The study of genetic diversity in varieties and lines of bread wheat (Triticum aestivum), using AFLP molecular markers

پژوهشهای زراعی ایران

Volume 7, Issue 1 - Serial Number 13, 2009   

Document Type: Research Article

Abstract

Bread wheat (Triticum aestivum) has the highest cultured area and production in Iran, but in recent years it looks that as a result of introduction of commercial and breeder varieties diversity of wheat varieties has been reduced. In this study AFLP molecular marker was used to investigate genetic variation of 34 wheat varieties and advanced lines and also to evaluate relationship existing within them. Seven pairs of primer combinations of EcoRI/Tru1I yielded 351 scorable bands out of which 129 bands were polymorphic. Average genetic similarity based on Nei coefficient was estimated as 0.96. Dendrogram obtained from UPGMA method specified four main groups for these 34 varieties and lines, subsequently confirmed by PCA analysis. The results indicated low level of genetic variation within commercial variesties cultivated in the country. Limited range of genetic variation suggests that the genotypes are probably of same origin and more attention should be paid for extending genetic variation of wheat varieties.

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