تأثیر ریزوبیوم بر تولید گلومالین توسط Rhizophagus irregularis در همزیستی با گیاه شبدر تحت سطوح نیتروژن

آب و خاک

مقاله 8، دوره 32، شماره 2 - شماره پیاپی 58، خرداد 1397، صفحه 399-413   

نوع مقاله: مقالات پژوهشی

شناسه دیجیتال (DOI): 10.22067/jsw.v32i2.70981

نویسندگان

وحیده شعبانی زنوزق^{* 1}؛ ناصر علی اصغرزاد²؛ جعفر مجیدی³؛ رقیه حاجی بلند¹؛ بهزاد برادران³؛ لیلی عاقبتی مالکی³

¹دانشگاه تبریز

²داشگاه تبریز

³دانشگاه علوم پزشکی تبریز

چکیده

گلومالین یک ترکیب گلیکوپروتئینی ویژه است که توسط قارچ‌های راسته گلومرال از رده گلومرومایکوتا تولید می‌شود و نقش کلیدی در ذخیره کربن آلی و نیتروژن خاک دارد. همچنین در تشکیل خاکدانه‌های پایدار و استقرار جوامع غنی میکروبی در خاک نقش بسزایی دارد. آزمایشی در قالب طرح فاکتوریل در پایه بلوک‌های کامل تصادفی با سه تکرار طراحی شد و گیاه شبدر (Trifolium repense L.)با قارچ Rhizophagus irregularis و باکتری Rhizobium leguminosarum bv. Trifolii تلقیح شد. چهار سطح نیتروژن (0، 2, 6 و 10 میلی‌مولار به فرم نیترات) بوسیله محلول غذایی نیومن و رومهلد ایجاد شد. گلدان‌ها با محلول غذایی آبیاری شدند. گیاهان شبدر پس از 12 هفته برداشت شد. گلومالین در بستر شن (SG) و گلومالین ریشه‌ای (RG) پس از استخراج از خاک، به روش بردفورد اندازه‌گیری شد. با افزایش سطوح نیتروژن میزان SG به طور معنی‌داری کاهش یافت (01/0 p

کلیدواژه‌ها

آربوسکولار؛ باکتری ریزوبیوم؛ بردفورد؛ قارچ میکوریز گلومالین؛ نیتروژن

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