grain per spike and grain weight spike independently. Bacteria and fingicide interaction had significant effect on number of tillers, node interface, flag length, number of grain per spike, shoot dry weight (p< 0.01) and shoot wet weight (p< 0.05).
Numeric comparsion between similar treatments in presence and absence of fungicide, it can be concluded that although fungicide presence had no significant positive effectson studied traits, it did not have any negative effects eigther.Even it could increase traits quantity by affecting on bacteria. Also, regarding to Table 5, comparing fungicide effect on bacteria with studied trait in two formulations, it can be concluded that fungicide presence with bacteria was effective on quantity of some trait in powdery formulation and some in liquid formulation.These findinds may be the result of fungicide effects in controlling soil born pathogens in compatable treatments with used bacterial inoculums.
Conclusions: As final result, using A.lipoferum with both formulations and A.halopraeferense with powdery formulation, because of high compatibility with carboxin thiram fungicide can be advised in case of fungicide application. This advice can have good effects on functional traits such as number of tillers, grain weight in spike and shoot dry weight.To consider the effects of environmental conditions on the final results we propose to do this experiment in field scale in some Iranian provinces with different climatic conditions.The use of different concentrations of carboxin tiram and also different kinds of PGPR and other fungicides must be consider in future experiments.

Keywords

Azospirillum; Carboxin-thiram; Inoculant formulation; wheat

References

1- Ali Ehyayee M., and Behbahani Zadeh A. 1994. Description of soil chemical analysis methods. Soil and Water Research Institute. Vol. 893. 128p. (in Persian)

2- Bashan Y., Harrison K. and Witimoyer R. E. 1990. Enhanced growth of wheat and soybean plants inoculated with Azospirillum brasilense of mineral uptake. Applied and Environmental Microbiology. 56: 769-775.

3- Bertholet J., and Clark W. 1985. Effect of trifluralin and metribuzin on faba bean growth, development and symbiotic nitrogen fixation. Canadian Journal of Plant Sciences. 65:9-20.

4- Casida J. E. 2009. Pest toxicology: the primary mechanisms of pesticide action. Chemical Research in Toxicology. 22(4): 609-619.

5- Dobereiner J., and Day J. M. 1976. Associative symbiosis in tropical grasses: characterization of microorganisms and dinitrogen fixing sites. In Proceedings of the First International Symposium on Nitrogen Fixation. Edited by W.E. Newton and C.J. Nyman.Washington State University Press, Pullman, Wash. pp. 518.538.

6- Ebhin Masto R., Chhonkar P. K., Singh D., and Patra A. K. 2006. Changes in soil biological and biochemical characteristics in a long-term field trial on a sub-tropical inceptisoil. Soil Biology and Biochemistry. 38: 1577-1582.

7- http://damoonagri.persianblog.ir

8- Kalia A, and Gupta R. P. 2004. Disruption of soil foodweb by pesticides. Indian Journal of Ecology. 31(2):85-92.

9- Kloepper J. W., Lifshitz R., and Zablotwicz R. M. 1989. Free living inocula for enhancing crop producing. Trends in Biotechnology. 7: 39-43.

10- Lucy M., Reed E., and Glick B. R. 2004. Application of free living plant growth-promoting rhizobacteria. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. 86: 1–25.

11- Lynch J. M. 2002. Resilience of the rhizosphere to anthropogenic disturbance. Biodegradation. 13: 21-27.

12- Magalhaes F. M., Baldani J. I., Souto S. M., Kuykendall J. R., and Dobereine J. 1983. A new acid-tolerant Azospirillum species. The Brazilian Academy of Sciences. 55: 417-430.

13- Mano D. M. S., Buff K., Clausen E., and Langenbach T. 1996. Bioaccumulation and enhanced persistente of the acaricide dicofol by Azospirillum lipoferum. Chemosphere. 33: 1609-1619.

14- Mubben F. 2004. Biochemical and molecular approaches to study the effect of pesticides (Fungicide) on root-associated bacteria in wheat. PhD. thesis, Faculty of Science, The University of Agriculture Faisalabad, Pakistan.

15- Mubeen F., Aslam A., Sheikh M. A., Iqbal T., Malik S., Hameed K. A., Hafeez F. Y. 2006. Response of wheat yield under combined use of fungicides and biofertilizer. . International Journal of Agricultural and Biology. 8:580–582.

16- Okan Y., and Labandera-Gonzalez C. A. 1994. Agronomic applications of Azospirillum: anevaluation of 20 years worldwide field inoculation. Soil Biology and Biochemistry. 26: 1591-1601.

17- Piotrowska-Seget Z., Engel R., Nowak E., and Kozdroj J. 2008. Successive soil treatment with Captan or oxytetracycline affects non-target microorganisms. World Journal of Microbiology and Biotechnology. 24: 2843-2848.

18- Saleh Rastin N. 1999. Biological fertilizers, Soil and Water science journal. 12: 1-36. (in Persian with English abstract)

19- Tilak K. V. B. R., Ranganayaki N., Pal K. K., De R., Saxena A. K., Shekhar Nautiyal C., Tripathi A. K., and Johri B. N. 2005. Diversity of plant growth and soil health supporting bacteria. Current Science. 89:136-150.

20- Wu S. C., Cao Z. H., Li Z. G., Cheung K. C., and Wong M. H. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125:155-166.